Three-step in vitro maturation culture of bovine oocytes imitating temporal changes of estradiol-17β and progesterone concentrations in preovulatory follicular fluid
Laboratory of Theriogenology, Joint Department of Veterinary Medicine,
Faculty of Agriculture, Tottori University, 4-101 Koyama-minami, Tottori
680-8553, Japan
Kazuma Sumitomo
Laboratory of Theriogenology, Joint Department of Veterinary Medicine,
Faculty of Agriculture, Tottori University, 4-101 Koyama-minami, Tottori
680-8553, Japan
Chihiro Ogino
Laboratory of Theriogenology, Joint Department of Veterinary Medicine,
Faculty of Agriculture, Tottori University, 4-101 Koyama-minami, Tottori
680-8553, Japan
Yosuke Gunji
Laboratory of Theriogenology, Joint Department of Veterinary Medicine,
Faculty of Agriculture, Tottori University, 4-101 Koyama-minami, Tottori
680-8553, Japan
Ryo Nishimura
Laboratory of Theriogenology, Joint Department of Veterinary Medicine,
Faculty of Agriculture, Tottori University, 4-101 Koyama-minami, Tottori
680-8553, Japan
Laboratory of Theriogenology, Joint Department of Veterinary Medicine,
Faculty of Agriculture, Tottori University, 4-101 Koyama-minami, Tottori
680-8553, Japan
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Bovine cumulus–oocyte complexes were cultured in a three-step system imitating estradiol-17β (E2) and progesterone (P4) concentrations in preovulatory follicular fluid for in vitro maturation (IVM). The blastocyst formation rate after E2- and P4-imitated IVM was significantly higher than that after the other IVM (control, E2-imitated, P4-imitated), suggesting that the three-step IVM system with the preovulatory levels of E2 and P4 improves the developmental potential of embryos in vitro.
Bovine cumulus–oocyte complexes were cultured in a three-step system imitating estradiol-17β...
