Articles | Volume 62, issue 1
https://doi.org/10.5194/aab-62-59-2019
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/aab-62-59-2019
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Original study
| 
20 Feb 2019
Original study |
| 20 Feb 2019
| 20 Feb 2019
DNA methylation pattern of the goat PITX1 gene and its effects on milk performance
Haiyu Zhao
College of Animal Science and Technology, Northwest A&F
University, Yangling, Shaanxi 712100, China
Sihuan Zhang
College of Animal Science and Technology, Northwest A&F
University, Yangling, Shaanxi 712100, China
Xianfeng Wu
College of Animal Science and Technology, Northwest A&F
University, Yangling, Shaanxi 712100, China
Chuanying Pan
College of Animal Science and Technology, Northwest A&F
University, Yangling, Shaanxi 712100, China
Xiangchen Li
Institute of Beijing Animal Science and Veterinary, Chinese Academy
of Agricultural Science, Beijing 100194, China
Chuzhao Lei
College of Animal Science and Technology, Northwest A&F
University, Yangling, Shaanxi 712100, China
Hong Chen
College of Animal Science and Technology, Northwest A&F
University, Yangling, Shaanxi 712100, China
Xianyong Lan
CORRESPONDING AUTHOR
College of Animal Science and Technology, Northwest A&F
University, Yangling, Shaanxi 712100, China
Related authors
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Zhenyu Lai, Fei Wu, Zihui Zhou, Mei Li, Yuan Gao, Guijun Yin, Jie Yu, Chuzhao Lei, and Ruihua Dang
Arch. Anim. Breed., 63, 377–386, https://doi.org/10.5194/aab-63-377-2020, https://doi.org/10.5194/aab-63-377-2020, 2020
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The purpose of this study was to investigate the expression level and genetic variation of the ACSL1 gene of the Dezhou donkey and its effect on growth traits. The results show that the ACSL1 gene is regularly expressed in Dezhou donkey tissue. Through the association analysis of the genotype and haplotype combination and growth traits, it is speculated that the ACSL1 gene can be used as a candidate gene for Dezhou donkey breeding.
Cui Mao, Xing Ju, Haijian Cheng, Xixia Huang, Fugui Jiang, Yuni Yao, Xianyong Lan, and Enliang Song
Arch. Anim. Breed., 63, 315–323, https://doi.org/10.5194/aab-63-315-2020, https://doi.org/10.5194/aab-63-315-2020, 2020
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To speed up the progress of marker-assisted selection (MAS) in cattle breeding, kinesin family member 1A (KIF1A) genes were chosen based on our previous genome-wide association study (GWAS) analysis results. We detected insertion/deletion (InDel) variation in these three candidate genes in 438 individual cattle (Xinjiang Brown cattle and Wagyu × Luxi crossbreed cattle). These findings may aid future analyses of InDel genotypes in cattle breeds and speed up the progress of MAS in cattle breeding.
Danyang Zhang, Jiawei Xu, Peng Yang, Yifan Wen, Hua He, Jiaxiao Li, Juntong Liang, Yining Zheng, Zijing Zhang, Xianwei Wang, Xiang Yu, Eryao Wang, Chuzhao Lei, Hong Chen, and Yongzhen Huang
Arch. Anim. Breed., 63, 31–37, https://doi.org/10.5194/aab-63-31-2020, https://doi.org/10.5194/aab-63-31-2020, 2020
Mingli Wu, Shipeng Li, Guoliang Zhang, Yingzhi Fan, Yuan Gao, Yongzhen Huang, Xianyong Lan, Chuzhao Lei, Yun Ma, and Ruihua Dang
Arch. Anim. Breed., 62, 465–475, https://doi.org/10.5194/aab-62-465-2019, https://doi.org/10.5194/aab-62-465-2019, 2019
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Four indels were identified by sequencing with DNA pool. Association analysis showed that three of them were associated with growth traits (P<0.05). Our results demonstrated that the polymorphisms in bovine MSRB3 gene were significantly associated with growth traits, which could be candidate loci for marker-assisted selection (MAS) in cattle breeding. These molecular markers are expected to accelerate the process of molecular breeding.
