Articles | Volume 59, issue 2
https://doi.org/10.5194/aab-59-167-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/aab-59-167-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
Effect of purmorphamine on the mRNA expression of Sonic Hedgehog signaling downstream molecules in ovine embryo
Parisa Nadri
CORRESPONDING AUTHOR
Department of Animal Science, College of Agriculture, Isfahan University of
Technology, Isfahan 8415683111, Iran
Saeid Ansari-Mahyari
Department of Animal Science, College of Agriculture, Isfahan University of
Technology, Isfahan 8415683111, Iran
Azadeh Zahmatkesh
Department of Animal Science, College of Agriculture, Isfahan University of
Technology, Isfahan 8415683111, Iran
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Hamed Amirpour Najafabadi, Saeid Ansari Mahyari, Mohammad Ali Edriss, and Eva Strapakova
Arch. Anim. Breed., 59, 387–393, https://doi.org/10.5194/aab-59-387-2016, https://doi.org/10.5194/aab-59-387-2016, 2016
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This study aimed to use survival analysis and examine the factors affecting functional herd life (FHL) of cows in Isfahan using the Weibull proportional risk model in Isfahan to estimate of breeding values for functional herd life. The SC, milk production, age at first calving and milk period showed significant effects on LPL (P < 0.001) according to the likelihood ratio test. Although there were some fluctuations in genetic trends, an overall increase was observed which leads to a longer FHL
Related subject area
Subject: DNA markers and gene expressions | Animal: Sheep
Genetic polymorphism of the ovine MAP3K5 gene and its association with body size traits in Hu sheep of China
Genetic variation in the ovine KAP22-1 gene and its effect on wool traits in Egyptian sheep
Comparison of expression patterns of six canonical clock genes of follicular phase and luteal phase in Small-tailed Han sheep
Trehalose can effectively protect sheep epididymis epithelial cells from oxidative stress
The expression and mutation of BMPR1B and its association with litter size in small-tail Han sheep (Ovis aries)
Molecular mechanisms of fat deposition: IL-6 is a hub gene in fat lipolysis, comparing thin-tailed with fat-tailed sheep breeds
Genetic diversity and phylogenetic relationship of nine sheep populations based on microsatellite markers
Expression analysis of DIO2, EYA3, KISS1 and GPR54 genes in year-round estrous and seasonally estrous rams
Assessment of genetic diversity and differentiation among four indigenous Turkish sheep breeds using microsatellites
Assessment of genetic diversity in main local sheep breeds from Romania using microsatellite markers
Associations of ORMDL1 gene copy number variations with growth traits in four Chinese sheep breeds
Selection signature analysis reveals genes underlying sheep milking performance
Identification of the association between FABP4 gene polymorphisms and milk production traits in Sfakia sheep
Identification of genomic regions and candidate genes of functional importance for gastrointestinal parasite resistance traits in Djallonké sheep of Burkina Faso
The novel T755C mutation in BMP15 is associated with the litter size of Iranian Afshari, Ghezel, and Shal breeds
Nutritional modification of SCD, ACACA and LPL gene expressions in different ovine tissues
Using microsatellite markers to analyze genetic diversity in 14 sheep types in Iran
Effect of the IGF-I gene polymorphism on growth, body size, carcass and meat quality traits in Coloured Polish Merino sheep
A novel 29 bp insertion/deletion (indel) variant of the LHX3 gene and its influence on growth traits in four sheep breeds of various fecundity
The expression of the SCD1 gene and its correlation with fattening and carcass traits in sheep
Xiaobin Yang, Weimin Wang, Deyin Zhang, Xiaolong Li, Yukun Zhang, Yuan Zhao, Liming Zhao, Jianghui Wang, Dan Xu, Jiangbo Cheng, Wenxin Li, Bubo Zhou, Changchun Lin, Xiwen Zeng, Rui Zhai, Zongwu Ma, Jia Liu, Panpan Cui, and Xiaoxue Zhang
Arch. Anim. Breed., 66, 71–79, https://doi.org/10.5194/aab-66-71-2023, https://doi.org/10.5194/aab-66-71-2023, 2023
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The demand for mutton is increasing yearly. Sheep were raised to 180 d of age, and production performance was measured and recorded at 20-d intervals from 80 to 180 d. The sheep were then slaughtered at 180 d of age. Blood, tissues, and other samples were collected for follow-up experimental research. Finally, we detected a mutation site in a specific segment of the ovine MAP3K5 gene, and this site can be used as a molecular genetic marker to improve sheep body size traits.
