This research aimed to investigate the differentially expressed miRNAs related to uterine involution in ovary and uterus. We have screened a total of 16 differentially expressed miRNAs in ovary libraries and 54 miRNAs in uterus libraries. Meanwhile, we have found that miRNA-200a could target ZEB1 and YAP1 to participate in the process of uterine involution. The study enriched the miRNA sets related to the uterine involution in the ovary–uterus axis.
In this work, alpaca wool thickness and medullation characteristics were analyzed based on the animals' sex and color. The wool of females was thinner and had a lower medullation percentage than for males. The share of non-medullated fibers in the area up to 30 µm was also greater in the wool of females than for males. Light wool was thinner and had a lower degree of medullation than dark wool. The discontinuous and continuous medullated light fibers were thinner than those of dark fibers.
In this experiment, by detecting the mutation in the promoter region of the bovine TRDMT1 gene, fluorescent recombinant plasmids of different genotypes were constructed at this site, and the relative fluorescence intensity was analyzed by detecting the transfected 293T cells. Our experiment detected a natural genetic variation of a tRNA modification gene TRDMT1, which may provide potential natural molecular materials for the study of tRNA modification.
The redox potential of goat kid serum was evaluated, applying spectrophotometric assays intended for both clinical and research studies that can be automated on clinical auto-analysers, thus allowing rapid and inexpensive data collections. Although several correlations were shown, the results obtained with different assays can be strongly different depending on the method and technology of each assay. The present results can be useful to verify whether animals are experiencing oxidant status.
This research aimed to investigate the molecular regulatory mechanisms involving miRNAs during uterine involution in postpartum ewes. We have screened a total of 118 differentially expressed miRNAs in hypothalamic libraries and 54 miRNAs in uterine libraries. Meanwhile, we have found two miRNA-target gene pairs that might participate in uterine involution in postpartum ewes. The study fills a gap in the research related to miRNAs in uterine involution in female livestock, especially in sheep.
Water-holding capacity (WHC) is a meat quality trait that affects economic outcomes caused by the tissue fluid loss and weight loss. The present study indicates that the mutation c.-379T>G in the UBXN1 promoter is associated with promoter activity, UBXN1 mRNA level, and WHC. Therefore, it potentially contributes to WHC improvement by regulating the degradation of myofibrillar proteins. It is a promising marker for selection of pork quality and increases economic benefits of the pork industry.