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Articles | Volume 66, issue 3
Articles | Volume 66, issue 3
https://doi.org/10.5194/aab-66-217-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/aab-66-217-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 66, issue 3
Original study
 | 
28 Jul 2023
Original study |  | 28 Jul 2023

Lin28B overexpression decreases let-7b and let-7g levels and increases proliferation and estrogen secretion in Dolang sheep ovarian granulosa cells

Zhiyuan Sui, Yongjie Zhang, Zhishuai Zhang, Chenguang Wang, Xiaojun Li, and Feng Xing

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Short summary
There are few studies investigating the RNA-binding protein Lin28B and ovine puberty. This study focuses on understanding how its overexpression changes microRNA levels, cell proliferation, and estrogen secretion. To assess these changes, we used quantitative real-time (qRT) PCR, overexpression vectors, and ELISA. We found that Lin28B overexpression decreases let-7b and let-7g levels and increases granulosa cell proliferation and their estrogen secretion.
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