Articles | Volume 66, issue 3
https://doi.org/10.5194/aab-66-217-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/aab-66-217-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Lin28B overexpression decreases let-7b and let-7g levels and increases proliferation and estrogen secretion in Dolang sheep ovarian granulosa cells
Zhiyuan Sui
Key Laboratory of Tarim, Animal Husbandry Science and Technology,
Xinjiang Production & Construction Corps, Alar, Xinjiang 843300, China
College of Animal Science and Technology, Tarim University, Alar,
Xinjiang 843300, China
Yongjie Zhang
Key Laboratory of Tarim, Animal Husbandry Science and Technology,
Xinjiang Production & Construction Corps, Alar, Xinjiang 843300, China
College of Animal Science and Technology, Tarim University, Alar,
Xinjiang 843300, China
Zhishuai Zhang
Key Laboratory of Tarim, Animal Husbandry Science and Technology,
Xinjiang Production & Construction Corps, Alar, Xinjiang 843300, China
College of Animal Science and Technology, Tarim University, Alar,
Xinjiang 843300, China
Chenguang Wang
Key Laboratory of Tarim, Animal Husbandry Science and Technology,
Xinjiang Production & Construction Corps, Alar, Xinjiang 843300, China
College of Animal Science and Technology, Tarim University, Alar,
Xinjiang 843300, China
Xiaojun Li
Key Laboratory of Tarim, Animal Husbandry Science and Technology,
Xinjiang Production & Construction Corps, Alar, Xinjiang 843300, China
College of Animal Science and Technology, Tarim University, Alar,
Xinjiang 843300, China
Feng Xing
CORRESPONDING AUTHOR
Key Laboratory of Tarim, Animal Husbandry Science and Technology,
Xinjiang Production & Construction Corps, Alar, Xinjiang 843300, China
College of Animal Science and Technology, Tarim University, Alar,
Xinjiang 843300, China
Related authors
No articles found.
Jihu Zhang, Chenguang Wang, Xiaojun Li, Yongjie Zhang, and Feng Xing
Arch. Anim. Breed., 65, 249–257, https://doi.org/10.5194/aab-65-249-2022, https://doi.org/10.5194/aab-65-249-2022, 2022
Short summary
Short summary
This study investigated the coding sequence (CDS) of the GnRH gene in the ovary of Duolang sheep and the expression of GnRH mRNA in different tissues at the onset of puberty and analyzed the effect of GnRH agonists on ovarian granulosa cells (GCs) of Duolang sheep. The role of GnRH in sheep ovaries at the onset of puberty was elucidated.
Cited articles
Ambros, V.: A hierarchy of regulatory genes controls a larva-to-adult
developmental switch in C. elegans, Cell, 57, 49–57,
https://doi.org/10.1016/0092-8674(89)90171-2, 1989.
Baek, D., Villen, J., Shin, C., Camargo, F. D., Gygi, S. P., and Bartel, D.
P.: The impact of microRNAs on protein output, Nature, 455, 64–71,
https://doi.org/10.1038/nature07242, 2008.
Bartel, D. P.: MicroRNAs: genomics, biogenesis, mechanism, and function,
Cell, 116, 281–297, https://doi.org/10.1016/s0092-8674(04)00045-5, 2004.
Cao, G., Gao, Z., Jiang, Y., and Chu, M.: Lin28 gene and mammalian puberty,
Mol. Reprod. Dev., 87, 525–533, https://doi.org/10.1002/mrd.23347, 2020.
Chou, C. H. and Chen, M. J.: The Effect of Steroid Hormones on Ovarian
Follicle Development, Vitam. Horm., 107, 155–175,
https://doi.org/10.1016/bs.vh.2018.01.013, 2018.
Deng, H. X., Yu, Y. Y., Zhou, A. Q., Zhu, J. L., Luo, L. N., Chen, W. Q.,
Hu, L., and Chen, G. X.: Yangzheng Sanjie decoction regulates proliferation
and apoptosis of gastric cancer cells by enhancing let-7a expression, World
J. Gastroentero., 23, 5538–5548, https://doi.org/10.3748/wjg.v23.i30.5538,
2017.
Douville, G. and Sirard, M. A.: Changes in granulosa cells gene expression
associated with growth, plateau and atretic phases in medium bovine
follicles, J. Ovarian. Res., 7, 50, https://doi.org/10.1186/1757-2215-7-50,
2014.
