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Articles | Volume 61, issue 3
Articles | Volume 61, issue 3
https://doi.org/10.5194/aab-61-351-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/aab-61-351-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 61, issue 3
Original study
 | 
03 Sep 2018
Original study |  | 03 Sep 2018

Evaluating bovine sperm transfection using a high-performance polymer reagent and assessing the fertilizing capacity of transfected spermatozoa using an in vitro fertilization technique

Ali Jafarnejad, Mohammad Zandi, Mehdi Aminafshar, Mohammad Reza Sanjabi, and Naser Emamjomeh Kashan

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Latest update: 16 Nov 2024
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Short summary
In our study, we attempted to introduce a new device for optimizing sperm-mediated gene transfer. We utilized X-tremeGENE™ HP reagent from the Roche company in bovines, and one-third of total spermatozoa were transfected. We also examined the effect of DMSO on increasing the efficiency of the sperm/DNA transfer to oocytes via IVF. We examined these two reagents in bovine spermatozoa for the first time, which can be considered as the novelty of our work together with its originality.
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