Intrinsic challenges of neonatal adaptation in swine
The losses of piglets in commercial pig farming remain at concerning levels and need to be addressed through the implementation of new sustainable breeding and management strategies. In fact, piglets are especially at risk in the first days of life. Both genetics and the farrowing process have been shown to impact piglet vitality. In addition, knowledge of the animal-intrinsic responses in adapting to extra-uterine life is particularly important but is scarcely described in the scientific literature. In this review, the three phases that constitute neonatal adaptation in the pig are systematically presented. The first phase of early adaptation involves primarily the development of cardiorespiratory function (within the first 10 min of life) as well as thermoregulatory processes and acid–base balance (up to 24 h of life). In the second phase, homeostasis is established, and organ maturation takes place (up to 14 d post natum). The final third phase aims at the development of neurological, immunological and muscular features (up to 28 d of life). The involvement of aggravating and ameliorating factors such as dystocia, low colostrum yield and heat supply is key to the development of strategies to reduce piglet losses and increase vitality. The insights are of particular value in addressing current concerns in pig farming and to further improve animal welfare in pig production across different management types.
High piglet losses up to weaning are an ethical as well as economic challenge in various housing and management systems. In fact, the mortality currently remains around 15 %–25 %, which is equivalent to three to five piglets per litter in absolute terms given current litter sizes (Schild et al., 2020; Rootwelt, 2013). Furthermore, stillborn piglets account for another 6 % loss of total piglets born (Gourley et al., 2020). Consequently, the industry is obliged to further improve animal welfare aspects due to increased public perception.
It was observed that most losses happen within 3 d after parturition (Svendsen et al., 1991). A significant number of total losses already happen during the first 24 h after birth (Tuchscherer et al., 2000; Pandolfi et al., 2017; Devillers et al., 2011), and low-birth-weight piglets are overrepresented (Le Dividich et al., 2017). Most of these losses can be attributed to the “crushing” complex, which is associated with postnatal hypothermia and hypoglycemia (Tuchscherer et al., 2000). Modern hybrid lines in particular often produce litters with a high birth weight variation (i.e., a greater dispersion of birth weights), thus also producing light piglets with an increased risk of being crushed (Schild et al., 2020). In addition, colostrum production and litter size are not correlated in pigs, reducing nutrient intake per piglet and increasing the risk of crushing of individual piglets (Devillers et al., 2007). In this context, there are risks due to teat competition and the associated variation in the amount of colostrum available per piglet and therefore nutrient supply, which further increases the risk of crushing individual piglets (Mersmann, 1974; Devillers et al., 2007). Furthermore, in an investigation of 69 litters of hyper-prolific db.Viktoria sows, dystocia positively correlated with the risk of crushing her offspring (Blim, 2020; Lehn, 2020).
Taking into account the negative correlation of birth weight with pre-weaning death (Tuchscherer et al., 2000), it is necessary to elucidate the deficits of normally developed but lightweight piglets. Although neonatal pigs are born precocial, modern sow lines are thought to deliver a higher number of immature piglets (Mersmann, 1974; Fainberg et al., 2012; Schild et al., 2020). This immaturity might be one of the possible reasons for the lower viability and low birth weight of piglets, which is partly attributed to poorer placental supply (Mersmann, 1974; Fainberg et al., 2012; König et al., 2021). Management measures have been proposed to counteract the negative effects and associated increased piglet mortality, including genetic selection of breeding sows with regard to maternity behaviour, sow feeding, housing conditions at farrowing, hygiene and early individual care for at-risk neonates (Quesnel et al., 2008; Rutherford et al., 2013; Baxter et al., 2013).
The end of gestation and onset of birth are mediated by fetal cortisol. Although multiple authors conclude cortisol is not the single mediator of parturition in swine, the exact pathways inducing birth are not known yet (Silver, 1990; Nagel et al., 2019). Studies into this showed that lack of cortisol in hypophysectomized fetuses leads to a delayed parturition, but the injection of dexamethasone does not lead to the induction of birth as it does in ruminants (Randall, 1987). The adrenal function and uncoupling of hypothalamic–pituitary–adrenal feedback lead to high cortisol concentrations in late fetal development and ensure maturation of the lung as well as, in most species, leading to luteolysis and subsequently birth (Brooks et al., 1996).
Conflicting data exist on large litters and gestational length. It is conceivable that parturition occurs earlier because more fetuses contribute to cortisol secretion in larger litters, and a supposed threshold of cortisol concentration is reached sooner (Sasaki and Koketsu, 2007). On the other hand, Pietruszka et al. (2020) showed no difference of gestational length in large litters. An investigation into the corresponding gestational length is necessary, as there is an evident lack of knowledge about the stability of birth in modern highly prolific sows and the influence of shortened gestation due to hormonal administration at birth induction. It remains unclear as to whether high-prolific sows exhibit “birth stability”, i.e., whether physiological farrowing can occur consistently without physical intervention or administration of obstetric drugs.