Ahmed M. Sallam, Aymen A. Gad-Allah, and Essam M. Albetar
Arch. Anim. Breed., 65, 293–300, https://doi.org/10.5194/aab-65-293-2022, https://doi.org/10.5194/aab-65-293-2022, 2022
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This report provides valuable information about an interesting candidate gene underlying new genetic markers, which may be used for marker-assisted selection to improve quantity and quality of wool produced from Egyptian sheep. A part of the KAP22-1 gene was amplified, sequenced and genotyped in an Egyptian sheep population with important wool traits available for the animals for further association analysis.
Qi Han, Xiaoyun He, Ran Di, and Mingxing Chu
Arch. Anim. Breed., 64, 457–466, https://doi.org/10.5194/aab-64-457-2021, https://doi.org/10.5194/aab-64-457-2021, 2021
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Our results suggest that estrous cycles may be associated with clock gene expression in the Small-tailed Han sheep (STH sheep). This is the first study to systematically analyze the expression patterns of clock genes of different estrous cycles in ewes, which could form a basis for further studies to develop the relationship between clock genes and the estrous cycle.
Zhaojin Luan, Xiaomei Fan, Yongchao Zhao, Huizi Song, Wei Du, Jiaoxia Xu, Zhaochen Wang, Wenguang Zhang, and Jiaxin Zhang
Arch. Anim. Breed., 64, 335–343, https://doi.org/10.5194/aab-64-335-2021, https://doi.org/10.5194/aab-64-335-2021, 2021
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In this work, we analyzed the effects of trehalose on sheep EEC proliferation and possible mechanisms affecting its role. Our study suggested that exogenous trehalose exhibits antioxidant activity through increasing the activities of CAT, GSH-Px, and the expression level of GPX5. The cell line cultured provides a strong resource for elucidating the transcriptional networks that coordinate functions of sheep epididymis epithelium and have greatly enhanced our understanding of epididymis biology.
Yu-Liang Wen, Xiao-Fei Guo, Lin Ma, Xiao-Sheng Zhang, Jin-Long Zhang, Sheng-Guo Zhao, and Ming-Xing Chu
Arch. Anim. Breed., 64, 211–221, https://doi.org/10.5194/aab-64-211-2021, https://doi.org/10.5194/aab-64-211-2021, 2021
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The expression and polymorphism of the BMPR1B gene associated with litter size in small-tail Han (STH) sheep were determined. We found that BMPR1B was mainly expressed in reproduction-related tissues and was more highly expressed in the hypothalamus of polytocous than in monotocous ewes in the follicular and luteal phases. A new molecular marker was found and was negatively correlated with litter size in STH sheep. These results can provide a reference for selective sheep breeding.
Sana Farhadi, Jalil Shodja Ghias, Karim Hasanpur, Seyed Abolghasem Mohammadi, and Esmaeil Ebrahimie
Arch. Anim. Breed., 64, 53–68, https://doi.org/10.5194/aab-64-53-2021, https://doi.org/10.5194/aab-64-53-2021, 2021
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The mechanism of lipid metabolism is complex, and the manipulation of fat storage for lean meat production is very important in the sheep-breeding industry. The present study aimed to study the genetic profiles of fat tissues and to discover the diversity in the genetic mechanisms defining fat deposition between two morphologically different sheep breeds. Analysis of the diversity of fat deposition may aid the recognition of genes and pathways responsible for the formation of tail fat.
Qing Xia, Xiangyu Wang, Zhangyuan Pan, Rensen Zhang, Caihong Wei, Mingxing Chu, and Ran Di
Arch. Anim. Breed., 64, 7–16, https://doi.org/10.5194/aab-64-7-2021, https://doi.org/10.5194/aab-64-7-2021, 2021
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The objective was to assess the genetic diversity and phylogenetic relationship of nine sheep populations. Overall, these sheep populations in the study exhibited a rich genetic diversity. The nine sheep populations can be divided into two groups. SUF and DST were clustered in one group, and GMM–BAS–BAM, HUS–STH and DOS–DOP were divided into three clusters. This clustering result is consistent with sheep breeding history. TreeMix analysis also hinted at the possible gene flow from GMM to SUF.
