Articles | Volume 68, issue 2
https://doi.org/10.5194/aab-68-271-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/aab-68-271-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Original study
| 
08 May 2025
Original study |
| 08 May 2025
| 08 May 2025
FASN thioesterase domain mutation and fatty acid profile in Holstein Friesian intramuscular fat
David Cancino-Baier
CORRESPONDING AUTHOR
Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, 01145 Francisco Salazar Ave, Temuco, Chile
Centro de Excelencia en Física e Ingeniería en Salud, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, 01145 Francisco Salazar Ave, Temuco, Chile
Erwin Muñoz-Acuña
Centro de Excelencia en Biotecnología de la Reproducción (CEBIOR-BIOREN), Universidad de La Frontera, 01145 Francisco Salazar Ave, Temuco, Chile
Departamento de Producción Agropecuaria. Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, 01145 Francisco Salazar Ave, Temuco, Chile
John Quiñones
Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, 01145 Francisco Salazar Ave, Temuco, Chile
Rommy Diaz
Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, 01145 Francisco Salazar Ave, Temuco, Chile
Jorge F. Beltrán
Programa de Doctorado en Ciencias Mención Biología Celular y Molecular Aplicada, Universidad de La Frontera, 01145 Francisco Salazar Ave, Temuco, Chile
Jorge Farías
Departamento de Ingeniería Química, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, 01145 Francisco Salazar Ave, Temuco, Chile
Karla Inostroza
Departamento de Ciencias Agropecuarias y Acuícolas, Facultad de Recursos Naturales, Universidad Católica de Temuco, Temuco 4780000, Chile
Rodrigo Huaiquipan
Programa de Doctorado en Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, 01145 Francisco Salazar Ave, Temuco, Chile
José Bento Sterman Ferraz
Grupo de Melhoramento Animal, Departamento de Ciencias Baìsicas, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil
Néstor Sepúlveda
Departamento de Producción Agropecuaria. Facultad de Ciencias Agropecuarias y Medioambiente, Universidad de La Frontera, 01145 Francisco Salazar Ave, Temuco, Chile
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Coat-colour-related genotypes, phenotyping and biometric assessment of three ecotypes of pigs in Cameroon
Identification of a novel InDel locus within the ACSL5 gene and its association with body measurement traits in goats
Correlation and regression analysis of the KRT27 and ELOVL4 genes in cashmere fineness and other production performances in Liaoning cashmere goats
Genome-wide association study of the antibody response to Corynebacterium pseudotuberculosis in sheep
The study of miRNA-211 affects melanogenesis progress in Cashmere goats via suppressing AP1S2
Exploring the association between SLC11A1 and CARD15 gene polymorphisms and tuberculosis susceptibility in Holstein cattle
Genetic analysis of platelet-derived growth factor receptor-like gene (PDGFRL) polymorphism and melanin traits in Lanping black-boned sheep (Ovis aries)
Investigating InDels in YAP and TAZ genes and their impact on growth characteristics in goats
The genetic diversity and structure of the Haflinger horse population in the Czech Republic
Evaluation of genetic diversity within different rabbit (Oryctolagus cuniculus) genotypes utilizing start codon targeted (SCoT) and inter-simple sequence repeat (ISSR) molecular markers
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Arch. Anim. Breed., 68, 239–251, https://doi.org/10.5194/aab-68-239-2025, https://doi.org/10.5194/aab-68-239-2025, 2025
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Cameroon local pigs are out-bred and neglected ecotypes, with a consistent variation in coat colours and colour patterns. The phenotypic diversity is an asset for selective breeding and genetic improvement. But it is challenging for accurate information sharing for breed utilisation, improvement and conservation perspectives. This study aimed to describe the genetic resources of pigs and to develop a standard protocol for phenotypic information sharing with the global scientific audience.
