Articles | Volume 64, issue 1
Arch. Anim. Breed., 64, 265–271, 2021
https://doi.org/10.5194/aab-64-265-2021
Arch. Anim. Breed., 64, 265–271, 2021
https://doi.org/10.5194/aab-64-265-2021

Original study 16 Jun 2021

Original study | 16 Jun 2021

Improvement of the in vitro fertilization and embryo development using frozen–thawed spermatozoa of microminipigs

Zhao Namula et al.

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Short summary
This study aimed to compare the quality of frozen–thawed spermatozoa from microminipig boars. We also evaluated the effects of caffeine and heparin as well as the sperm–oocyte co-incubation length on the fertilization and embryonic development. We found that the presence of caffeine in the in vitro fertilization (IVF) medium and the appropriate length of sperm–oocyte co-incubation may have beneficial effects for improving IVF results when using microminipig spermatozoa with low quality.