Articles | Volume 62, issue 1
https://doi.org/10.5194/aab-62-125-2019
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/aab-62-125-2019
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Variation in the caprine keratin-associated protein 15-1 (KAP15-1) gene affects cashmere fibre diameter
Mengli Zhao
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of
Animal Science and Technology, Gansu Agricultural University, Lanzhou
730070, China
International Wool Research Institute, Faculty of Animal Science and
Technology, Gansu Agricultural University, Lanzhou 730070, China
Huitong Zhou
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of
Animal Science and Technology, Gansu Agricultural University, Lanzhou
730070, China
International Wool Research Institute, Faculty of Animal Science and
Technology, Gansu Agricultural University, Lanzhou 730070, China
Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences,
Lincoln University, Lincoln 7647, New Zealand
Jon G. H. Hickford
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of
Animal Science and Technology, Gansu Agricultural University, Lanzhou
730070, China
International Wool Research Institute, Faculty of Animal Science and
Technology, Gansu Agricultural University, Lanzhou 730070, China
Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences,
Lincoln University, Lincoln 7647, New Zealand
Hua Gong
International Wool Research Institute, Faculty of Animal Science and
Technology, Gansu Agricultural University, Lanzhou 730070, China
Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences,
Lincoln University, Lincoln 7647, New Zealand
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of
Animal Science and Technology, Gansu Agricultural University, Lanzhou
730070, China
International Wool Research Institute, Faculty of Animal Science and
Technology, Gansu Agricultural University, Lanzhou 730070, China
Jiang Hu
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of
Animal Science and Technology, Gansu Agricultural University, Lanzhou
730070, China
International Wool Research Institute, Faculty of Animal Science and
Technology, Gansu Agricultural University, Lanzhou 730070, China
Xiu Liu
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of
Animal Science and Technology, Gansu Agricultural University, Lanzhou
730070, China
International Wool Research Institute, Faculty of Animal Science and
Technology, Gansu Agricultural University, Lanzhou 730070, China
Shaobin Li
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of
Animal Science and Technology, Gansu Agricultural University, Lanzhou
730070, China
International Wool Research Institute, Faculty of Animal Science and
Technology, Gansu Agricultural University, Lanzhou 730070, China
Zhiyun Hao
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of
Animal Science and Technology, Gansu Agricultural University, Lanzhou
730070, China
International Wool Research Institute, Faculty of Animal Science and
Technology, Gansu Agricultural University, Lanzhou 730070, China
Yuzhu Luo
CORRESPONDING AUTHOR
Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of
Animal Science and Technology, Gansu Agricultural University, Lanzhou
730070, China
International Wool Research Institute, Faculty of Animal Science and
Technology, Gansu Agricultural University, Lanzhou 730070, China
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Yuliang Wen, Jiqing Wang, Xiu Liu, Shaobin Li, Jiang Hu, and Yuzhu Luo
Arch. Anim. Breed., 64, 345–353, https://doi.org/10.5194/aab-64-345-2021, https://doi.org/10.5194/aab-64-345-2021, 2021
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The regulation of glycolysis and heat shock proteins (HSPs) in Gannan yaks at different altitudes were determined. We found that HIF1A and PDK4 expression and glycolysis-related enzyme level were significantly increased, but CA level was significantly decreased with altitude. HSP27 and HSP60 were highly expressed with altitude. These results are useful to better understand the unique adaptability of Gannan yaks, allowing them to survive in hypoxia conditions.
Ishaku Lemu Haruna, Huitong Zhou, and Jon G. H. Hickford
Arch. Anim. Breed., 64, 245–256, https://doi.org/10.5194/aab-64-245-2021, https://doi.org/10.5194/aab-64-245-2021, 2021
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The polymerase chain reaction (PCR) coupled with single-strand conformational polymorphism (SSCP) analysis was used to reveal variation in bovine leptin gene (LEP) in New Zealand (NZ) Holstein Friesian × Jersey (HF × J) dairy cows. This is the first report of findings of this kind in NZ HF × J cows, and they suggest that variation in exon 3 of bovine leptin gene could be explored as a means of decreasing the concentration of saturated fatty acids in milk.