Greives, T. J., Mason, A. O., Scotti, M. A., Levine, J., Ketterson, E. D.,
Kriegsfeld, L. J., and Demas, G. E.: Environmental control of kisspeptin:
implications for seasonal reproduction, Endocrinology, 148, 1158–1166,
https://doi.org/10.1210/en.2006-1249, 2007.
Guo, M., Zhao, X., Yuan, X., Jiang, J., and Li, P.: MiR-let-7a inhibits cell
proliferation, migration, and invasion by down-regulating PKM2 in cervical
cancer, Oncotarget, 8, 28226–28236,
https://doi.org/10.18632/oncotarget.15999, 2017.
Guo, Y., Chen, Y., Ito, H., Watanabe, A., Ge, X., Kodama, T., and Aburatani,
H.: Identification and characterization of lin-28 homolog B (LIN28B) in
human hepatocellular carcinoma, Gene, 384, 51–61,
https://doi.org/10.1016/j.gene.2006.07.011, 2006.
Hanna, J., Saha, K., Pando, B., Van Zon, J., Lengner, C. J., Creyghton, M.
P., van Oudenaarden, A., and Jaenisch, R.: Direct cell reprogramming is a
stochastic process amenable to acceleration, Nature, 462, 595–601,
https://doi.org/10.1038/nature08592, 2009.
Hong, L., Chen, X., Zhu, M., Ao, Z., Tang, W., and Zhou, Z.: TIMP1 may affect goat prolificacy by regulating biological function of granulosa cells, Arch. Anim. Breed., 65, 105–111, https://doi.org/10.5194/aab-65-105-2022, 2022.
Huang, J. H., Duan, H., Wang, S., and Wang, Y. Y.: Estrogen 17betaestradiol
accelerates the proliferation of uterine junctional zone smooth muscle cells
via the let7a/Lin28B axis in adenomyosis, Mol. Med. Rep., 23, 337,
https://doi.org/10.3892/mmr.2021.11976, 2021.
Hwang, H. W. and Mendell, J. T.: MicroRNAs in cell proliferation, cell
death, and tumorigenesis, Br. J. Cancer, 94, 776–780,
https://doi.org/10.1038/sj.bjc.6603023, 2006.
Khan, M. I., Dias, F. C., Dufort, I., Misra, V., Sirard, M. A., and Singh,
J.: Stable reference genes in granulosa cells of bovine dominant follicles
during follicular growth, FSH stimulation and maternal aging, Reprod. Fert.
Develop., 28, 795–805, https://doi.org/10.1071/RD14089, 2016.
Lv, X., He, C., Huang, C., Wang, H., Hua, G., Wang, Z., Zhou, J., Chen, X.,
Ma, B., and Timm, B. K.: Timely expression and activation of YAP1 in
granulosa cells is essential for ovarian follicle development, FASEB
J., 33, 10049–10064, https://doi.org/10.1096/fj.201900179RR, 2019.
Meeran, D., Urbanski, H. F., Gregory, S. J., Townsend, J., and Tortonese, D.
J.: Developmental changes in the hormonal identity of gonadotroph cells in
the rhesus monkey pituitary gland, J. Clin. Endocrinol. Metab., 88, 2934–2942,
https://doi.org/10.1210/jc.2002-021001, 2003.
Molenaar, J. J., Domingo-Fernandez, R., Ebus, M. E., Lindner, S., Koster,
J., Drabek, K., Mestdagh, P., van Sluis, P., Valentijn, L. J., van Nes, J.,
Broekmans, M., Haneveld, F., Volckmann, R., Bray, I., Heukamp, L., Sprussel,
A., Thor, T., Kieckbusch, K., Klein-Hitpass, L., Fischer, M., Vandesompele,
J., Schramm, A., van Noesel, M. M., Varesio, L., Speleman, F., Eggert, A.,
Stallings, R. L., Caron, H. N., Versteeg, R., and Schulte, J. H.: LIN28B
induces neuroblastoma and enhances MYCN levels via let-7 suppression, Nat.
Genet., 44, 1199–1206, https://doi.org/10.1038/ng.2436, 2012.
Murray, M. J., Saini, H. K., Siegler, C. A., Hanning, J. E., Barker, E. M.,
van Dongen, S., Ward, D. M., Raby, K. L., Groves, I. J., and Scarpini, C.