The release of (stress) hormones during parturition induces a “kick start” in the piglet's metabolism, as is shown by a surge of thyroxine, catecholamines and cortisol. The mostly inactive, oxygen-deprived fetus with a low metabolism rate rapidly changes to a vigorous, active neonate with a high metabolism rate to ensure gain of temperature and locomotion to the sow's teats. The stress of birth is considered fundamental, as can be seen by (human) fetuses, which have decreased lung compliance when delivered by caesarean section (Gerten et al., 2005). Notably, sows show only passive maternal care (e.g., warmth, protection) during parturition and do not attend to their offspring to the same degree as uniparous mammals do (e.g., licking of the newborns, assistance in cutting the umbilical cord).
Multiple studies on pigs show a significant role of a prematurely ruptured umbilical cord in reduced viability. This is explained by a stronger acidosis and possibly plays a role in as much as 57 % of neonatal losses (Langendijk and Plush, 2019; Gourley et al., 2020; Rootwelt, 2013). A correlation between length of parturition and the condition of umbilical cord was shown (Gourley et al., 2020; Rootwelt, 2013). Nevertheless, some studies do not show an influence of the umbilical cord's status on weight gain, indicating that piglets are able to reconcile (Nuntapaitoon et al., 2018).
These survival-related factors, i.e., litter size, gestational length, birth induction, and umbilical cord status, form the basis of the pig's neonatal adaptation. As for all mammals, the latter is categorized into three phases, which are distinguished by specific functional, metabolic and endocrine characteristics. This review on porcine neonates focuses on these individual factors of the offspring. Based on the clinical classification of neonatal adaptation in multiparous (canine) species, the authors propose the usage of the following classification for neonatal piglet adaptation to characterize associated disorders (Bostedt, 2016).
The first phase of neonatal adaptation in piglets extends from birth to 24 h of life and is further subdivided. Establishment of cardiorespiratory function is the first challenge and is the characterizing event of the first subphase. It lasts about 10 min in swine. In the fetus, a bypass by the foramen ovale and ductus arteriosus exists, directing venous blood to the left atrium and aorta respectively, thereby circumventing the lung. After birth, those shunts close due to changing pressures. While the foramen ovale closes quickly after birth, the ductus arteriosus persists for a couple of days, enabling a left–right shunt (e.g., blood from the aorta flowing into the arteria pulmonalis). The neonate is solely dependent on heart rate to adapt cardiac output per minute with normal frequencies >200 beats per minute (bpm). This mechanism alone is not sufficient to regulate circulation in stressful situations. An unstable blood pressure and low stress tolerance result.
The same as cardiac function, respiratory function is, to a degree, impaired after birth, and high breathing frequencies result. Due to the soft rips, which complicate passive inspiration, the diaphragm is the motor of respiration. All of this leads to a physiological weakness of compensatory capacity. Not least, the start of respiratory function is possible only when alveolar ventilation is ensured by adequate surfactant and lung maturity.
Within the first few hours of life, the neonatal piglet needs to take up colostrum in order to gain passive immunity and meet energy demands. To enable location of the teats, concepts of “the udder” are inherited, and the piglet searches for a vertical plane (i.e., the lying sow). This is quite different to other (farm) animals where the mother stands, and a horizontal surface is imprinted (Walser and Bostedt, 2008). The neonates spent the remaining hours of the first phase with accommodation to the new environment and building a relationship with the sow and their littermates. The closure of the intestinal barrier for globulins within the first 24 h should be discussed as the endpoint of the first phase in pigs.
The following second phase spans over the first 2 weeks of the piglets' life. Now the parenchymal organs mature, with the intestines and pancreas experiencing the highest relative growth (Walser and Bostedt, 2008). The ribs harden, and heart rate declines. Amongst other mechanisms, the peripheral vasoconstriction matures, enabling improved circulatory and blood pressure regulation. Further, the ductus arteriosus closes, marking an important step in postnatal development of the heart. Hepatic function matures, and the liver ensures the synthesis of proteins. In addition, kidney function reaches physiological levels, and homeostasis of plasma molarity and volume can be maintained. As pigs are born with mature neurological and locomotor function, the second phase focuses on the metabolism and further strengthening of homeostasis.
The third phase lasts until 4 weeks after birth and is characterized by ethological development, further muscular growth and neurological improvement. As maternal immunoglobulin G (IgG) begins to decline in the first week of life, their concentration is surpassed by piglet-produced IgG around the fourth to fifth week of life. Consequently the intestinal immunoglobulin A (IgA) system develops, and their own IgG is built in the liver (Walser and Bostedt, 2008). While the maternal immunoglobulins decline, and the piglet's immunocompetence is still developing, a window of immunological weakness arises, which ranges from day 28 to about day 56 of life (Juul-Madsen et al., 2010).