Wenhao Li, Hua Gong, Huitong Zhou, Jiqing Wang, Shaobin Li, Xiu Liu, Yuzhu Luo, and Jon G. H. Hickford
Arch. Anim. Breed., 62, 509–515, https://doi.org/10.5194/aab-62-509-2019, https://doi.org/10.5194/aab-62-509-2019, 2019
Adel H. M. Ibrahim, Nikolaos Tzanidakis, Smaragda Sotiraki, Huitong Zhou, and Jonathan G. H. Hickford
Arch. Anim. Breed., 62, 413–422, https://doi.org/10.5194/aab-62-413-2019, https://doi.org/10.5194/aab-62-413-2019, 2019
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Selective breeding for milk production traits in sheep using molecular marker technology offers a possibility of selecting accurately elite ewes and rams for milk production traits at early age. Our findings suggest that ovine FABP4 has a role in the variation of the milk production traits which may be of economic importance to sheep farmers. Variation in this gene could potentially be used as gene markers for milk production traits if such associations exist.
Related subject area
Subject: DNA markers and gene expressions | Animal: Goats
Overexpression of goat STEAP4 promotes the differentiation of subcutaneous adipocytes
Meta-analysis of association between c.963A>G single-nucleotide polymorphism on BMP15 gene and litter size in goats
TIMP1 may affect goat prolificacy by regulating biological function of granulosa cells
CircRNA-0100 positively regulates the differentiation of cashmere goat SHF-SCs into hair follicle lineage via sequestering miR-153-3p to heighten the KLF5 expression
MiR-25-3p regulates the differentiation of intramuscular preadipocytes in goat via targeting KLF4
EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes
Screening the key genes of hair follicle growth cycle in Inner Mongolian Cashmere goat based on RNA sequencing
A 20-bp insertion/deletion (indel) polymorphism within the CDC25A gene and its associations with growth traits in goat
DNA methylation pattern of the goat PITX1 gene and its effects on milk performance
Genetic characterization of Markhoz goat breed using microsatellite markers
The novel 22 bp insertion mutation in a promoter region of the PITX2 gene is associated with litter size and growth traits in goats
Detection of insertion/deletions (indels) of the ATBF1 gene and their effects on growth-related traits in three indigenous goat breeds
Novel alternative splicing variants of ACOX1 and their differential expression patterns in goats
A novel 12 bp deletion within goat LHX4 gene significantly affected litter size
Xin Li, Hao Zhang, Yong Wang, Yanyan Li, Yan Xiong, Ruiwen Li, Jiangjiang Zhu, and Yaqiu Lin
Arch. Anim. Breed., 65, 397–406, https://doi.org/10.5194/aab-65-397-2022, https://doi.org/10.5194/aab-65-397-2022, 2022
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The effect of STEAP4 (six-transmembrane epithelial antigen of the prostate 4) on goat adipocyte differentiation is unclear. We cloned the goat STEAP4 gene based on the Jianzhou Da’er goat and analyzed its properties and its expression in tissues and adipocytes; further, the positive effect on the expression of subcutaneous adipocytes was obvious. This study will provide a basis for elucidating the biological function of the STEAP4 gene in goat and provide a reference for goat molecular breeding.
Emel Zergani, Amir Rashidi, Jalal Rostamzadeh, Mohammad Razmkabir, and Jens Tetens
Arch. Anim. Breed., 65, 309–318, https://doi.org/10.5194/aab-65-309-2022, https://doi.org/10.5194/aab-65-309-2022, 2022
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Different studies have reported controversial results regarding the effects of c.963A>G polymorphism in the BMP15 gene on litter size in goats. We conducted a meta-analysis by using data of all published relevant studies to address this contradiction and found a significant association between c.963A>G polymorphism and litter size at birth under dominant and additive genetic models. The meta-analysis results revealed that the AA genotype could be positively connected with litter size in goats.
