Articles | Volume 68, issue 4
https://doi.org/10.5194/aab-68-703-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/aab-68-703-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Original study
| 
03 Dec 2025
Original study |
| 03 Dec 2025
| 03 Dec 2025
Molecular characterization and functional analysis of buffalo prolactin (PRL) in mammary gland development and lactation
Lige Huang
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China
Animal Genetics and Breeding Institute, Yunnan Agricultural University, Kunming, Yunnan, China
Xinyang Fan
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China
Animal Genetics and Breeding Institute, Yunnan Agricultural University, Kunming, Yunnan, China
Xiaohong Teng
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China
Animal Genetics and Breeding Institute, Yunnan Agricultural University, Kunming, Yunnan, China
Lindong Qian
Faculty of Animal Husbandry and Veterinary Medicine, Yunnan Vocational College of Agriculture, Kunming, Yunnan, China
Zhipeng Bao
CORRESPONDING AUTHOR
Faculty of Animal Husbandry and Veterinary Medicine, Yunnan Vocational College of Agriculture, Kunming, Yunnan, China
Second correspondence author
Yongwang Miao
CORRESPONDING AUTHOR
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China
Animal Genetics and Breeding Institute, Yunnan Agricultural University, Kunming, Yunnan, China
First correspondence author
Related authors
Lige Huang, Dan Sheng, Xinyang Fan, Ruixia Gao, and Yongwang Miao
Arch. Anim. Breed., 67, 217–230, https://doi.org/10.5194/aab-67-217-2024, https://doi.org/10.5194/aab-67-217-2024, 2024
Short summary
Short summary
The aim of this study was to investigate the role of TP53 in buffalo lactation. The data showed that buffalo TP53 inhibited the expression of genes related to milk protein and milk fat in buffalo mammary epithelial cells by inhibiting the PI3K–AKT–mTOR pathway. These results suggest that TP53 negatively regulates the synthesis of milk protein and milk fat in buffalo through the PI3K–AKT–mTOR pathway. This study provides new insights into the functional role of TP53 in buffalo lactation.
Lige Huang, Dan Sheng, Xinyang Fan, Ruixia Gao, and Yongwang Miao
Arch. Anim. Breed., 67, 217–230, https://doi.org/10.5194/aab-67-217-2024, https://doi.org/10.5194/aab-67-217-2024, 2024
Short summary
Short summary
The aim of this study was to investigate the role of TP53 in buffalo lactation. The data showed that buffalo TP53 inhibited the expression of genes related to milk protein and milk fat in buffalo mammary epithelial cells by inhibiting the PI3K–AKT–mTOR pathway. These results suggest that TP53 negatively regulates the synthesis of milk protein and milk fat in buffalo through the PI3K–AKT–mTOR pathway. This study provides new insights into the functional role of TP53 in buffalo lactation.
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Short summary
This study finds that the buffalo prolactin (PRL) gene is structurally similar to that of other Bovidae species and is highly active in the lactating mammary gland. In buffalo mammary epithelial cells (BuMECs), increased PRL stimulated cell proliferation and milk production by activating signaling pathways (Janus kinase 2/signal transducer and activator of transcription 5, mammalian target of papamycin, and mitogen-activated protein kinase). This research provides a basis for improving buffalo milk yield.
This study finds that the buffalo prolactin (PRL) gene is structurally similar to that of other...
