Articles | Volume 67, issue 2
https://doi.org/10.5194/aab-67-217-2024
© Author(s) 2024. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/aab-67-217-2024
© Author(s) 2024. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Molecular characterization, function, tissue differential expression, and single-nucleotide polymorphism of buffalo TP53 gene
Lige Huang
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China
Dan Sheng
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China
Xinyang Fan
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China
Ruixia Gao
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China
Yongwang Miao
CORRESPONDING AUTHOR
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China
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Xinyang Fan, Shanshan Gao, Lin Fu, Lihua Qiu, and Yongwang Miao
Arch. Anim. Breed., 63, 345–354, https://doi.org/10.5194/aab-63-345-2020, https://doi.org/10.5194/aab-63-345-2020, 2020
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The αS2 casein (αS2-CN) is an important component in milk, but the variation of buffalo CSN1S2 and its corresponding variants are not clear. In this study, 13 single nucleotide polymorphisms were identified in CSN1S2 by DNA sequencing. Based on them, 11 αS2-CN variants were inferred and named. There are eight amino acid differences between buffalo and cattle in the sequences of αS2-CN variants. The results provide insights into the variation, characteristics and function of buffalo CSN1S2.
Lihua Qiu, Xinyang Fan, Yongyun Zhang, Xiaohong Teng, and Yongwang Miao
Arch. Anim. Breed., 63, 249–259, https://doi.org/10.5194/aab-63-249-2020, https://doi.org/10.5194/aab-63-249-2020, 2020
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In order to reveal whether peroxisome proliferator-activated receptor γ coactivator-1 α (PPARGC1A) gene is involved in the process of milk fat synthesis in buffalo, we analyzed the function and expression of this gene here. The results showed that buffalo PPARGC1A played a key role in the lipid synthesis of mammary gland. Eight SNPs were found in its coding region, of which three were non-synonymous. This study revealed that the PPARGC1A is closely related to buffalo milk fat synthesis.
Fangting Zhou, Yongyun Zhang, Xiaohong Teng, and Yongwang Miao
Arch. Anim. Breed., 63, 81–90, https://doi.org/10.5194/aab-63-81-2020, https://doi.org/10.5194/aab-63-81-2020, 2020
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The full CDS of buffalo DGAT2 was isolated and characterized here.
Buffalo DGAT2 contains a conserved domain of DAGAT and a transmembrane region.
It was expressed in the heart, liver, breast, and muscle during lactating and non-lactating stages,
but the expression in the first three tissues of lactation was significantly higher than that of non-lactation.
The results indicate that buffalo DGAT2 may play a role in the endoplasmic reticulum and participate in milk
fat synthesis.
Xinyang Fan, Zifang Zhang, Lihua Qiu, Yongyun Zhang, and Yongwang Miao
Arch. Anim. Breed., 62, 585–596, https://doi.org/10.5194/aab-62-585-2019, https://doi.org/10.5194/aab-62-585-2019, 2019
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Kappa casein (κ-CN) plays an important role in the formation, size and stability of casein micelles. We identified a total of eight single nucleotide polymorphisms in buffalo CSN3. Based on these, seven κ-CN variants and four synonymous variants were inferred and named. The sequences of κ-CN variants were significantly different between buffalo and the Bos genus. This study can help reveal the different physicochemical and processing properties of milk between buffalo and the Bos genus.