Ao Wang, Xianfeng Wu, Qian Xu, Benzhi Wang, Tianfang Xiao, Yuan Liu, and Wenyang Li
Arch. Anim. Breed., 68, 171–181, https://doi.org/10.5194/aab-68-171-2025, https://doi.org/10.5194/aab-68-171-2025, 2025
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This study investigated the spatiotemporal expression pattern of ACSL5 (acyl-CoA synthetase 5) in goats and the association between its polymorphism and body measurement traits. We found that the ACSL5 gene was expressed ubiquitously in the nine tested tissues of Fuqing goats. The del41712 locus of the ACSL5 gene has a significant impact on multiple body measurement traits of goats, providing a basis for the application of marker-assisted selection in goat breeding.
Hua Ma, Weihang Hong, Lingjun Nie, Shuaitong Li, Qingyu Yuan, Ran Duan, Qiying Zhan, Lingchao Kong, and Zeying Wang
Arch. Anim. Breed., 68, 135–149, https://doi.org/10.5194/aab-68-135-2025, https://doi.org/10.5194/aab-68-135-2025, 2025
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This study investigated single-nucleotide polymorphism (SNP) in the keratin 27 (KRT27, 1919G/A) and elongation of very-long-chain fatty acids 4 (ELOVL4, 28666C/T) genes, which are associated with production traits in Liaoning cashmere goats. Multivariate analysis was also conducted. The GGCC genotype combination was associated with finer cashmere quality. The GGTT haplotype exhibited a multigenic effect. The TT genotype demonstrated pleiotropy.
Jitka Kyselová, Ladislav Tichý, Jiřina Marková, Artur Gurgul, Zuzana Sztankóová, Karel Vališ, Soňa Šlosárková, Kateřina Kavanová, Monika Beinhauerová, and Tomasz Szmatola
Arch. Anim. Breed., 68, 109–124, https://doi.org/10.5194/aab-68-109-2025, https://doi.org/10.5194/aab-68-109-2025, 2025
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This study explored how genetics might influence the ability of sheep to fight a severe infection known as caseous lymphadenitis (CLA). By examining the genome of 321 Suffolk sheep, we identified specific genetic markers indicating biological processes and pathways that might be associated with the resistance of some sheep to this disease. This discovery is a step towards breeding sheep that are naturally more capable of fighting off CLA, potentially leading to healthier flocks.
Baoyu Zhang, Runlai Liu, Tao Ma, Xinyu Li, Yuxin Zhao, Huixian Su, Jianping Li, Yumei Li, Huaizhi Jiang, and Qiaoling Zhang
Arch. Anim. Breed., 68, 125–134, https://doi.org/10.5194/aab-68-125-2025, https://doi.org/10.5194/aab-68-125-2025, 2025
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Coat color is an important economic trait of Cashmere goats. miRNA-211 is differentially expressed in the skin of Cashmere goats with different coat colors, but the mechanism is unclear. After the targeting relationship between miRNA-211 and AP1S2 was clarified in vitro and in vivo, antagomiRNA-211 was injected into BALB/c, and the expression of miRNA-211 and AP1S2 and melanin content was analyzed. It was demonstrated that miRNA-211 impacts melanogenesis by downregulating AP1S2.
Safa Bejaoui, Nour Elhouda Fehri, Mohamed Amine Ferchichi, and Bayrem Jemmali
Arch. Anim. Breed., 67, 515–521, https://doi.org/10.5194/aab-67-515-2024, https://doi.org/10.5194/aab-67-515-2024, 2024
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Genetic variation in the SLC11A1 (C>G) and CARD15 (A/G) genes may contribute to the onset and development of bovine tuberculosis (bTB), supporting the hypothesis that polymorphisms in these genes are associated with the risk of bTB in Holstein cattle. bTB is a complicated disease that is probably influenced by polymorphisms in many genes, particularly those related to the immune system and host–pathogen interactions.
Dan Yue, Chaochao Peng, Sameeullah Memon, Azeem Iqbal, Heli Xiong, Xiaoming He, Ying Lu, and Weidong Deng
Arch. Anim. Breed., 67, 383–392, https://doi.org/10.5194/aab-67-383-2024, https://doi.org/10.5194/aab-67-383-2024, 2024
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We focus on the Lanping black-boned sheep (LPBB) because it is the only mammal that resembles the Chinese silky fowl in the world. LPBB is characterized by black pigmentation in its plasma colorimetry and had its internal organs compared to the reddish color in Lanping normal sheep. Although the literature is abundant in genetic case studies, the processes of pigmentation remain obscure. We thus investigate the association between the PDGFRL gene polymorphism of LPBB and melanin deposition.
