Articles | Volume 64, issue 1
Arch. Anim. Breed., 64, 265–271, 2021
Arch. Anim. Breed., 64, 265–271, 2021

Original study 16 Jun 2021

Original study | 16 Jun 2021

Improvement of the in vitro fertilization and embryo development using frozen–thawed spermatozoa of microminipigs

Zhao Namula et al.

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Cited articles

Ahmad, Z., Anzar, M., Shahab, M., Ahmad, N., and Andrabi, S. M.: Sephadex and sephadex ion-exchange filtration improves the quality and freezability of low-grade buffalo semen ejaculates, Theriogenology, 59, 1189–1202, 2003. 
Barbas, J. P. and Mascarenhas, R. D.: Cryopreservation of domestic animal sperm cells, Cell Tissue Bank, 10, 49–62, 2009. 
Corcuera, B. D., Marigorta, P., Sagues, A., Saiz-Cidoncha, F., and Perez-Gutierrez, J. F.: Effect of lactose and glycerol on the motility, normal apical ridge, chromatin condensation and chromatin stability of frozen boar spermatozoa, Theriogenology, 67, 1150–1157, 2007. 
Cordova-Izquierdo, A., Oliva, J. H., Lleo, B., Garcia-Artiga, C., Corcuera, B. D., and Perez-Gutierrez, J. F.: Effect of different thawing temperatures on the viability, in vitro fertilizing capacity and chromatin condensation of frozen boar semen packaged in 5 ml straws, Anim. Reprod. Sci., 92, 145–154, 2006. 
Coy, P., Martinez, E., Ruiz, S., Vazquez, J. M., Roca, J., Matas, C., and Pellicer, M. T.: In vitro fertilization of pig oocytes after different coincubation intervals, Theriogenology, 39, 1201–1208, 1993. 
Short summary
This study aimed to compare the quality of frozen–thawed spermatozoa from microminipig boars. We also evaluated the effects of caffeine and heparin as well as the sperm–oocyte co-incubation length on the fertilization and embryonic development. We found that the presence of caffeine in the in vitro fertilization (IVF) medium and the appropriate length of sperm–oocyte co-incubation may have beneficial effects for improving IVF results when using microminipig spermatozoa with low quality.