Articles | Volume 66, issue 3
https://doi.org/10.5194/aab-66-217-2023
https://doi.org/10.5194/aab-66-217-2023
Original study
 | 
28 Jul 2023
Original study |  | 28 Jul 2023

Lin28B overexpression decreases let-7b and let-7g levels and increases proliferation and estrogen secretion in Dolang sheep ovarian granulosa cells

Zhiyuan Sui, Yongjie Zhang, Zhishuai Zhang, Chenguang Wang, Xiaojun Li, and Feng Xing

Related authors

Expression and functional analysis of GnRH at the onset of puberty in sheep
Jihu Zhang, Chenguang Wang, Xiaojun Li, Yongjie Zhang, and Feng Xing
Arch. Anim. Breed., 65, 249–257, https://doi.org/10.5194/aab-65-249-2022,https://doi.org/10.5194/aab-65-249-2022, 2022
Short summary

Cited articles

Ambros, V.: A hierarchy of regulatory genes controls a larva-to-adult developmental switch in C. elegans, Cell, 57, 49–57, https://doi.org/10.1016/0092-8674(89)90171-2, 1989. 
Baek, D., Villen, J., Shin, C., Camargo, F. D., Gygi, S. P., and Bartel, D. P.: The impact of microRNAs on protein output, Nature, 455, 64–71, https://doi.org/10.1038/nature07242, 2008. 
Bartel, D. P.: MicroRNAs: genomics, biogenesis, mechanism, and function, Cell, 116, 281–297, https://doi.org/10.1016/s0092-8674(04)00045-5, 2004. 
Cao, G., Gao, Z., Jiang, Y., and Chu, M.: Lin28 gene and mammalian puberty, Mol. Reprod. Dev., 87, 525–533, https://doi.org/10.1002/mrd.23347, 2020. 
Chou, C. H. and Chen, M. J.: The Effect of Steroid Hormones on Ovarian Follicle Development, Vitam. Horm., 107, 155–175, https://doi.org/10.1016/bs.vh.2018.01.013, 2018. 
Download
Short summary
There are few studies investigating the RNA-binding protein Lin28B and ovine puberty. This study focuses on understanding how its overexpression changes microRNA levels, cell proliferation, and estrogen secretion. To assess these changes, we used quantitative real-time (qRT) PCR, overexpression vectors, and ELISA. We found that Lin28B overexpression decreases let-7b and let-7g levels and increases granulosa cell proliferation and their estrogen secretion.