Shen Song, Yina Ou-Yang, Jinlong Huo, Yongyun Zhang, Changlin Yu, Minhui Liu, Xiaohong Teng, and Yongwang Miao
Arch. Anim. Breed., 59, 363–372, https://doi.org/10.5194/aab-59-363-2016, https://doi.org/10.5194/aab-59-363-2016, 2016
Shen Song, Jinlong Huo, Feng Yuan, Yina Ou-Yang, DaLin Li, YueYun Yuan, Tao Chen, LianJun Li, and YongWang Miao
Arch. Anim. Breed., 57, 15, https://doi.org/10.7482/0003-9438-57-015, https://doi.org/10.7482/0003-9438-57-015, 2014
Related subject area
Functional genetics
Genetic analysis of platelet-derived growth factor receptor-like gene (PDGFRL) polymorphism and melanin traits in Lanping black-boned sheep (Ovis aries)
Investigating InDels in YAP and TAZ genes and their impact on growth characteristics in goats
The genetic diversity and structure of the Haflinger horse population in the Czech Republic
Evaluation of genetic diversity within different rabbit (Oryctolagus cuniculus) genotypes utilizing start codon targeted (SCoT) and inter-simple sequence repeat (ISSR) molecular markers
Analysis of genetic variants for different horn phenotypes and their inheritance in Icelandic sheep
Myostatin mutation (g+6723G > A) introgression: comparative analysis of growth, slaughter, and carcass traits in Texel, Pırlak, and their crosses (F1 and BC1)
Polymorphisms of the BMPR1B, BMP15 and GDF9 fecundity genes in four Chinese sheep breeds
Polymorphism and expression of GLUD1 in relation to reproductive performance in Jining Grey goats
Milk-y Way: the impact of single-nucleotide polymorphisms on milk production traits in Kilis dairy goats
CircGUCY2C regulates cofilin 1 by sponging miR-425-3p to promote the proliferation of porcine skeletal muscle satellite cells
Novel MHC BLB2 gene polymorphism and its association with IgY concentration and Newcastle disease antibody titer in IPB-D2 chickens
Dan Yue, Chaochao Peng, Sameeullah Memon, Azeem Iqbal, Heli Xiong, Xiaoming He, Ying Lu, and Weidong Deng
Arch. Anim. Breed., 67, 383–392, https://doi.org/10.5194/aab-67-383-2024, https://doi.org/10.5194/aab-67-383-2024, 2024
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We focus on the Lanping black-boned sheep (LPBB) because it is the only mammal that resembles the Chinese silky fowl in the world. LPBB is characterized by black pigmentation in its plasma colorimetry and had its internal organs compared to the reddish color in Lanping normal sheep. Although the literature is abundant in genetic case studies, the processes of pigmentation remain obscure. We thus investigate the association between the PDGFRL gene polymorphism of LPBB and melanin deposition.
Wanxia Zhao, Ziteng Wang, Yichen Lei, Xiaoqin Tang, Xiaohua Yi, Junyi Jiang, Jiapeng Li, Shuhui Wang, and Xiuzhu Sun
Arch. Anim. Breed., 67, 343–351, https://doi.org/10.5194/aab-67-343-2024, https://doi.org/10.5194/aab-67-343-2024, 2024
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In this study, InDels loci of YAP and TAZ genes in two goat breeds were screened and identified, and the association between InDels loci of YAP and TAZ genes and growth traits was analyzed. The results showed that eight InDels loci were identified from goat YAP and TAZ genes. These InDels loci significantly affected growth traits such as chest width, body height, and body weight of goats. These findings can provide new molecular markers for goat molecular breeding.
Michaela Kulišťáková, Iva Jiskrová, Irena Vrtková, Petra Bartoňová, and Tomáš Urban
Arch. Anim. Breed., 67, 323–333, https://doi.org/10.5194/aab-67-323-2024, https://doi.org/10.5194/aab-67-323-2024, 2024
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The aim of this study is to assess the genetic diversity and structure of the Haflinger horse population in the Czech Republic based on 16 microsatellite loci. The results indicated a high level of diversity and the presence of greater admixture, and the identification of distinct genetic clusters suggested a significant gene flow. These findings imply that the population exhibits sufficient genetic variability and diversity.
Nouran Elsayed, Abd Elrahman E. Mandour, Mamdouh K. A. Amin, Fayiz M. Reda, Heba S. A. Taha, Alessandro Di Cerbo, Mahmoud M. Azzam, and Mahmoud Alagawany
Arch. Anim. Breed., 67, 285–295, https://doi.org/10.5194/aab-67-285-2024, https://doi.org/10.5194/aab-67-285-2024, 2024
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It was suggested that the start codon targeted (SCoT) markers may be more effective than inter-simple sequence repeat (ISSR) for differentiating and identifying the genetic variations within investigated breeds. Also, the usage of molecular markers of SCoT and ISSR may be more proper for calculating genetic diversity and common ancestry among tested rabbit breeds.
Rebecca Simon, Karólína Elísabetardóttir, and Gesine Lühken
Arch. Anim. Breed., 67, 237–246, https://doi.org/10.5194/aab-67-237-2024, https://doi.org/10.5194/aab-67-237-2024, 2024
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Genetics of horn traits in sheep are still under investigation. Icelandic sheep were chosen to further characterize this complex trait using known variants as it is highly variable for horn characteristics. Only the published variant for multi-hornedness was in perfect segregation. The absence of horns was analyzed in multi-horned sheep. Only tendencies of association were found for hornlessness and for different horn shapes and sizes, confirming previous results in other breeds.
