Articles | Volume 64, issue 1
Arch. Anim. Breed., 64, 265–271, 2021
https://doi.org/10.5194/aab-64-265-2021
Arch. Anim. Breed., 64, 265–271, 2021
https://doi.org/10.5194/aab-64-265-2021

Original study 16 Jun 2021

Original study | 16 Jun 2021

Improvement of the in vitro fertilization and embryo development using frozen–thawed spermatozoa of microminipigs

Zhao Namula et al.

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Cited articles

Ahmad, Z., Anzar, M., Shahab, M., Ahmad, N., and Andrabi, S. M.: Sephadex and sephadex ion-exchange filtration improves the quality and freezability of low-grade buffalo semen ejaculates, Theriogenology, 59, 1189–1202, 2003. 
Barbas, J. P. and Mascarenhas, R. D.: Cryopreservation of domestic animal sperm cells, Cell Tissue Bank, 10, 49–62, 2009. 
Corcuera, B. D., Marigorta, P., Sagues, A., Saiz-Cidoncha, F., and Perez-Gutierrez, J. F.: Effect of lactose and glycerol on the motility, normal apical ridge, chromatin condensation and chromatin stability of frozen boar spermatozoa, Theriogenology, 67, 1150–1157, 2007. 
Cordova-Izquierdo, A., Oliva, J. H., Lleo, B., Garcia-Artiga, C., Corcuera, B. D., and Perez-Gutierrez, J. F.: Effect of different thawing temperatures on the viability, in vitro fertilizing capacity and chromatin condensation of frozen boar semen packaged in 5 ml straws, Anim. Reprod. Sci., 92, 145–154, 2006. 
Coy, P., Martinez, E., Ruiz, S., Vazquez, J. M., Roca, J., Matas, C., and Pellicer, M. T.: In vitro fertilization of pig oocytes after different coincubation intervals, Theriogenology, 39, 1201–1208, 1993. 
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Short summary
This study aimed to compare the quality of frozen–thawed spermatozoa from microminipig boars. We also evaluated the effects of caffeine and heparin as well as the sperm–oocyte co-incubation length on the fertilization and embryonic development. We found that the presence of caffeine in the in vitro fertilization (IVF) medium and the appropriate length of sperm–oocyte co-incubation may have beneficial effects for improving IVF results when using microminipig spermatozoa with low quality.