Lei Hong, Xiang Chen, Min Zhu, Zheng Ao, Wen Tang, and Zhinan Zhou
Arch. Anim. Breed., 65, 105–111, https://doi.org/10.5194/aab-65-105-2022, https://doi.org/10.5194/aab-65-105-2022, 2022
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Tissue inhibitor of metalloproteinase 1 (TIMP1) plays a crucial role in follicle growth and embryonic development. Ovarian granulosa cells also play an essential role in these processes. However, the effect of TIMP1 on the biological function of goat ovarian granulosa cells remains unknown. The objective of this study was to explore the impact of TIMP1 on granulosa cell proliferation and reproductive characteristics in goats.
Junyin Zhao, Jincheng Shen, Zeying Wang, Man Bai, Yixing Fan, Yubo Zhu, and Wenlin Bai
Arch. Anim. Breed., 65, 55–67, https://doi.org/10.5194/aab-65-55-2022, https://doi.org/10.5194/aab-65-55-2022, 2022
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Cashmere is a natural fiber from cashmere goats. The textile has been favored by consumers due to typical features like lightness, softness, and comfort. In this study, we show that circRNA-0100 promotes the differentiation of secondary hair follicle stem cells into hair lineage via sequestering miR-153-3p to heighten the KLF5 expression. Our results provide significant information for artificially regulating the secondary hair follicle regeneration and cashmere growth in cashmere goats.
Yu Du, Yue Zhao, Yong Wang, Qingyong Meng, Jiangjiang Zhu, and Yaqiu Lin
Arch. Anim. Breed., 64, 17–25, https://doi.org/10.5194/aab-64-17-2021, https://doi.org/10.5194/aab-64-17-2021, 2021
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Intramuscular fat is a type of fatty tissue deposited between skeletal muscle fibers and muscle bundles, which is regulated by the number and size of preadipocytes in the muscle, and it is a key factor affecting meat tenderness and juiciness. As a member of miRNAs, a variety of studies suggested that miR-25-3p may play an important role in regulating lipid metabolism and adipocyte differentiation. This study attempts to investigate the role of miR-25-3p in goat intramuscular preadipocytes.
Zhihong Wu, Yu Wang, Wenjing Han, Kun Yang, Erhan Hai, Rong Ma, Zhengyang Di, Fangzheng Shang, Rui Su, Ruijun Wang, Zhiying Wang, Yanjun Zhang, and Jinquan Li
Arch. Anim. Breed., 63, 461–470, https://doi.org/10.5194/aab-63-461-2020, https://doi.org/10.5194/aab-63-461-2020, 2020
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This study provided a theoretical and experimental basis for further studying the molecular regulation mechanism of hair follicle development. EDA and EDAR were both expressed in the skin tissue in the seven cashmere goat embryo stages. Moreover, EDA and EDAR play an important role in the formation of embryonic placode (Pc). After interfering with EDA and EDAR, the expression of BMP2, BMP4, noggin, β-catenin, TGF-β2, Wnt-10b, and NOTCH1 in fibroblasts and epithelial cells changed significantly.
Rui Su, Gao Gong, Lingtian Zhang, Xiaochun Yan, Fenghong Wang, Lei Zhang, Xian Qiao, Xiaokai Li, and Jinquan Li
Arch. Anim. Breed., 63, 155–164, https://doi.org/10.5194/aab-63-155-2020, https://doi.org/10.5194/aab-63-155-2020, 2020
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We tried to find important influencing factors of SHF growth cycle in skin tissue from Inner Mongolian Cashmere goats by RNA sequencing (RNA-Seq). Based on the results of comprehensive analysis of differentially expressed genes, GO enrichment and KEGG enrichment, we found that FGF5, FGFR1 and RRAS had an effect on the hair follicle growth cycle. The results of this study may provide a theoretical basis for further research on the growth and development of SHF in Inner Mongolian Cashmere goats.
