Articles | Volume 65, issue 4
https://doi.org/10.5194/aab-65-357-2022
https://doi.org/10.5194/aab-65-357-2022
Original study
 | 
10 Oct 2022
Original study |  | 10 Oct 2022

Bta-miR-33a affects gene expression and lipid levels in Chinese Holstein mammary epithelial cells

Ping Jiang, Ambreen Iqbal, Zhiqian Cui, Haibin Yu, and Zhihui Zhao

Cited articles

Ambros, V.: The functions of animal microRNAs, Nature, 431, 350–355, https://doi.org/10.1038/nature02871, 2004. 
Bartel, D. P.: MicroRNAs: Genomics, Biogenesis, Mechanism, and Function, Cell, 116, 281–297, https://doi.org/10.1016/S0092-8674(04)00045-5, 2004. 
Benatti, R. O., Melo, A. M., Borges, F. O., Ignacio-Souza, L. M., Simino, L. A. P., Milanski, M., Velloso, L. A., Torsoni, M. A., and Torsoni, A. S.: Maternal high-fat diet consumption modulates hepatic lipid metabolism and microRNA-122 (miR-122) and microRNA-370 (miR-370) expression in offspring, Br. J. Nutr., 111, 2112–2122, https://doi.org/10.1017/S0007114514000579, 2014. 
Bond, L. M., Miyazaki, M., O'Neill, L. M., Ding, F., and Ntambi, J. M.: Fatty Acid Desaturation and Elongation in Mammals, in: Biochemistry of Lipids, Lipoproteins and Membranes, 6th Edn., Elsevier, 185–208, https://doi.org/10.1016/B978-0-444-63438-2.00006-7, 2016. 
Dávalos, A., Goedeke, L., Smibert, P., Ramírez, C. M., Warrier, N. P., Andreo, U., Cirera-Salinas, D., Rayner, K., Suresh, U., Pastor-Pareja, J. C., Esplugues, E., Fisher, E. A., Penalva, L. O. F., Moore, K. J., Suárez, Y., Lai, E. C., and Fernández-Hernando, C.: miR-33a/b contribute to the regulation of fatty acid metabolism and insulin signaling, P. Natl. Acad. Sci. USA, 108, 9232–9237, https://doi.org/10.1073/pnas.1102281108, 2011. 
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Short summary
We present a novel study of the effect of bta-miR-33a on lipid metabolism in bovine mammary epithelial cells (BMECs). The correlation between bta-miR-33a and target gene ELOVL6 is also verified by a luciferase reporter assay. We show that bta-miR-33a directly inhibits the expression of ELOVL6 in BMECs, altering the mRNA expression of relative genes. This suggest that bta-miR-33a regulates lipid metabolism by targeting ELOVL6, which might be a potential molecular marker of milk fat composition.
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