Wenbo Cui, Nuan Liu, Xuelian Zhang, Yanghai Zhang, Lei Qu, Hailong Yan, Xianyong Lan, Wuzi Dong, and Chuanying Pan
Arch. Anim. Breed., 62, 353–360, https://doi.org/10.5194/aab-62-353-2019, https://doi.org/10.5194/aab-62-353-2019, 2019
Hongwei Xu, Xiaoyu Zhang, Rongxin Zang, Yong Cai, Xin Cao, Jutian Yang, Jie Li, Xianyong Lan, and Jianping Wu
Arch. Anim. Breed., 62, 189–197, https://doi.org/10.5194/aab-62-189-2019, https://doi.org/10.5194/aab-62-189-2019, 2019
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In this study, two novel indels within the sheep SIRT7 gene, 5′ promoter region-insertion-7 bp and 3′ UTR-insertion-17 bp, were identified in 709 individuals from five different Chinese and Mongolian sheep breeds. Genetic diversity within the SIRT7 gene and its effect on body size traits were explored. The findings from this research will potentially provide useful DNA markers for the improvement of economic traits in sheep genetic breeding.
Lulan Zeng, Ruihua Dang, Hong Dong, Fangyu Li, Hong Chen, and Chuzhao Lei
Arch. Anim. Breed., 62, 181–187, https://doi.org/10.5194/aab-62-181-2019, https://doi.org/10.5194/aab-62-181-2019, 2019
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Donkeys are an important livestock in China for their nourishment and medical values. Indigenous donkey populations of China retain relatively abundant genetic diversity and Chinese donkeys were grouped into two lineages, which correspond to their geographic distribution and breeding history.
Yi-Lei Ma, Yi-Fan Wen, Xiu-Kai Cao, Jie Cheng, Yong-Zhen Huang, Yun Ma, Lin-Yong Hu, Chu-Zhao Lei, Xing-Lei Qi, Hui Cao, and Hong Chen
Arch. Anim. Breed., 62, 171–179, https://doi.org/10.5194/aab-62-171-2019, https://doi.org/10.5194/aab-62-171-2019, 2019
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We used lots of Chinese cattle to detect the specific regional variation in the IGF1R genome. It was found that this variation in the Chinese cattle population is related to the weight and height of the cattle. There is a lot of genetic information in this mutated region, which may be the cause affecting the traits. Our study provided a preliminary result for the functional role of the IGF1R variation in larger populations and for an important marker in cattle breeding programs.
Zhenyu Wei, Ke Wang, Yiqing Hui, Hailong Yan, Haijing Zhu, Lei Qu, Chuanying Pan, Hong Chen, and Xianyong Lan
Arch. Anim. Breed., 61, 311–319, https://doi.org/10.5194/aab-61-311-2018, https://doi.org/10.5194/aab-61-311-2018, 2018
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An effective DNA marker-assisted selection would speed up the development and improvement of goat products. The objective of this work was to explore novel indel (insertion/deletion) variations and their associations with growth traits in three native Chinese goat breeds. Results showed that the goat ATBF1 gene had marked effects on growth traits and the growth-trait-related loci, which would contribute to improving the growth-related traits of local breeds in the goat industry.
Haoyuan Han, Xin Zhang, Xiaocheng Zhao, Xiaoting Xia, Chuzhao Lei, and Ruihua Dang
Arch. Anim. Breed., 61, 263–270, https://doi.org/10.5194/aab-61-263-2018, https://doi.org/10.5194/aab-61-263-2018, 2018
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The occurrence of copy number variations (CNVs) has been confirmed on the Y
chromosome in horses. However, the copy numbers (CNs) of Equus caballus Y
chromosome (ECAY) genes are largely unknown. This study aimed to demonstrate the copy number variations of Y chromosome genes in horses. Therefore, the quantitative real-time polymerase chain reaction (qPCR) method was applied to measure the CNVs of eight Y chromosome specific genes from 14 different Chinese horse breeds.
Xian-Feng Wu, Yuan Liu, Cheng-Fang Gao, Xin-Zhu Chen, Xiao-Pei Zhang, and Wen-Yang Li
Arch. Anim. Breed., 61, 59–70, https://doi.org/10.5194/aab-61-59-2018, https://doi.org/10.5194/aab-61-59-2018, 2018
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ACOX1 is vital for fatty acid oxidation and deposition. The alternative splicing events of ACOX1 have not been reported in goats. Here, we found a novel splice variant (ACOX1-SV1) and a complete isoform (ACOX1). ACOX1 and ACOX1-SV1 were expressed at high levels in the liver, spleen, brain and fat in kid and adult goats. In both groups, the mRNA level of ACOX1 was high in fat and that of ACOX1-SV1 was high in liver. These findings should provide new insights on the function of ACOX1.