Qing Xia, Ran Di, Xiao-Yun He, Cai-Hong Wei, and Ming-Xing Chu
Arch. Anim. Breed., 63, 451–460, https://doi.org/10.5194/aab-63-451-2020, https://doi.org/10.5194/aab-63-451-2020, 2020
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The expression patterns of the HPG axis-related genes in rams were analyzed using qPCR. It showed that DIO2 and KISS1 were mainly expressed in pituitary and hypothalamus in both breeds, respectively. EYA3 and GPR54 were widely expressed in both breeds, with significant differences in testis and vas deferens. We speculated that the four genes may regulate the estrous mode in different regions in rams. This is the first study to analyze the expression patterns of HPG axis-related genes in rams.
Bahar Argun Karsli, Eymen Demir, Huseyin Goktug Fidan, and Taki Karsli
Arch. Anim. Breed., 63, 165–172, https://doi.org/10.5194/aab-63-165-2020, https://doi.org/10.5194/aab-63-165-2020, 2020
Andreea Dudu, Gina-Oana Popa, Elena Ghiță, Rodica Pelmuș, Cristina Lazăr, Marieta Costache, and Sergiu E. Georgescu
Arch. Anim. Breed., 63, 53–59, https://doi.org/10.5194/aab-63-53-2020, https://doi.org/10.5194/aab-63-53-2020, 2020
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The state of local breeds is precarious worldwide because of the introduction of breeds with improved traits. Our study analyzes the genetic variation of four Romanian local sheep breeds using nuclear DNA markers. Tsurcana and Tsigai showed an intense gene flow among them and were less differentiated than Ratska and Teleorman Blackhead. The results of this study may be useful for breeding programs and conservation plans since the genetic resources of the local breeds must be preserved.
Xiaogang Wang, Xiukai Cao, Yifan Wen, Yilei Ma, Ibrahim Elsaeid Elnour, Yongzhen Huang, Xianyong Lan, Buren Chaogetu, Linyong Hu, and Hong Chen
Arch. Anim. Breed., 62, 571–578, https://doi.org/10.5194/aab-62-571-2019, https://doi.org/10.5194/aab-62-571-2019, 2019
Zehu Yuan, Wanhong Li, Fadi Li, and Xiangpeng Yue
Arch. Anim. Breed., 62, 501–508, https://doi.org/10.5194/aab-62-501-2019, https://doi.org/10.5194/aab-62-501-2019, 2019
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Sheep milk production and ingredients are influenced by genetic and environmental factors. In this study, we implemented selection signature analysis to identify candidate genes related to ovine milk traits. The results revealed six selection signature regions showing signs of being selected (P < 0.001) located in chromosomes 1, 2, 3, 6, 13 and 18. In addition, 38 QTLs related to sheep milk performance were identified in selection signature regions, which contain 334 candidate genes.
Adel H. M. Ibrahim, Nikolaos Tzanidakis, Smaragda Sotiraki, Huitong Zhou, and Jonathan G. H. Hickford
Arch. Anim. Breed., 62, 413–422, https://doi.org/10.5194/aab-62-413-2019, https://doi.org/10.5194/aab-62-413-2019, 2019
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Selective breeding for milk production traits in sheep using molecular marker technology offers a possibility of selecting accurately elite ewes and rams for milk production traits at early age. Our findings suggest that ovine FABP4 has a role in the variation of the milk production traits which may be of economic importance to sheep farmers. Variation in this gene could potentially be used as gene markers for milk production traits if such associations exist.