Wenbo Cui, Nuan Liu, Xuelian Zhang, Yanghai Zhang, Lei Qu, Hailong Yan, Xianyong Lan, Wuzi Dong, and Chuanying Pan
Arch. Anim. Breed., 62, 353–360, https://doi.org/10.5194/aab-62-353-2019, https://doi.org/10.5194/aab-62-353-2019, 2019
Haiyu Zhao, Sihuan Zhang, Xianfeng Wu, Chuanying Pan, Xiangchen Li, Chuzhao Lei, Hong Chen, and Xianyong Lan
Arch. Anim. Breed., 62, 59–68, https://doi.org/10.5194/aab-62-59-2019, https://doi.org/10.5194/aab-62-59-2019, 2019
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This study analyzed the DNA methylation profile of the PITX1 gene and its relevance to lactation performance in goats. The methylation rates of the overall CpG island and the 3rd and 12th CpG-dinucleotide loci in blood were significantly associated with average milk yield. The overall methylation rates of the CpG island in mammary gland tissue from dry and lactation periods showed a significant difference. These results could be used as potential epigenetic markers for lactation performance.
Fariborz Asroush, Seyed-Ziaeddin Mirhoseini, Nejat Badbarin, Alireza Seidavi, Vincenzo Tufarelli, Vito Laudadio, Cataldo Dario, and Maria Selvaggi
Arch. Anim. Breed., 61, 469–473, https://doi.org/10.5194/aab-61-469-2018, https://doi.org/10.5194/aab-61-469-2018, 2018
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The study aimed to genetically characterize Markhoz goat breed using specific microsatellite markers to investigate the genetic diversity within this population and to enhance future breeding strategies and conservation programmes. From findings, despite the decreasing population size, Markhoz goat genetic diversity seems still conserved and the breed seems to have a good level of genetic variability as well as a potential margin of adaptability to environment and future genetic improvement.
Hailong Yan, Enhui Jiang, Haijing Zhu, Linyong Hu, Jinwang Liu, and Lei Qu
Arch. Anim. Breed., 61, 329–336, https://doi.org/10.5194/aab-61-329-2018, https://doi.org/10.5194/aab-61-329-2018, 2018
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As we all know, genes often influence physiological functions of animal organisms such as reproduction, growth, and development. This study explored a novel insertion of paired-like homeodomain 2 (PITX2) gene, and many methods of statistical analysis were used to evaluate their associations with traits of goats. Results indicated that this insertion was significantly associated with litter size, body length, and chest width of goats.
Zhenyu Wei, Ke Wang, Yiqing Hui, Hailong Yan, Haijing Zhu, Lei Qu, Chuanying Pan, Hong Chen, and Xianyong Lan
Arch. Anim. Breed., 61, 311–319, https://doi.org/10.5194/aab-61-311-2018, https://doi.org/10.5194/aab-61-311-2018, 2018
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An effective DNA marker-assisted selection would speed up the development and improvement of goat products. The objective of this work was to explore novel indel (insertion/deletion) variations and their associations with growth traits in three native Chinese goat breeds. Results showed that the goat ATBF1 gene had marked effects on growth traits and the growth-trait-related loci, which would contribute to improving the growth-related traits of local breeds in the goat industry.
Xian-Feng Wu, Yuan Liu, Cheng-Fang Gao, Xin-Zhu Chen, Xiao-Pei Zhang, and Wen-Yang Li
Arch. Anim. Breed., 61, 59–70, https://doi.org/10.5194/aab-61-59-2018, https://doi.org/10.5194/aab-61-59-2018, 2018
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ACOX1 is vital for fatty acid oxidation and deposition. The alternative splicing events of ACOX1 have not been reported in goats. Here, we found a novel splice variant (ACOX1-SV1) and a complete isoform (ACOX1). ACOX1 and ACOX1-SV1 were expressed at high levels in the liver, spleen, brain and fat in kid and adult goats. In both groups, the mRNA level of ACOX1 was high in fat and that of ACOX1-SV1 was high in liver. These findings should provide new insights on the function of ACOX1.