Wanxia Zhao, Ziteng Wang, Yichen Lei, Xiaoqin Tang, Xiaohua Yi, Junyi Jiang, Jiapeng Li, Shuhui Wang, and Xiuzhu Sun
Arch. Anim. Breed., 67, 343–351, https://doi.org/10.5194/aab-67-343-2024, https://doi.org/10.5194/aab-67-343-2024, 2024
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In this study, InDels loci of YAP and TAZ genes in two goat breeds were screened and identified, and the association between InDels loci of YAP and TAZ genes and growth traits was analyzed. The results showed that eight InDels loci were identified from goat YAP and TAZ genes. These InDels loci significantly affected growth traits such as chest width, body height, and body weight of goats. These findings can provide new molecular markers for goat molecular breeding.
Michaela Kulišťáková, Iva Jiskrová, Irena Vrtková, Petra Bartoňová, and Tomáš Urban
Arch. Anim. Breed., 67, 323–333, https://doi.org/10.5194/aab-67-323-2024, https://doi.org/10.5194/aab-67-323-2024, 2024
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The aim of this study is to assess the genetic diversity and structure of the Haflinger horse population in the Czech Republic based on 16 microsatellite loci. The results indicated a high level of diversity and the presence of greater admixture, and the identification of distinct genetic clusters suggested a significant gene flow. These findings imply that the population exhibits sufficient genetic variability and diversity.
Nouran Elsayed, Abd Elrahman E. Mandour, Mamdouh K. A. Amin, Fayiz M. Reda, Heba S. A. Taha, Alessandro Di Cerbo, Mahmoud M. Azzam, and Mahmoud Alagawany
Arch. Anim. Breed., 67, 285–295, https://doi.org/10.5194/aab-67-285-2024, https://doi.org/10.5194/aab-67-285-2024, 2024
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It was suggested that the start codon targeted (SCoT) markers may be more effective than inter-simple sequence repeat (ISSR) for differentiating and identifying the genetic variations within investigated breeds. Also, the usage of molecular markers of SCoT and ISSR may be more proper for calculating genetic diversity and common ancestry among tested rabbit breeds.
Rebecca Simon, Karólína Elísabetardóttir, and Gesine Lühken
Arch. Anim. Breed., 67, 237–246, https://doi.org/10.5194/aab-67-237-2024, https://doi.org/10.5194/aab-67-237-2024, 2024
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Genetics of horn traits in sheep are still under investigation. Icelandic sheep were chosen to further characterize this complex trait using known variants as it is highly variable for horn characteristics. Only the published variant for multi-hornedness was in perfect segregation. The absence of horns was analyzed in multi-horned sheep. Only tendencies of association were found for hornlessness and for different horn shapes and sizes, confirming previous results in other breeds.
Lige Huang, Dan Sheng, Xinyang Fan, Ruixia Gao, and Yongwang Miao
Arch. Anim. Breed., 67, 217–230, https://doi.org/10.5194/aab-67-217-2024, https://doi.org/10.5194/aab-67-217-2024, 2024
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The aim of this study was to investigate the role of TP53 in buffalo lactation. The data showed that buffalo TP53 inhibited the expression of genes related to milk protein and milk fat in buffalo mammary epithelial cells by inhibiting the PI3K–AKT–mTOR pathway. These results suggest that TP53 negatively regulates the synthesis of milk protein and milk fat in buffalo through the PI3K–AKT–mTOR pathway. This study provides new insights into the functional role of TP53 in buffalo lactation.
Serdar Koçak, Mustafa Tekerli, Metin Erdoğan, Koray Çelikeloğlu, Ebubekir Yazıcı, Zehra Bozkurt, Özlem Hacan, Mustafa Demirtaş, and Samet Çinkaya
Arch. Anim. Breed., 67, 207–216, https://doi.org/10.5194/aab-67-207-2024, https://doi.org/10.5194/aab-67-207-2024, 2024
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It was detected that Texel and myostatin-carrying BC1 lambs were significantly higher than Pırlak and non-carrying BC1 lambs in the MLD area at weaning. Myostatin-carrying BC1 lambs had a superiority to Pırlak lambs in terms of muscle conformation and fatness of the hind leg. Myostatin-carrying BC1 lambs were found to be better than Pırlak in growth performance. It was concluded that the Pırlak lambs may be improved by introgression of myostatin mutation.