Isabel Álvarez, Iván Fernández, Albert Soudré, Amadou Traoré, Lucía Pérez-Pardal, Moumouni Sanou, Stephane A. R. Tapsoba, Nuria A. Menéndez-Arias, and Félix Goyache
Arch. Anim. Breed., 62, 313–323, https://doi.org/10.5194/aab-62-313-2019, https://doi.org/10.5194/aab-62-313-2019, 2019
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Increasing genetic resistance to gastrointestinal parasite infections in sheep would limit the use of drugs and the emergence of resistant parasites. However, this is a very complex trait. The identification of genomic areas associated with the trait is hindered by genotype–environment interactions. Therefore, it is necessary to obtain information from non-cosmopolitan sheep populations. Here, the West African Djallonké sheep genomic profile is analyzed to contribute to solve this gap.
Hamid-Reza Amini, Amir Ajaki, Majid Farahi, Mitra Heidari, Ahmad Pirali, Mohsen Forouzanfar, and Shahin Eghbalsaied
Arch. Anim. Breed., 61, 153–160, https://doi.org/10.5194/aab-61-153-2018, https://doi.org/10.5194/aab-61-153-2018, 2018
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Birth of twins is partly controlled by genetic factors. Here, we genotyped ewes with triplet births, twin births, and single births as well as two infertile ewes from Iranian sheep breeds for the BMP15 gene. A new mutation was detected in two sterile ewes and all ewes with triplet-birth lambing. There is a relationship between the mutation and higher litter numbers. However, this mutation is not responsible for sterility or triplet births in Iranian sheep.
Katarzyna Ropka-Molik, Jan Knapik, Marek Pieszka, Tomasz Szmatoła, and Katarzyna Piórkowska
Arch. Anim. Breed., 60, 243–250, https://doi.org/10.5194/aab-60-243-2017, https://doi.org/10.5194/aab-60-243-2017, 2017
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The aim of present study was the analysis of differences in expression profiles of ovine genes related to lipid metabolism (LPL, ACACA, SCD) depending on feeding system and tissue type (fat, liver). The genes expression measurement showed that supplementation of diet with an addition of fresh grass or red clover modified the expression of all genes in fat tissue. The nutrigenomic regulation of analyzed genes confirmed that these genes play a critical role in regulation of lipid metabolism.
Mohammad Taghi Vajed Ebrahimi, Mohammadreza Mohammadabadi, and Ali Esmailizadeh
Arch. Anim. Breed., 60, 183–189, https://doi.org/10.5194/aab-60-183-2017, https://doi.org/10.5194/aab-60-183-2017, 2017
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Sheep farming in Iran can be a great source of income for marginal and landless farmers. Animal genetic diversity is required to facilitate rapid adaptation to changing breeding objectives. Hence, the objective of this research was to measure the genetic polymorphism of five microsatellite markers in 14 sheep types in Iran. The high degree of variability demonstrated within these sheep implies that these populations are rich reservoirs of genetic diversity that must be preserved.
Ewa Grochowska, Bronisław Borys, Piotr Janiszewski, Jan Knapik, and Sławomir Mroczkowski
Arch. Anim. Breed., 60, 161–173, https://doi.org/10.5194/aab-60-161-2017, https://doi.org/10.5194/aab-60-161-2017, 2017
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The aim of this study was to investigate the polymorphism in the IGF-I gene and its associations with growth, body size, carcass and meat quality traits in Coloured Polish Merino sheep. The IGF-I genotype was found to have an effect on fore shank weight, kidney fat class and EUROP fat class, external fatness on carcass class, drip loss, and subjective assessment of meat colour. The IGF-I gene could be considered as a candidate gene of selected carcass and meat quality traits in sheep.
Haidong Zhao, Shuai He, Yanjiao Zhu, Xin Cao, Renyun Luo, Yong Cai, Hongwei Xu, and Xiuzhu Sun
Arch. Anim. Breed., 60, 79–85, https://doi.org/10.5194/aab-60-79-2017, https://doi.org/10.5194/aab-60-79-2017, 2017
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The 29 bp indel of sheep LHX3 gene was firstly verified in four Chinese indigenous sheep breeds of various fecundity. Genotypic frequency and allelic frequency distributions were significantly different between the high-fecundity breeds (Hu sheep, HS; small-tail Han sheep, STHS; and Lanzhou fat-tail sheep, LFTS) and the low-fecundity breed (Tong sheep (TS)) based on an χ2 test (P < 0.05). Moreover, four significant differences were found in body length and chest width in TS and STHS (P < 0.05).
