Articles | Volume 60, issue 4
https://doi.org/10.5194/aab-60-385-2017
https://doi.org/10.5194/aab-60-385-2017
Original study
 | 
17 Oct 2017
Original study |  | 17 Oct 2017

Three-step in vitro maturation culture of bovine oocytes imitating temporal changes of estradiol-17β and progesterone concentrations in preovulatory follicular fluid

Minami Matsuo, Kazuma Sumitomo, Chihiro Ogino, Yosuke Gunji, Ryo Nishimura, and Mitsugu Hishinuma

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Latest update: 22 Jun 2024
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Short summary
Bovine cumulus–oocyte complexes were cultured in a three-step system imitating estradiol-17β (E2) and progesterone (P4) concentrations in preovulatory follicular fluid for in vitro maturation (IVM). The blastocyst formation rate after E2- and P4-imitated IVM was significantly higher than that after the other IVM (control, E2-imitated, P4-imitated), suggesting that the three-step IVM system with the preovulatory levels of E2 and P4 improves the developmental potential of embryos in vitro.