Jinxin Wang, Yufang Liu, Siwu Guo, Ran Di, Xiangyu Wang, Xiaoyun He, and Mingxing Chu
Arch. Anim. Breed., 67, 51–60, https://doi.org/10.5194/aab-67-51-2024, https://doi.org/10.5194/aab-67-51-2024, 2024
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Finding polymorphisms of genes can provide potential molecular genetic markers for sheep reproduction. This study investigated the polymorphisms of three major reproductive genes (BMPR1B, BMP15 and GDF9) in Chinese sheep and found that BMPR1B and GDF9 might be the major genes influencing the reproductive performance of Chinese sheep breeds. This result might contribute to improving the prolificacy traits of native Chinese sheep in the future.
Wei Wang, Yongjuan Wang, Yufang Liu, Guiling Cao, Ran Di, Jinyu Wang, and Mingxing Chu
Arch. Anim. Breed., 66, 411–419, https://doi.org/10.5194/aab-66-411-2023, https://doi.org/10.5194/aab-66-411-2023, 2023
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In this study, GLUD1 single nucleotide polymorphisms (SNPs) were analyzed in early-puberty goats and delayed-puberty goat breeds by sequencing and restriction fragment length polymorphism (RFLP). The present study aimed to find genetic markers related to sexual precocity and high fertility in goats and to provide a scientific basis for marker-assisted early selection of reproductive performance.
Zühal Gündüz and Osman Biçer
Arch. Anim. Breed., 66, 369–378, https://doi.org/10.5194/aab-66-369-2023, https://doi.org/10.5194/aab-66-369-2023, 2023
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The Kilis goat has emerged as a breed with a unique genetic profile that reflects its complex ancestry due to historical crossbreeding between the Damascus and Hair goat populations in Türkiye. The Kilis goat is well adapted to arid areas and is known for its high milk yield. Therefore, it holds great potential for milk production and genetic improvement programs in Türkiye. For this reason, some genes that affect milk yield were investigated.
Kunlong Qi, Yaqing Dou, Chenlei Li, Yingke Liu, Chenglei Song, Xinjian Li, Kejun Wang, Ruimin Qiao, Xiuling Li, Feng Yang, and Xuelei Han
Arch. Anim. Breed., 66, 285–298, https://doi.org/10.5194/aab-66-285-2023, https://doi.org/10.5194/aab-66-285-2023, 2023
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With the rapid development of sequencing technology, it has been found that circRNAs play an important role in skeletal muscle development. During the growth and development of livestock, the growth and development of muscle fibers are closely related to the number, proliferation, and differentiation capacity of skeletal muscle satellite cells, thereby affecting meat quality. Notably, more and more studies now suggest that changes in circRNA expression may be associated with meat quality.
Dwi Lestari, Sri Murtini, Niken Ulupi, Asep Gunawan, and Cece Sumantri
Arch. Anim. Breed., 66, 275–283, https://doi.org/10.5194/aab-66-275-2023, https://doi.org/10.5194/aab-66-275-2023, 2023
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IPB-D2 chickens are one of the local Indonesian chickens that are being developed today. IPB-D2 chickens have disease-resistant traits. Selection is not only carried out conventionally but is also developed by molecular selection methods. By utilizing gene diversity in the major histocompatibility complex gene group, it was found that there were several single-nucleotide polymorphisms in the B Locus Beta 2 gene significantly associated with immunoglobulin Y concentrations in IPB-D2 chickens.
Cited articles
Abe, T., Saburi, J., Hasebe, H., Nakagawa, T., Misumi, S., Nade, T., Nakajima, H., Shoji, N., Kobayashi, M., and Kobayashi, E.: Novel mutations of the FASN gene and their effect on fatty acid composition in japanese black beef, Biochem. Genet., 47, 397–411, https://doi.org/10.1007/s10528-009-9235-5, 2009.