Hailong Yan, Fei Zhang, Ke Wang, Jinwang Liu, Haijing Zhu, Chuanying Pan, and Lei Qu
Arch. Anim. Breed., 61, 1–8, https://doi.org/10.5194/aab-61-1-2018, https://doi.org/10.5194/aab-61-1-2018, 2018
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In this study, a novel 12 bp indel within the LHX4 gene first intron was firstly found. Meanwhile, the association analysis indicated that the 12 bp indel was significantly associated with litter size in 1149 Shaanbei cashmere goats (P < 0.05). The litter sizes of genotype DD and ID individuals were superior to those of genotype II, implying that the 12 bp indel might affect litter size. These results could provide a new insight into implementing marker-assisted selection in goat breeding.
Haoyuan Han, Xiaocheng Zhao, Xiaoting Xia, Hong Chen, Chuzhao Lei, and Ruihua Dang
Arch. Anim. Breed., 60, 391–397, https://doi.org/10.5194/aab-60-391-2017, https://doi.org/10.5194/aab-60-391-2017, 2017
Han Xu, Sihuan Zhang, Xiaoyan Zhang, Ruihua Dang, Chuzhao Lei, Hong Chen, and Xianyong Lan
Arch. Anim. Breed., 60, 285–296, https://doi.org/10.5194/aab-60-285-2017, https://doi.org/10.5194/aab-60-285-2017, 2017
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This work identified SNPs in the growth- and development-related ATBF1 gene in cattle. Five novel SNPs were found in 644 cattle. The results found that SNP1, SNP2, and SNP3 and the combined genotypes SNP1–SNP3, SNP1–SNP4 and SNP2–SNP5 were significantly associated with growth traits in Qinchuan and Jinnan cattle. These findings indicate that the bovine ATBF1 gene has marked effects on growth traits, and the growth-trait-related loci can be used as DNA markers for MAS breeding programs in cattle.
Liangliang Liu, Shuai Yu, Rui Chen, Xiaoyan Lv, and Chuanying Pan
Arch. Anim. Breed., 60, 235–241, https://doi.org/10.5194/aab-60-235-2017, https://doi.org/10.5194/aab-60-235-2017, 2017
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One novel coding SNP was found in exon 1 of pig TMEM95 gene, namely NC_010454.3:g.341T > C, which was significantly associated with testis measurement traits at 15 days of age in the LW population (p < 0.05). Moreover, a significant relationship was found between this A47A polymorphism and testis weight at 40 days of age in LW population (p = 0.047). These results could contribute to breeding and genetics programs in the pig industry via DNA marker-assisted selection (MAS).
Yunyun Jin, Hanfang Cai, Jiming Liu, Fengpeng Lin, Xinglei Qi, Yueyu Bai, Chuzhao Lei, Hong Chen, and Xianyong Lan
Arch. Anim. Breed., 59, 469–476, https://doi.org/10.5194/aab-59-469-2016, https://doi.org/10.5194/aab-59-469-2016, 2016
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Paired box 7 (Pax7) gene, a member of the paired box gene family, plays a critical role in animal growth and muscle development, especially in cell proliferation and self-renewal. In our study, the 10 bp duplication indel was detected in the promoter region within bovine Pax7 gene as well as its association with growth traits. This indel was significantly associated with the body weight in Xianan cattle, the body height in Jinjiang cattle, and the hip width in Pi'nan cattle.
Meng Zhang, Chuanying Pan, Qin Lin, Shenrong Hu, Ruihua Dang, Chuzhao Lei, Hong Chen, and Xianyong Lan
Arch. Anim. Breed., 59, 351–361, https://doi.org/10.5194/aab-59-351-2016, https://doi.org/10.5194/aab-59-351-2016, 2016
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Nanog is an important pluripotent transcription regulator, and its overexpression leads to a high expression of the growth and differentiation factor 3, affecting animal growth traits. The aim of this study was to explore the genetic variations within the Nanog gene and their effects on phenotypic traits in cattle. Six novel exonic single nucleotide polymorphisms were found in six cattle breeds and indicated that Nanog could be a candidate gene for marker-assisted selection in cattle breeding.