Hailong Yan, Fei Zhang, Ke Wang, Jinwang Liu, Haijing Zhu, Chuanying Pan, and Lei Qu
Arch. Anim. Breed., 61, 1–8, https://doi.org/10.5194/aab-61-1-2018, https://doi.org/10.5194/aab-61-1-2018, 2018
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In this study, a novel 12 bp indel within the LHX4 gene first intron was firstly found. Meanwhile, the association analysis indicated that the 12 bp indel was significantly associated with litter size in 1149 Shaanbei cashmere goats (P < 0.05). The litter sizes of genotype DD and ID individuals were superior to those of genotype II, implying that the 12 bp indel might affect litter size. These results could provide a new insight into implementing marker-assisted selection in goat breeding.
Cited articles
Byun, S. O., Fang, Q., Zhou H., and Hickford, J. G.: An effective method for
silver-staining DNA in large numbers of polyacrylamide gels, Anal. Biochem.,
385, 174–175, https://doi.org/10.1016/j.ab.2008.10.024, 2009.
Gong, H., Zhou, H., McKenzie, G. W., Hickford, J. G. H., Yu, Y., Clerens, S.,
Dyer, J. M., and Plowman, J. E.: Emerging Issues with the Current
Keratin-Associated Protein Nomenclature, Int. J. Trichology, 2, 104–105,
https://doi.org/10.4103/0974-7753.77519, 2010.
Gong, H., Zhou, H., and Hickford J. G.: Diversity of the
glycine/tyrosine-rich keratin-associated protein 6 gene (KAP6) family in
sheep, Mol. Biol. Rep., 38, 31–35, https://doi.org/10.1007/s11033-010-0074-6, 2011.
Gong, H., Zhou, H., McKenzie, G. W., Yu, Z., Clerens, S., Dyer, J. M.,
Plowman, J. E., Wright, M. W., Arora, R., Bawden, C. S., Chen, Y., Li, J.,
and Hickford, J. G. H.: An updated nomenclature for keratin-associated
proteins (KAPs), Int. J. Biol. Sci., 8, 258–264, https://doi.org/10.7150/ijbs.3278,
2012.
Gong, H., Zhou, H., Forrest, R. H., Li, S., Wang, J., Dyer, J. M., Luo, Y.,
and Hickford J. G.: Wool keratin-associated protein genes in sheep-a review,
Genes, 7, 24, https://doi.org/10.3390/genes7060024, 2016.
Jin, M., Wang, L., Li, S., Xing, M. X., and Zhang, X.: Characterization and
expression analysis of KAP7.1, KAP8.2 gene in Liaoning new-breeding cashmere
goat hair follicle, Mol. Biol. Rep., 38, 3023–3028,
https://doi.org/10.1007/s11033-010-9968-6, 2011.
Jin, M., Cao, Q., Wang, R., Piao, J., Zhao, F., and Piao, J.: Molecular
characterization and expression pattern of a novel keratin-associated protein
11.1 gene in the Liaoning cashmere goat (Capra hircus), Asian-Australas. J.
Anim. Sci., 30, 328–337, https://doi.org/10.5713/ajas.16.0078, 2017.
Kijas, J. W., Townley, D., Dalrymple, B. P., Heaton, M. P., Maddox, J. F.,
McGrath, A., Wilson, P., Ingersoll, R. G., McCulloch, R., and McWilliam S.: A
genome wide survey of SNP variation reveals the genetic structure of sheep
breeds, PloS One, 4, e4668, https://doi.org/10.1371/journal.pone.0004668, 2009.
Li, S., Zhou, H., Gong, H., Zhao, F., Hu, J., Luo, Y., and Hickford, J. G.:
Identification of the ovine keratin-associated protein 26-1 gene and its
association with variation in wool traits, Genes, 8, 225,
https://doi.org/10.3390/genes8090225, 2017.
