Articles | Volume 60, issue 4
Original study
17 Oct 2017
Original study |  | 17 Oct 2017

Three-step in vitro maturation culture of bovine oocytes imitating temporal changes of estradiol-17β and progesterone concentrations in preovulatory follicular fluid

Minami Matsuo, Kazuma Sumitomo, Chihiro Ogino, Yosuke Gunji, Ryo Nishimura, and Mitsugu Hishinuma

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Cited articles

Albuz, F. K., Sasseville, M., Lane, M., Armstrong, D. T., Thompson, J. G., and Gilchrist, R. B.: Simulated physiological oocyte maturation (SPOM): a novel in vitro maturation system that substantially improves embryo yield and pregnancy outcomes, Hum. Reprod., 25, 2999–3011,, 2010.
Aparicio, I. M., Garcia-Herreros, M., O'Shea, L. C., Hensey, C., Lonergan, P., and Fair, T.: Expression, regulation, and function of progesterone receptors in bovine cumulus oocyte complexes during in vitro maturation, Biol. Reprod., 84, 910–921,, 2011.
Bain, D. L., Heneghan, A. F., Connaghan-Jones, K. D., and Miura, M. T.: Nuclear receptor structure: implications for function, Annu. Rev. Physiol., 69, 201–220,, 2007.
Beker, A. R., Colenbrander, B., and Bevers, M. M.: Effect of 17β -estradiol on the in vitro maturation of bovine oocytes, Theriogenology, 58, 1663–1673,, 2002.
Beker-van Woudenberg, A. R., van Tol, H. T., Roelen, B. A., Colenbrander, B., and Bevers, M. M.: Estradiol and its membrane-impermeable conjugate (estradiol-bovine serum albumin) during in vitro maturation of bovine oocytes: effects on nuclear and cytoplasmic maturation, cytoskeleton, and embryo quality, Biol. Reprod., 70, 1465–1474,, 2004.
Short summary
Bovine cumulus–oocyte complexes were cultured in a three-step system imitating estradiol-17β (E2) and progesterone (P4) concentrations in preovulatory follicular fluid for in vitro maturation (IVM). The blastocyst formation rate after E2- and P4-imitated IVM was significantly higher than that after the other IVM (control, E2-imitated, P4-imitated), suggesting that the three-step IVM system with the preovulatory levels of E2 and P4 improves the developmental potential of embryos in vitro.