Rui Chen, Shuai Yu, Fa Ren, Xiao Yan Lv, and Chuan Ying Pan
Arch. Anim. Breed., 59, 275–283, https://doi.org/10.5194/aab-59-275-2016, https://doi.org/10.5194/aab-59-275-2016, 2016
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Allele "D" (deletion) within the insertion/deletion of SPEF2 gene was dominant in male Large White and Landrace pigs from China. In addition, two novel single-nucleotide polymorphisms of this gene were first identified in pigs. NC_010458:g.19886C > G was significantly associated with testis long circumference (P < 0.05). These findings could not only extend the spectrum of genetic variations of pig SPEF2 gene but also contribute to implementing MAS in pigs.
H. Cai, Z. Wang, X. Lan, Y. Xu, H. Chen, and C. Lei
Arch. Anim. Breed., 59, 91–95, https://doi.org/10.5194/aab-59-91-2016, https://doi.org/10.5194/aab-59-91-2016, 2016
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In this research, the effects of two indel loci of the visfatin gene on mRNA expression levels were studied. The results imply that the expression levels of bovine visfatin vary with age and its indels might be putative variants mediating the expression of the bovine visfatin gene. This study provides useful information for further functional studies of bovine visfatin.
Related subject area
Subject: DNA markers and gene expressions | Animal: Goats
Overexpression of goat STEAP4 promotes the differentiation of subcutaneous adipocytes
Meta-analysis of association between c.963A>G single-nucleotide polymorphism on BMP15 gene and litter size in goats
TIMP1 may affect goat prolificacy by regulating biological function of granulosa cells
CircRNA-0100 positively regulates the differentiation of cashmere goat SHF-SCs into hair follicle lineage via sequestering miR-153-3p to heighten the KLF5 expression
MiR-25-3p regulates the differentiation of intramuscular preadipocytes in goat via targeting KLF4
EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes
Screening the key genes of hair follicle growth cycle in Inner Mongolian Cashmere goat based on RNA sequencing
A 20-bp insertion/deletion (indel) polymorphism within the CDC25A gene and its associations with growth traits in goat
Variation in the caprine keratin-associated protein 15-1 (KAP15-1) gene affects cashmere fibre diameter
Genetic characterization of Markhoz goat breed using microsatellite markers
The novel 22 bp insertion mutation in a promoter region of the PITX2 gene is associated with litter size and growth traits in goats
Detection of insertion/deletions (indels) of the ATBF1 gene and their effects on growth-related traits in three indigenous goat breeds
Novel alternative splicing variants of ACOX1 and their differential expression patterns in goats
A novel 12 bp deletion within goat LHX4 gene significantly affected litter size
Xin Li, Hao Zhang, Yong Wang, Yanyan Li, Yan Xiong, Ruiwen Li, Jiangjiang Zhu, and Yaqiu Lin
Arch. Anim. Breed., 65, 397–406, https://doi.org/10.5194/aab-65-397-2022, https://doi.org/10.5194/aab-65-397-2022, 2022
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The effect of STEAP4 (six-transmembrane epithelial antigen of the prostate 4) on goat adipocyte differentiation is unclear. We cloned the goat STEAP4 gene based on the Jianzhou Da’er goat and analyzed its properties and its expression in tissues and adipocytes; further, the positive effect on the expression of subcutaneous adipocytes was obvious. This study will provide a basis for elucidating the biological function of the STEAP4 gene in goat and provide a reference for goat molecular breeding.
Emel Zergani, Amir Rashidi, Jalal Rostamzadeh, Mohammad Razmkabir, and Jens Tetens
Arch. Anim. Breed., 65, 309–318, https://doi.org/10.5194/aab-65-309-2022, https://doi.org/10.5194/aab-65-309-2022, 2022
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Different studies have reported controversial results regarding the effects of c.963A>G polymorphism in the BMP15 gene on litter size in goats. We conducted a meta-analysis by using data of all published relevant studies to address this contradiction and found a significant association between c.963A>G polymorphism and litter size at birth under dominant and additive genetic models. The meta-analysis results revealed that the AA genotype could be positively connected with litter size in goats.
Lei Hong, Xiang Chen, Min Zhu, Zheng Ao, Wen Tang, and Zhinan Zhou
Arch. Anim. Breed., 65, 105–111, https://doi.org/10.5194/aab-65-105-2022, https://doi.org/10.5194/aab-65-105-2022, 2022
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Tissue inhibitor of metalloproteinase 1 (TIMP1) plays a crucial role in follicle growth and embryonic development. Ovarian granulosa cells also play an essential role in these processes. However, the effect of TIMP1 on the biological function of goat ovarian granulosa cells remains unknown. The objective of this study was to explore the impact of TIMP1 on granulosa cell proliferation and reproductive characteristics in goats.