Alim, M. A., Wang, P., Wu, X. P., Li, C., Cui, X. G., Zhang, S. L., Zhang, Q., Zhang, Y., and Sun, D. X.: Effect of FASN gene on milk yield and milk composition in the Chinese Holstein dairy population, Anim. Genet., 45, 111–113, https://doi.org/10.1111/age.12089, 2014.
Arnaudova, M., Brunner, T. A., and Götze, F.: Examination of students' willingness to change behaviour regarding meat consumption, Meat Sci., 184, 108695, https://doi.org/10.1016/j.meatsci.2021.108695, 2022.
Bhuiyan, M. S. A., Yu, S. L., Jeon, J. T., Yoon, D., Cho, Y. M., Park, E. W., Kim, N. K., Kim, K. S., and Lee, J. H.: DNA polymorphisms in SREBF1 and FASN genes affect fatty acid composition in Korean cattle (Hanwoo), Asian-Australas. J. Anim. Sci., 22, 765–773, https://doi.org/10.5713/ajas.2009.80573, 2009.
Cancino-Baier, D., Muñoz, E., Quiñones, J., Beltrán, J. F., Fuentes, F., Farías, J., Lorenzo, J. M., Diaz, R., Inostroza, K., and Ferraz, J. B. S.: A Non-Synonymous Single Nucleotide Polymorphism in Gene Alters FASN Enzyme Activity in Subcutaneous and Intramuscular Adipose Tissue in Holstein Friesian Steers, Ann. Anim. Sci., 21, 109–124, 2021.
Chakravarty, B., Gu, Z., Chirala, S. S., Wakil, S. J., and Quiocho, F. A.: Human fatty acid synthase: Structure and substrate selectivity of the thioesterase domain, P. Natl. Acad. Sci. USA, 101, 15567–15572, https://doi.org/10.1073/pnas.0406901101, 2004.
Ciecierska, D., Frost, A., Grzesiak, W., Proskura, W. S., Dybus, A., and Olszewski, A.: The influence of fatty acid synthase polymorphism on milk production traits in Polish Holstein-Friesian cattle, J. Anim. Plant Sci., 23, 376–379, 2013.
Dagevos, H. and Verbeke, W.: Meat consumption and flexitarianism in the Low Countries, Meat Sci., 192, 108894, https://doi.org/10.1016/j.meatsci.2022.108894, 2022.
Dallakyan, S. and Olson, A. J.: Small-molecule library screening by docking with PyRx, Methods in Molecular Biology, 243–250, https://doi.org/10.1007/978-1-4939-2269-7_19, 2015.
De Smet, S. and Vossen, E.: Meat: The balance between nutrition and health. A review, Meat Sci., 120, 145–156, https://doi.org/10.1016/j.meatsci.2016.04.008, 2016.
Folch, J., Lees, M., and Stanley, G. H. S.: A simple method for the isolation and purification of total lipids from animal tissues, J. Biol. Chem., 226, 497–509, https://doi.org/10.1007/s10858-011-9570-9, 1957.
Font-i-Furnols, M. and Guerrero, L.: Spanish perspective on meat consumption and consumer attitudes, Meat Sci., 191, 108874, https://doi.org/10.1016/j.meatsci.2022.108874, 2022.
Graça, J., Godinho, C. A., and Truninger, M.: Reducing meat consumption and following plant-based diets: Current evidence and future directions to inform integrated transitions, Trends Food Sci. Technol., 91, 380–390, https://doi.org/10.1016/j.tifs.2019.07.046, 2019.
Heo, L., Park, H., and Seok, C.: GalaxyRefine: Protein structure refinement driven by side-chain repacking, Nucleic Acids Res., 41, W384–W388, https://doi.org/10.1093/nar/gkt458, 2013.
Hu, M., Wu, P., Guo, A., and Liu, L.: Myristic acid regulates triglyceride production in bovine mammary epithelial cells through the ubiquitination pathway, Agriculture, 13, 1870, https://doi.org/10.3390/agriculture13101870, 2023.