G.: LIN28 Expression in Malignant Germ Cell Tumors Downregulates let-7 and
Increases Oncogene LevelsLIN28/let-7 Deregulation in Malignant Germ Cell
Tumors, Cancer Res., 73, 4872–4884,
https://doi.org/10.1158/0008-5472.CAN-12-2085, 2013.
Peng, S., Chen, L.-L., Lei, X.-X., Yang, L., Lin, H., Carmichael, G. G., and
Huang, Y.: Genome-wide studies reveal that Lin28 enhances the translation of
genes important for growth and survival of human embryonic stem cells, Stem
Cells, 29, 496–504, https://doi.org/10.1002/stem.591, 2011.
Pool, K. R., Rickard, J. P., and de Graaf, S. P.: Overcoming neuroendocrine
and metabolic barriers to puberty: the role of melatonin in advancing
puberty in ewe lambs, Domest. Anim. Endocrin., 72, 106457,
https://doi.org/10.1016/j.domaniend.2020.106457, 2020.
Qi, L., Liu, F., Zhang, F., Zhang, S., Lv, L., Bi, Y., and Yu, Y.: lncRNA
NEAT1 competes against let-7a to contribute to non-small cell lung cancer
proliferation and metastasis, Biomed. Pharmacother., 103, 1507–1515,
https://doi.org/10.1016/j.biopha.2018.04.053, 2018.
Qian, P., Zuo, Z., Wu, Z., Meng, X., Li, G., Wu, Z., Zhang, W., Tan, S.,
Pandey, V., Yao, Y., Wang, P., Zhao, L., Wang, J., Wu, Q., Song, E., Lobie,
P. E., Yin, Z., and Zhu, T.: Pivotal role of reduced let-7g expression in
breast cancer invasion and metastasis, Cancer Res., 71, 6463–6474,
https://doi.org/10.1158/0008-5472.CAN-11-1322, 2011.
Redmond, J. S., Macedo, G. G., Velez, I. C., Caraty, A., Williams, G. L.,
and Amstalden, M.: Kisspeptin activates the
hypothalamic-adenohypophyseal-gonadal axis in prepubertal ewe lambs,
Reproduction, 141, 541–548, https://doi.org/10.1530/REP-10-0467, 2011.
Rosa, H. J. and Bryant, M. J.: Seasonality of reproduction in sheep, Small
Ruminant Res., 48, 155–171,
https://doi.org/10.1016/S0921-4488(03)00038-5, 2003.
Saatcioglu, H. D., Cuevas, I., and Castrillon, D. H.: Control of Oocyte
Reawakening by Kit, PLoS Genet, 12, e1006215,
https://doi.org/10.1371/journal.pgen.1006215, 2016.
Sangiao-Alvarellos, S., Manfredi-Lozano, M., Ruiz-Pino, F., Navarro, V. M.,
Sanchez-Garrido, M. A., Leon, S., Dieguez, C., Cordido, F., Matagne, V.,
Dissen, G. A., Ojeda, S. R., Pinilla, L., and Tena-Sempere, M.: Changes in
hypothalamic expression of the Lin28/let-7 system and related microRNAs
during postnatal maturation and after experimental manipulations of puberty,
Endocrinology, 154, 942–955, https://doi.org/10.1210/en.2012-2006, 2013.
Schultz, J., Lorenz, P., Gross, G., Ibrahim, S., and Kunz, M.: MicroRNA
let-7b targets important cell cycle molecules in malignant melanoma cells
and interferes with anchorage-independent growth, Cell Res., 18, 549–557,
https://doi.org/10.1038/cr.2008.45, 2008.
Shao, Y., Zhang, L., Cui, L., Lou, W., Wang, D., Lu, W., Jin, D., and Liu, T.: LIN28B suppresses microRNA let-7b expression to promote CD44+/LIN28B+ human pancreatic cancer stem cell proliferation and invasion, Am. J. Cancer Res., 5, 2643–2659, PMID: 26609473; PMCID: PMC4633895, 2015.
Shyh-Chang, N. and Daley, G. Q.: Lin28: primal regulator of growth and
metabolism in stem cells, Cell Stem. Cell, 12, 395–406,
https://doi.org/10.1016/j.stem.2013.03.005, 2013.