Junyin Zhao, Jincheng Shen, Zeying Wang, Man Bai, Yixing Fan, Yubo Zhu, and Wenlin Bai
Arch. Anim. Breed., 65, 55–67, https://doi.org/10.5194/aab-65-55-2022, https://doi.org/10.5194/aab-65-55-2022, 2022
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Cashmere is a natural fiber from cashmere goats. The textile has been favored by consumers due to typical features like lightness, softness, and comfort. In this study, we show that circRNA-0100 promotes the differentiation of secondary hair follicle stem cells into hair lineage via sequestering miR-153-3p to heighten the KLF5 expression. Our results provide significant information for artificially regulating the secondary hair follicle regeneration and cashmere growth in cashmere goats.
Yu Du, Yue Zhao, Yong Wang, Qingyong Meng, Jiangjiang Zhu, and Yaqiu Lin
Arch. Anim. Breed., 64, 17–25, https://doi.org/10.5194/aab-64-17-2021, https://doi.org/10.5194/aab-64-17-2021, 2021
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Intramuscular fat is a type of fatty tissue deposited between skeletal muscle fibers and muscle bundles, which is regulated by the number and size of preadipocytes in the muscle, and it is a key factor affecting meat tenderness and juiciness. As a member of miRNAs, a variety of studies suggested that miR-25-3p may play an important role in regulating lipid metabolism and adipocyte differentiation. This study attempts to investigate the role of miR-25-3p in goat intramuscular preadipocytes.
Zhihong Wu, Yu Wang, Wenjing Han, Kun Yang, Erhan Hai, Rong Ma, Zhengyang Di, Fangzheng Shang, Rui Su, Ruijun Wang, Zhiying Wang, Yanjun Zhang, and Jinquan Li
Arch. Anim. Breed., 63, 461–470, https://doi.org/10.5194/aab-63-461-2020, https://doi.org/10.5194/aab-63-461-2020, 2020
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This study provided a theoretical and experimental basis for further studying the molecular regulation mechanism of hair follicle development. EDA and EDAR were both expressed in the skin tissue in the seven cashmere goat embryo stages. Moreover, EDA and EDAR play an important role in the formation of embryonic placode (Pc). After interfering with EDA and EDAR, the expression of BMP2, BMP4, noggin, β-catenin, TGF-β2, Wnt-10b, and NOTCH1 in fibroblasts and epithelial cells changed significantly.
Rui Su, Gao Gong, Lingtian Zhang, Xiaochun Yan, Fenghong Wang, Lei Zhang, Xian Qiao, Xiaokai Li, and Jinquan Li
Arch. Anim. Breed., 63, 155–164, https://doi.org/10.5194/aab-63-155-2020, https://doi.org/10.5194/aab-63-155-2020, 2020
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We tried to find important influencing factors of SHF growth cycle in skin tissue from Inner Mongolian Cashmere goats by RNA sequencing (RNA-Seq). Based on the results of comprehensive analysis of differentially expressed genes, GO enrichment and KEGG enrichment, we found that FGF5, FGFR1 and RRAS had an effect on the hair follicle growth cycle. The results of this study may provide a theoretical basis for further research on the growth and development of SHF in Inner Mongolian Cashmere goats.
Wenbo Cui, Nuan Liu, Xuelian Zhang, Yanghai Zhang, Lei Qu, Hailong Yan, Xianyong Lan, Wuzi Dong, and Chuanying Pan
Arch. Anim. Breed., 62, 353–360, https://doi.org/10.5194/aab-62-353-2019, https://doi.org/10.5194/aab-62-353-2019, 2019
Mengli Zhao, Huitong Zhou, Jon G. H. Hickford, Hua Gong, Jiqing Wang, Jiang Hu, Xiu Liu, Shaobin Li, Zhiyun Hao, and Yuzhu Luo
Arch. Anim. Breed., 62, 125–133, https://doi.org/10.5194/aab-62-125-2019, https://doi.org/10.5194/aab-62-125-2019, 2019
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Keratin-associated proteins (KAPs) are a structural component of cashmere fibre, and variation in some KAP genes (KRTAPs) has been associated with a number of caprine fibre traits. In this study, we identified the caprine KAP15-1 gene on goat chromosome 1. Six variants containing eight SNPs were found. Association analyses revealed that variation in the gene affected mean fibre diameter.