Inostroza, K., Larama, G., and Sepúlveda, N.: Fatty Acid Composition (MUFA and CLA) in Bovine Muscle Tissue Related with the Presence of the g. 878TC SCD Gene Polymorphism, Int. J. Morphol., 30, 934–936, https://doi.org/10.4067/S0717-95022012000300028, 2012.
Inostroza, K., Larama, G., and Sepúlveda, N.: Polimorfismo G.17924a>G en el gen FASN y su relación con la composición de ácidos grasos (MUFA Y CLA) en la carne de novillos Aberdeen Angus, Revista Científica, 23, 329–333, 2013.
Joshi, A. K., Rangan, V. S., Witkowski, A., and Smith, S.: Engineering of an active animal fatty acid synthase dimer with only one competent subunit, Chem. Biol., 10, 169–173, https://doi.org/10.1016/S1074-5521(03)00023-1, 2003.
Kelley, L. A., Mezulis, S., Yates, C. M., Wass, M. N., and Sternberg, M. J. E.: The Phyre2 web portal for protein modeling, prediction and analysis, Nat. Protoc., 10, 845–858, https://doi.org/10.1038/nprot.2015.053, 2015.
Knaapila, A., Michel, F., Jouppila, K., Sontag-Strohm, T., and Piironen, V.: Millennials' Consumption of and Attitudes toward Meat and Plant-Based Meat Alternatives by Consumer Segment in Finland, Foods, 11, 456, https://doi.org/10.3390/foods11030456, 2022.
Leroy, F., Abraini, F., Beal, T., Dominguez-Salas, P., Gregorini, P., Manzano, P., Rowntree, J., and van Vliet, S.: Animal board invited review: Animal source foods in healthy, sustainable, and ethical diets – An argument against drastic limitation of livestock in the food system, animal, 16, 100457, https://doi.org/10.1016/j.animal.2022.100457, 2022.
López-Pedrouso, M., Lorenzo, J. M., Gullón, B., Campagnol, P. C. B., and Franco, D.: Novel strategy for developing healthy meat products replacing saturated fat with oleogels, Curr. Opin. Food Sci., 40, 40–45, https://doi.org/10.1016/j.cofs.2020.06.003, 2021.
Maharani, D., Jung, Y., Jung, W. Y., Jo, C., Ryoo, S. H., Lee, S. H., Yeon, S. H., and Lee, J. H.: Association of five candidate genes with fatty acid composition in Korean cattle, Mol. Biol. Rep., 39, 6113–6121, https://doi.org/10.1007/s11033-011-1426-6, 2012.
Mattick, J. S., Nickless, J., Mizugaki, M., Yang, C. Y., Uchiyama, S., and Wakil, S. J.: The architecture of the animal fatty acid synthetase. II. Separation of the core and thioesterase functions and determination of the N-C orientation of the subunit, J. Biol. Chem., 258, 15300–15304, https://doi.org/10.1016/s0021-9258(17)43806-3, 1983.
Nafikov, R., Schoonmaker, J., Reecy, J. M., Spurlock, D. M., Beitz, D. C., Koehler, K. J., and Minick-Bormann, J.: Effects of A17924G Genotypes Associated with Thioesterase Domain of Fatty Acid Synthase and K232A Genotypes of Diacylglycerol Acyltransferase-1 on Milk Fatty Acid Composition in Holstein Dairy Cows, Animal Industry Report, 655, 56, https://doi.org/10.31274/ans_air-180814-983, 2009.
Oh, D., Lee, Y., La, B., Yeo, J., Chung, E., Kim, Y., and Lee, C.: Fatty acid composition of beef is associated with exonic nucleotide variants of the gene encoding FASN, Mol. Biol. Rep., 39, 4083–4090, https://doi.org/10.1007/s11033-011-1190-7, 2012.
Pazirandeh, M., Chirala, S. S., Huang, W. Y., and Wakil, S. J.: Characterization of recombinant thioesterase and acyl carrier protein domains of chicken fatty acid synthase expressed in Escherichia coli, J. Biol. Chem., 264, 18195–18201, https://doi.org/10.1016/S0021-9258(19)84696-3, 1989.