K. Ropka-Molik, J. Knapik, M. Pieszka, and T. Szmatoła
Arch. Anim. Breed., 59, 37–43, https://doi.org/10.5194/aab-59-37-2016, https://doi.org/10.5194/aab-59-37-2016, 2016
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Stearoyl-CoA desaturase 1 is a critical enzyme that catalyzes the synthesis of monounsaturated fatty acids. The objective of the present study was to evaluate the level of mRNA of SCD1 gene in three different ovine tissues strongly associated with lipid homeostasis. The significant correlation between SCD1 transcript abundance and fattening and slaughtering traits (fatness traits) indicates the ability to improve important production traits in sheep via modification of expression of SCD1 gene.
Cited articles
Camous, S., Kopecny, V., and Flechon, J. E.: Autoradiographic detection of the earliest stage of [3H]-uridine incorporation into the cow embryo, Biol. Cell., 58, 195–200, 1986.
Canettieri, G., Di Marcotullio, L., Greco, A., Coni, S., Antonucci, L., Infante, P., Pietrosanti, L., De Smaele, E., Ferretti, E., Miele, E., Pelloni, M., De Simone, G., Pedone, E. M., Gallinari, P., Giorgi, A., Steinkuhler, C., Vitagliano, L., Pedone, C., Schinina, M. E., Screpanti, I., and Gulino, A.: Histone deacetylase and Cullin3-RENKCTD11 ubiquitin ligase interplay regulates Hedgehog signalling through Gli acetylation, Nat. Cell Biol. , 12, 132–191, 2010.
Crozet, N., Huneau, D., Desmedt, V., Theron, M. C., Szollosi, D., Torres, S., and Sevellec, C.: In vitro fertilization with normal development in the sheep, Gamete. Res., 16, 159–170, 1987.
Driancourt, M. A. and Thuel, B.: Control of oocyte growth and maturation by follicular cells and molecules present in follicular fluid, Reprod. Nutr. Dev. rev., 38, 345–362, 1998.
Elia, D., Madhala, D., Ardon, E., Reshef, R., and Halevy, O.: Sonic hedgehog promotes proliferation and differentiation of adult muscle cells: involvement of MAPK/ERK and PI3K/Akt pathway, Biochim. Biophy. Acta., 1773, 1438–1446, 2007.
Hooper, J. E. and Scott, M. P.: Communicating with Hedgehogs, Nat. Rev. Mol. Cell. Biol., 6, 306–317, 2005.
Ikeda, S., Ichihara-Tanaka, K., Azuma, T., Muramatsu, T., and Yamada, M.: Effects of midkine during in vitro maturation of bovine oocytes on subsequent developmental competence, Biol. Reprod., 63, 1067–1074, 2000.
Ingham, P. W. and McMahon, A. P.: Hedgehog signaling in animal development: paradigms and principles, Genes. Dev., 15, 3059–3087, 2001.
Lee, K., Jeong, J., Kwak, I., Yu, C. T., Lanske, B., Soegiarto, D. W., Toftgard, R., Tsai, M. J., Tsai, S., Lydon, J. P., and DeMayo, F. J.: Indian hedgehog is a major mediator of progesterone signaling in the mouse uterus, Nat. Genet., 38, 1204–1219, 2006.
Lee, S. J., Lindsey, S., Graves, B., Yoo, S., and Olson, J. M.: Sonic Hedgehog-induced histone deacetylase activation is required for cerebellar granule precursor hyperplasia in medulloblastoma, Plos One. 8, 714–728, 2013.
Livak, K. J. and Schmittgen, T. D.: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) method, Methods, 25, 402–408, 2001.
Matsumoto, H., Zhao, X., Das, S. K., Hogan, B. L., and Dey, S. K.: Indian hedgehog as a progesterone-responsive factor mediating epithelial- mesenchymal interactions in the mouse uterus, Dev. Biol., 245, 280–290, 2002.
McGraw, S., Robert, C., Massicotte, L., and Sirard, M. A.: Quantification of histone acetyltransferase and histone deacetylase transcripts during early bovine embryo development, Biol. Reprod., 68, 383–389, 2003.
Mehnert, J. M. and Kelly W. K.: Histone deacetylase inhibitors: biology and mechanism of action, Cancer J., 13, 23–29, 2007.