Fariborz Asroush, Seyed-Ziaeddin Mirhoseini, Nejat Badbarin, Alireza Seidavi, Vincenzo Tufarelli, Vito Laudadio, Cataldo Dario, and Maria Selvaggi
Arch. Anim. Breed., 61, 469–473, https://doi.org/10.5194/aab-61-469-2018, https://doi.org/10.5194/aab-61-469-2018, 2018
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The study aimed to genetically characterize Markhoz goat breed using specific microsatellite markers to investigate the genetic diversity within this population and to enhance future breeding strategies and conservation programmes. From findings, despite the decreasing population size, Markhoz goat genetic diversity seems still conserved and the breed seems to have a good level of genetic variability as well as a potential margin of adaptability to environment and future genetic improvement.
Hailong Yan, Enhui Jiang, Haijing Zhu, Linyong Hu, Jinwang Liu, and Lei Qu
Arch. Anim. Breed., 61, 329–336, https://doi.org/10.5194/aab-61-329-2018, https://doi.org/10.5194/aab-61-329-2018, 2018
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As we all know, genes often influence physiological functions of animal organisms such as reproduction, growth, and development. This study explored a novel insertion of paired-like homeodomain 2 (PITX2) gene, and many methods of statistical analysis were used to evaluate their associations with traits of goats. Results indicated that this insertion was significantly associated with litter size, body length, and chest width of goats.
Zhenyu Wei, Ke Wang, Yiqing Hui, Hailong Yan, Haijing Zhu, Lei Qu, Chuanying Pan, Hong Chen, and Xianyong Lan
Arch. Anim. Breed., 61, 311–319, https://doi.org/10.5194/aab-61-311-2018, https://doi.org/10.5194/aab-61-311-2018, 2018
Short summary
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An effective DNA marker-assisted selection would speed up the development and improvement of goat products. The objective of this work was to explore novel indel (insertion/deletion) variations and their associations with growth traits in three native Chinese goat breeds. Results showed that the goat ATBF1 gene had marked effects on growth traits and the growth-trait-related loci, which would contribute to improving the growth-related traits of local breeds in the goat industry.
Xian-Feng Wu, Yuan Liu, Cheng-Fang Gao, Xin-Zhu Chen, Xiao-Pei Zhang, and Wen-Yang Li
Arch. Anim. Breed., 61, 59–70, https://doi.org/10.5194/aab-61-59-2018, https://doi.org/10.5194/aab-61-59-2018, 2018
Short summary
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ACOX1 is vital for fatty acid oxidation and deposition. The alternative splicing events of ACOX1 have not been reported in goats. Here, we found a novel splice variant (ACOX1-SV1) and a complete isoform (ACOX1). ACOX1 and ACOX1-SV1 were expressed at high levels in the liver, spleen, brain and fat in kid and adult goats. In both groups, the mRNA level of ACOX1 was high in fat and that of ACOX1-SV1 was high in liver. These findings should provide new insights on the function of ACOX1.
Hailong Yan, Fei Zhang, Ke Wang, Jinwang Liu, Haijing Zhu, Chuanying Pan, and Lei Qu
Arch. Anim. Breed., 61, 1–8, https://doi.org/10.5194/aab-61-1-2018, https://doi.org/10.5194/aab-61-1-2018, 2018
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In this study, a novel 12 bp indel within the LHX4 gene first intron was firstly found. Meanwhile, the association analysis indicated that the 12 bp indel was significantly associated with litter size in 1149 Shaanbei cashmere goats (P < 0.05). The litter sizes of genotype DD and ID individuals were superior to those of genotype II, implying that the 12 bp indel might affect litter size. These results could provide a new insight into implementing marker-assisted selection in goat breeding.
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Short summary
This study analyzed the DNA methylation profile of the PITX1 gene and its relevance to lactation performance in goats. The methylation rates of the overall CpG island and the 3rd and 12th CpG-dinucleotide loci in blood were significantly associated with average milk yield. The overall methylation rates of the CpG island in mammary gland tissue from dry and lactation periods showed a significant difference. These results could be used as potential epigenetic markers for lactation performance.
This study analyzed the DNA methylation profile of the PITX1 gene and its relevance to lactation...