Sun, X., Qin, S., Fan, C., Xu, C., Du, N., and Ren, H.: Let-7: a regulator
of the ERalpha signaling pathway in human breast tumors and breast cancer
stem cells, Oncol. Rep., 29, 2079–2087, https://doi.org/10.3892/or.2013.2330,
2013.
Sun, X., Xu, C., Tang, S. C., Wang, J., Wang, H., Wang, P., Du, N., Qin, S.,
Li, G., Xu, S., Tao, Z., Liu, D., and Ren, H.: Let-7c blocks
estrogen-activated Wnt signaling in induction of self-renewal of breast
cancer stem cells, Cancer Gene Ther., 23, 83–89,
https://doi.org/10.1038/cgt.2016.3, 2016.
Suttie, J. M., Gluckman, P. D., Butler, J. H., Fennessy, P. F., Corson, I.
D., and Laas, F. J.: Insulin-like growth factor 1 (IGF-1) antler-stimulating
hormone?, Endocrinology, 116, 846–848,
https://doi.org/10.1210/endo-116-2-846, 1985.
Tommiska, J., Wehkalampi, K., Vaaralahti, K., Laitinen, E. M., Raivio, T.,
and Dunkel, L.: LIN28B in constitutional delay of growth and puberty, J. Clin.
Endocr. Metab., 95, 3063–3066, https://doi.org/10.1210/jc.2009-2344, 2010.
Wang, Y., Duan, C., Guo, Y., Li, J., He, H., Li, R., Zhang, Y., and Liu, Y.:
Effects of glucose on glycolysis and steroidogenesis as well as related gene
expression in ovine granulosa cells in vitro, Small Ruminant Res., 215,
106766, https://doi.org/10.1016/j.smallrumres.2022.106766, 2022.
Wankowska, M., Romanowicz, K., and Polkowska, J.: The neuroendocrine events
during the ovine growth-promoted maturation: the developmental importance of
hypophysiotrophic action of somatostatin in ewes, Anim. Reprod. Sci., 109,
146–160, https://doi.org/10.1016/j.anireprosci.2007.12.007, 2008.
Wu, J., Feng, X., Du, Y., Luan, B., Yu, H., Yu, Y., Wu, L., and Zhao, H.:
beta-catenin/LIN28B promotes the proliferation of human choriocarcinoma
cells via Let-7a repression, Acta Biochim. Biophys. Sin. (Shanghai), 51,
455–462, https://doi.org/10.1093/abbs/gmz027, 2019.
Xing, F., Zhang, C., and Kong, Z.: Cloning and expression of lin-28 homolog
B gene in the onset of puberty in Duolang sheep, Asian-Austral. J. Anim.,
32, 23–30, https://doi.org/10.5713/ajas.18.0276, 2019a.
Xing, F., Zhang, C., and Kong, Z.: Cloning and expression of lin-28 homolog
B gene in the onset of puberty in Duolang sheep, Asian-Austral. J. Anim.,
32, 23–30, https://doi.org/10.5713/ajas.18.0276, 2019b.
Xu, B., Zhang, K., and Huang, Y.: Lin28 modulates cell growth and associates
with a subset of cell cycle regulator mRNAs in mouse embryonic stem cells,
RNA, 15, 357–361, https://doi.org/10.1261/rna.1368009, 2009.
Yu, F., Yao, H., Zhu, P., Zhang, X., Pan, Q., Gong, C., Huang, Y., Hu, X.,
Su, F., Lieberman, J., and Song, E.: let-7 regulates self renewal and
tumorigenicity of breast cancer cells, Cell, 131, 1109–1123,
https://doi.org/10.1016/j.cell.2007.10.054, 2007.
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.
L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., Slukvin,
I. I., and Thomson, J. A.: Induced pluripotent stem cell lines derived from
human somatic cells, Science, 318, 1917–1920,
https://doi.org/10.1126/science.1151526, 2007.
Short summary
There are few studies investigating the RNA-binding protein Lin28B and ovine puberty. This study focuses on understanding how its overexpression changes microRNA levels, cell proliferation, and estrogen secretion. To assess these changes, we used quantitative real-time (qRT) PCR, overexpression vectors, and ELISA. We found that Lin28B overexpression decreases let-7b and let-7g levels and increases granulosa cell proliferation and their estrogen secretion.
There are few studies investigating the RNA-binding protein Lin28B and ovine puberty. This study...