Excessive amount of lactose in the diet of two-week-old calves induces urinary protein changes
- Department of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, The West Pomeranian University of Technology, Szczecin, Poland
Abstract. The present paper was undertaken to analyse and identify urinary proteins that were significantly altered in urine of calves in response to short-term administration of milk replacer with lactose addition. We used 2-D electrophoresis combined with matrix-assisted laser desorption/ionisation and time-of-flight (MALDI-TOF) mass spectrometry. Of all spots analysed, four showed significantly decreased abundance: alpha-1-antiproteinase (A1AT), serotransferrin (TF), sex hormone-binding globulin (SHBG) and cytochrome P450 2E1 (CYP2E1). One displayed an increased abundance: adenosine triphosphate (ATP)-citrate synthase. The changes in abundance of SHBG and CYP2E1 proteins were caused by the direct effect of an oversupply of sugar, while A1AT, TF and ATP-citrate synthase showed altered abundance probably due to indirect effects. The results of this study confirmed that calves' urine is a very precious biological material to evaluate the renal function, and it may be valuable in veterinary and zootechnical diagnostics.