Pećina, M., Konjačić, M., Ugarković, N. K., and Ivanković, A.: Effect of FASN, SCD, and GH Genes on Carcass Fatness and Fatty Acid Composition of Intramuscular Lipids in F1 Holstein × Beef Breeds, Agriculture, 13, 571, https://doi.org/10.3390/agriculture13030571, 2023.
Pence, H. E. and Williams, A.: ChemSpider: An Online Chemical Information Resource, J. Chem. Educ., 87, 1123–1124, https://doi.org/10.1021/ed100697w, 2010.
Realini, C. E., Ares, G., Antúnez, L., Brito, G., Luzardo, S., del Campo, M., Saunders, C., Farouk, M. M., and Montossi, F. M.: Meat insights: Uruguayan consumers' mental associations and motives underlying consumption changes, Meat Sci., 192, 108901, https://doi.org/10.1016/j.meatsci.2022.108901, 2022.
Teixeira, A. and Rodrigues, S.: Consumer perceptions towards healthier meat products, Curr. Opin. Food Sci., 38, 147–154, https://doi.org/10.1016/j.cofs.2020.12.004, 2021.
The UniProt: UniProt: a worldwide hub of protein knowledge, Nucleic Acids Res., 47, 506–515, https://doi.org/10.1093/nar/gky1049, 2018.
Trott, O. and Olson, A. J.: AutoDock Vina: Improving the Speed and Accuracy of Docking with a New Scoring Function, Efficient Optimization, and Multithreading, J. Comput. Chem., 31, 455–461, https://doi.org/10.1002/jcc.21334, 2009.
Wang, M., Ma, H., Song, Q., Zhou, T., Hu, Y., Heianza, Y., Manson, J. A. E., and Qi, L.: Red meat consumption and all-cause and cardiovascular mortality: results from the UK Biobank study, Eur. J. Nutr., 61, 2543–2553, https://doi.org/10.1007/s00394-022-02807-0, 2022.
Waterhouse, A., Bertoni, M., Bienert, S., Studer, G., Tauriello, G., Gumienny, R., Heer, F. T., De Beer, T. A. P., Rempfer, C., Bordoli, L., Lepore, R., and Schwede, T.: SWISS-MODEL: Homology modelling of protein structures and complexes, Nucleic Acids Res., 46, 296–303, https://doi.org/10.1093/nar/gky427, 2018.
Whitton, C., Bogueva, D., Marinova, D., and Phillips, C. J. C.: Are we approaching peak meat consumption? Analysis of meat consumption from 2000 to 2019 in 35 countries and its rela-tionship to gross domestic product, Animals, 11, 3466, https://doi.org/10.3390/ani11123466, 2021.
Xie, Y., Ma, Y., Cai, L., Jiang, S., and Li, C.: Reconsidering Meat Intake and Human Health: A Review of Current Research, Mol. Nutr. Food Res., 66, 2101066, https://doi.org/10.1002/mnfr.202101066, 2022.
Zhang, S., Knight, T. J., Reecy, J. M., and Beitz, D. C.: DNA polymorphisms in bovine fatty acid synthase are associated with beef fatty acid composition, Anim. Genet., 39, 62–70, https://doi.org/10.1111/j.1365-2052.2007.01681.x, 2008.
Zhang, S., Liu, T., Brown, M. A., and Wu, J. P.: Comparison of longissimus dorsi fatty acids profiles in Gansu Black Yak and Chinese Yellow Cattle Steers and Heifers, Korean J. Food Sci. An., 35, 286, https://doi.org/10.5851/kosfa.2015.35.3.286, 2015.
Short summary
A mutation in the thioesterase domains of the fatty acid synthase enzyme reduces the percentage of myristic acid. In silico simulations were performed to assess the mutation's impact on the catalytic site's affinity for ligands and its effect on intramuscular fatty acid profiles. Tertiary structures were generated, revealing increased affinity between the mutated enzyme and myristoyl. However, the structural changes have a more significant impact on the fatty acid profile than the altered affinity.
A mutation in the thioesterase domains of the fatty acid synthase enzyme reduces the percentage...