Nguyen, N. T., Lin, D. P. C., Yen, S. Y., Tseng, J. K., Chuang, J. F., Chen, B. Y., Lin, T. A., Chang, H. H., and Ju, J. C.: Sonic hedgehog promotes porcine oocyte maturation and early embryo development, Reprod. Fertil. Dev., 21, 805–815, 2009.
Nguyen, N. T., Lin, D. P. C., Siriboon, C., Lo, N. W., and Ju, J. C.: Sonic hedgehog improves in vitro development of porcine parthenotes and handmade cloned embryos, Sci. Direct. Therio., 74, 1149–1160, 2010.
Nguyen, N. T., Lo, N. W., Chuang, S. P., Jian, Y. L., and Ju, J. C.: Sonic hedgehog supplementation of oocyte and embryo culture media enhances development of IVF porcine embryos, Reproduction, 142, 87–97, 2011.
Pangas, S. A.: Growth factors in ovarian development, Semin. Reprod . Med., 25, 225–34, 2007.
Ren, Y., Cowan, R. G., Harman, R. M., and Quirk, S. M.: Dominant activation of hedgehog signaling pathway in the ovary alters theca development and prevents ovulation, Mol. Endocrinol., 23, 711–723, 2009.
Ruiz i Altaba, A., Sánchez, P., and Dahmane, N.: Gli and hedgehog in cancer: tumours, embryos and stem cells, Nat. Rev. Cancer., 2, 361–372, 2002.
Russell, M. C., Cowan, R. G., Harman R. M., Walker, A. L., and Quirk, S. M.: The hedgehog signaling pathway in the mouse ovary, Biol. Reprod., 77, 226–236, 2007.
Spicer, L. J., Sudo, S., Aad, P. Y., Wang, L. S., Chun, S. Y., Ben-Shlomo, I., Klein, C., and Hsueh, A. J. W.: The hedgehog-patched signaling pathway and function In the mammalian ovary: a novel role for hedgehog proteins in Stimulating proliferation and steroidogenesis of theca cells, Reproduction, 138, 329–339, 2009.
Stebbins-Boaz, B., Hake, L. E., and Richter, J. D.: CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus, Embo. J., 15, 2582–2592, 1996.
Takamato, N., Zhao, B. H., Tsai, S. Y., and Demayo, F. J.: Identification of Indian hedgehog as a progesterone-responsive gene in the murine uterus, Mol. Endocrinol., 16, 2338–2348, 2002.
Tervit, H. R., Whittingham, D. G., and Rowson, L. E. A.: Successful culture of sheep and cattle ova, J. Reprod. Fertil., 30, 493–497, 1972.
Thompson, J. G., Gardner, D. K., Pugh, P. A., McMillan, W. H., and Tervit, H. R.: Lamb birth weight is affected by culture system utilized during in vitro pre-elongation development of ovine embryos, Biol. Reprod., 53, 1385–1391, 1995.
Wijgerde, M., Ooms, M., Hoogerbrugge, J. W., and Grootegoed, J. A.: Hedgehog signaling in mouse ovary: Indian hedgehog and desert hedgehog from granulosa cells induce target gene expression in developing theca cells, Endocrinology, 146, 3558–3566, 2005.
Wu, X., Ding, S., Ding, Q., Gray, N. S., and Schultz, P. G.: A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells, J. Am. Chem. Soc., 124, 14520–14521, 2002.
Zuccotti, M., Boiani, M., Ponce, R., Guizzardi, S., Scandroglio, R., and Garagna, S.: Mouse Xist expression begins at zygotic genome activation and is timed by a zygotic clock, Mol. Reprod. Dev., 61, 14–20, 2002.
Short summary
In this research, the effect of purmorphamine on mRNA expression of the SHH signaling downstream molecules Ptch1, Gli1, Smo, Hdac1, Hdac2 and Hdac3 in ovine two-cell embryos was studied. Regarding the presence of SHH signaling molecules in two-cell embryos and their response to purmorphamine, it can be suggested that SHH signaling is probably active before embryonic genome activation in ovine embryos.
In this research, the effect of purmorphamine on mRNA expression of the SHH signaling downstream...