Serdar Koçak, Mustafa Tekerli, Metin Erdoğan, Koray Çelikeloğlu, Ebubekir Yazıcı, Zehra Bozkurt, Özlem Hacan, Mustafa Demirtaş, and Samet Çinkaya
Arch. Anim. Breed., 67, 207–216, https://doi.org/10.5194/aab-67-207-2024, https://doi.org/10.5194/aab-67-207-2024, 2024
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It was detected that Texel and myostatin-carrying BC1 lambs were significantly higher than Pırlak and non-carrying BC1 lambs in the MLD area at weaning. Myostatin-carrying BC1 lambs had a superiority to Pırlak lambs in terms of muscle conformation and fatness of the hind leg. Myostatin-carrying BC1 lambs were found to be better than Pırlak in growth performance. It was concluded that the Pırlak lambs may be improved by introgression of myostatin mutation.
Jinxin Wang, Yufang Liu, Siwu Guo, Ran Di, Xiangyu Wang, Xiaoyun He, and Mingxing Chu
Arch. Anim. Breed., 67, 51–60, https://doi.org/10.5194/aab-67-51-2024, https://doi.org/10.5194/aab-67-51-2024, 2024
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Finding polymorphisms of genes can provide potential molecular genetic markers for sheep reproduction. This study investigated the polymorphisms of three major reproductive genes (BMPR1B, BMP15 and GDF9) in Chinese sheep and found that BMPR1B and GDF9 might be the major genes influencing the reproductive performance of Chinese sheep breeds. This result might contribute to improving the prolificacy traits of native Chinese sheep in the future.
Wei Wang, Yongjuan Wang, Yufang Liu, Guiling Cao, Ran Di, Jinyu Wang, and Mingxing Chu
Arch. Anim. Breed., 66, 411–419, https://doi.org/10.5194/aab-66-411-2023, https://doi.org/10.5194/aab-66-411-2023, 2023
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In this study, GLUD1 single nucleotide polymorphisms (SNPs) were analyzed in early-puberty goats and delayed-puberty goat breeds by sequencing and restriction fragment length polymorphism (RFLP). The present study aimed to find genetic markers related to sexual precocity and high fertility in goats and to provide a scientific basis for marker-assisted early selection of reproductive performance.
Zühal Gündüz and Osman Biçer
Arch. Anim. Breed., 66, 369–378, https://doi.org/10.5194/aab-66-369-2023, https://doi.org/10.5194/aab-66-369-2023, 2023
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The Kilis goat has emerged as a breed with a unique genetic profile that reflects its complex ancestry due to historical crossbreeding between the Damascus and Hair goat populations in Türkiye. The Kilis goat is well adapted to arid areas and is known for its high milk yield. Therefore, it holds great potential for milk production and genetic improvement programs in Türkiye. For this reason, some genes that affect milk yield were investigated.
Kunlong Qi, Yaqing Dou, Chenlei Li, Yingke Liu, Chenglei Song, Xinjian Li, Kejun Wang, Ruimin Qiao, Xiuling Li, Feng Yang, and Xuelei Han
Arch. Anim. Breed., 66, 285–298, https://doi.org/10.5194/aab-66-285-2023, https://doi.org/10.5194/aab-66-285-2023, 2023
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With the rapid development of sequencing technology, it has been found that circRNAs play an important role in skeletal muscle development. During the growth and development of livestock, the growth and development of muscle fibers are closely related to the number, proliferation, and differentiation capacity of skeletal muscle satellite cells, thereby affecting meat quality. Notably, more and more studies now suggest that changes in circRNA expression may be associated with meat quality.
Dwi Lestari, Sri Murtini, Niken Ulupi, Asep Gunawan, and Cece Sumantri
Arch. Anim. Breed., 66, 275–283, https://doi.org/10.5194/aab-66-275-2023, https://doi.org/10.5194/aab-66-275-2023, 2023
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IPB-D2 chickens are one of the local Indonesian chickens that are being developed today. IPB-D2 chickens have disease-resistant traits. Selection is not only carried out conventionally but is also developed by molecular selection methods. By utilizing gene diversity in the major histocompatibility complex gene group, it was found that there were several single-nucleotide polymorphisms in the B Locus Beta 2 gene significantly associated with immunoglobulin Y concentrations in IPB-D2 chickens.
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Short summary
The aim of this study was to investigate the role of TP53 in buffalo lactation. The data showed that buffalo TP53 inhibited the expression of genes related to milk protein and milk fat in buffalo mammary epithelial cells by inhibiting the PI3K–AKT–mTOR pathway. These results suggest that TP53 negatively regulates the synthesis of milk protein and milk fat in buffalo through the PI3K–AKT–mTOR pathway. This study provides new insights into the functional role of TP53 in buffalo lactation.
The aim of this study was to investigate the role of TP53 in buffalo lactation. The data showed...