Articles | Volume 61, issue 3
Arch. Anim. Breed., 61, 351–358, 2018
https://doi.org/10.5194/aab-61-351-2018
Arch. Anim. Breed., 61, 351–358, 2018
https://doi.org/10.5194/aab-61-351-2018

Original study 03 Sep 2018

Original study | 03 Sep 2018

Evaluating bovine sperm transfection using a high-performance polymer reagent and assessing the fertilizing capacity of transfected spermatozoa using an in vitro fertilization technique

Ali Jafarnejad et al.

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Subject: Livestock physiology | Animal: Cattle
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Cited articles

Alderson, J., Wilson, B., Laible, G., Pfeffer, P., and L'Huillier, P.: Protamine sulfate protects exogenous DNA against nuclease degradation but is unable to improve the efficiency of bovine sperm mediated transgenesis, Anim. Reprod. Sci., 91, 23–30, 2006. 
Al-Shuhaib, M., Al-Saadi, A., Ewadh, M., and Noor, M.: Sperm Mediated Gene Transfer in Mammals, a Versatile Platform with Multiple Enhancements Techniques, J. Biol. Agric. Healthcare, 4, 58–68, 2014. 
Anzar, M. and Buhr, M. M.: Spontaneous uptake of exogenous DNA by bull spermatozoa, Theriogenology, 65, 683–690, 2006. 
Bachiller, D., Schellander, K., Peli, J., and Ruther, U.: Liposome-mediated DNA uptake by sperm cells, Mol. Reprod. Dev., 30, 194–200, 1991. 
Brackett, B. G., Baranska, W., Sawichi, W., and Koprowski, H.: Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization, P. Natl. Acad. Sci. USA, 68, 353–357, 1971. 
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Short summary
In our study, we attempted to introduce a new device for optimizing sperm-mediated gene transfer. We utilized X-tremeGENE™ HP reagent from the Roche company in bovines, and one-third of total spermatozoa were transfected. We also examined the effect of DMSO on increasing the efficiency of the sperm/DNA transfer to oocytes via IVF. We examined these two reagents in bovine spermatozoa for the first time, which can be considered as the novelty of our work together with its originality.