Li, W., Gong, H., Zhou, H., Wang, J., Liu, Xiu., Li, S., Luo, Y., and
Hickford J. G. H.: Variation in the ovine KAP15-1 gene affects wool yield, J.
Agr. Sci., 156, 1–7,
https://doi.org/10.1017/S0021859618000953, 2018.
Liu, H., Li, N., Jia, C., Zhu, X., and Jia, Z.: Effect of the polymorphisms
of keratin associated protein 8.2 gene on fibre traits in Inner Mongolia
cashmere goats, Asian-Australas. J. Anim. Sci., 20, 821–826,
https://doi.org/10.5713/ajas.2007.821, 2007.
Pruett, N. D., Tkatchenko, T. V., Jave-Suarez, L., Jacobs, D. F., Potter, C.
S., Tkatchenko, A. V., Schweizer, J., and Awgulewitsch, A.: Krtap16,
characterization of a new hair keratin-associated protein (KAP) gene complex
on mouse chromosome16 and evidence for regulation by Hoxc13, J. Biol. Chem.,
279, 51524–51533, https://doi.org/10.1074/jbc.M404331200, 2004.
Rogers, G. R., Hickford, J. G. H., and Bickerstaffe, R.: Polymorphism in two
genes for B2 high sulfur proteins of wool, Anim. Genet., 25, 407–415, 1994.
Rogers, M. A., Langbein, L., Winter, H., Ehmann, C., Praetzel, S., and
Schweizer, J.: Characterization of a first domain of human high
glycine-tyrosine and high sulfur keratin-associated protein (KAP) genes on
chromosome 21q22.1, J. Biol. Chem., 277, 48993–49002,
https://doi.org/10.1074/jbc.M206422200, 2002.
Rogers, M. A., Langbein, L., Praetzel-Wunder, S., Winter, H., and Schweizer
J.: Human hair keratin-associated proteins (KAPs), Int. Rev. Cytol., 251,
209–263,
https://doi.org/10.1016/S0074-7696(06)51006-X, 2006.
Wang, J., Che, L., Hickford, J. G. H., Zhou, H., Hao, Z., Luo, Y., Hu,
J., Liu, X., and Li, S.: Identification of the caprine keratin-associated
protein 20-2 (KAP20-2) gene and its effect on cashmere traits, Genes, 8, 328,
https://doi.org/10.3390/genes8110328, 2017.
Wang, J., Hao, Z., Zhou, H., Luo, Y., Hu, J., Liu, X., Li, S., and Hickford
J. G. H.: A keratin-associated protein (KAP) gene that is associated with
variation in cashmere goat fleece weight, Small. Ruminant. Res., 167,
104–109, https://doi.org/10.1016/j.smallrumres.2018.08.014, 2018.
Zhou, H., Hickford, J. G., and Fang, Q.: A two-step procedure for extracting
genomic DNA from dried blood spots on filter paper for polymerase chain
reaction amplification, Anal. Biochem., 354, 159–161,
https://doi.org/10.1016/j.ab.2006.03.042, 2006.
Zhou, H., Gong, H., Yan, W., Luo, Y., and Hickford, J. G.: Identification and
sequence analysis of the keratin-associated protein 24-1 (KAP24-1) gene
homologue in sheep, Gene, 511, 62–65, https://doi.org/10.1016/j.gene.2012.08.049, 2012.
Short summary
Keratin-associated proteins (KAPs) are a structural component of cashmere fibre, and variation in some KAP genes (KRTAPs) has been associated with a number of caprine fibre traits. In this study, we identified the caprine KAP15-1 gene on goat chromosome 1. Six variants containing eight SNPs were found. Association analyses revealed that variation in the gene affected mean fibre diameter.
Keratin-associated proteins (KAPs) are a structural component of cashmere fibre, and variation...