For a successful neonatal adaptation, the series of events must proceed in order. Most complaints in neonatal pigs can be thematically assigned to acid–base balance, thermoregulation and energy allocation. Their systemic significance during the temporal course of the phase model is described below.
The fetus is able to develop despite low oxygen tension, once coined as “Everest in utero” (Eastmann, 1954). Comline and Silver (1974) described partial oxygen pressure (pO2) around 60 mm Hg in the maternal and 20–30 mm Hg in the fetal artery in pigs. This is a far wider pO2 gap than in horses, as a species with the most similarity in terms of placentation (Wooding and Burton, 2008). A gap also exists in pH, with fetal values in swine being 0.1 lower than maternal ones. To attenuate the effects of hypoxia in utero, three major mechanisms have developed. First, the oxygen binding capacity is elevated in the fetus. This is mainly reached by a reduction of 50 % in concentration of 2,3-bisphosphoglyceric acid (2,3-BPG) (Comline and Silver, 1974), as there is no hemoglobin-isoform. Despite the allometry of energy need, as defined in Kleiber's law, demanding a high energy need in fetuses, the actual demand is similar to their mother's (Dawes and Mott, 1959). Amongst others, this is possible because no thermoregulation is needed in utero. In addition, high-demand organs (e.g., brain, liver) of the fetus have a lower energy need than in the newborn, decreasing the total energy demand in utero. It is supposed that the underlying mechanism in the brain is a reduced membrane leakiness, saving energy normally needed for upholding the membrane potential (Hansen, 1977; Xia and Haddad, 1994). The lower oxygen consumption also enables a deeper tissue oxygenation at low tensions (Krogh, 1919). While the main energy is required for growth and development, it seems plausible that the fetus can slow the growth to save energy. Thirdly and auxiliary to these general adjustments, a diving reflex like behavior in temporary shortened oxygen supply exists. Here hypoxic events are answered by bradycardia, slowing blood flow and centralization, limiting anaerobiosis to peripheral, non-essential tissue (Snyder, 1983). The accumulating lactate is, when the oxygen demand is met again, metabolized in the liver. The enhanced hypoxia resistance of neonatal hearts supports the transport due to stable heart function throughout the hypoxic events (Bove and Stammers, 1986). All this is to some degree still active in neonatal mammals and contributes to an upholding of hypoxia resistance within the first phase of early adaptation of life (Dawes and Mott, 1959). One should keep in mind the relative advanced neurological maturity in porcine neonates, compared to other precocial mammals, making them more sensitive to perinatal oxygen deprivation (Mellor and Lentle, 2015).
Because of ante- and intrapartum uterine contractions, neonates experience certain degrees of hypoxia, rendering the outlined mechanisms indispensable to marginalize the impact (Randall, 1971; Taverne et al., 1979). Until 15 min before the onset of birth, oxygen levels remain largely stable, and negative influences are reduced by centralization and slowing of blood circulation (i.e., diving reflex) as well as a reduction of movement (Randall, 1992). Uterine contractions along with compromised blood flow in the umbilical cord lower the available oxygen for the fetus, inducing an anaerobic milieu in the muscle and, if compensatory capacity is exceeded, subsequently in vital organs.
A certain amount of perinatal stress is normal, and catecholamine has vital functions in managing extra uterine life. When particularly strong perinatal distress is present, the rise in catecholamines has a fundamental impact on the metabolism, resulting in activation of glycolysis and hyperglycemia. This is accompanied by a negative prognosis (Tuchscherer et al., 2000; Mota-Rojas et al., 2011), since a higher metabolic rate further increases oxygen debt and degree of acidosis. It will be discussed later how this stress response further affects neonatal adaptation.
With leaving the birth canal, the first breath of the neonate usually occurs within 15 s and is mediated by central and peripheral chemoreceptors detecting hypercapnia (Randall, 1992). In order to establish breathing, the lung has to be ventilated for the first time. To accomplish this, the fetus “practices” breathing in utero, and muscular function is gained prior to birth (Koos and Rajaee, 2014). Furthermore, adrenaline released during birth stops secretion in the lung and enables absorption of fluids. A delay in onset of respiration can originate from a lack of surfactant, especially in immature piglets. More commonly, it is the result of intrapartum asphyxia, which can be caused by a depressant effect of acidosis on respiration (Alonso-Spilsbury et al., 2005). In contrast, big piglets seem to have problems passing the birth canal, resulting in deeper acidosis, probably when uterine weakness is present (Trujillo-Ortega et al., 2011; Pandolfi et al., 2017). Yet it has not been discussed explicitly why and if the big piglets are able to recover better from low pH values than low-birth-weight piglets.
Although in early adaptation, the newborn pig has higher O2 affinity compared with the adult post natum, the amount of 2,3-BPG and subsequently oxygen affinity declines (Comline and Silver, 1974). Oxygen concentration quickly rises after onset of respiration (Herpin et al., 1996) and is around 75 %–80 % within a few seconds (Casellas et al., 2004; Panzardi et al., 2013). Nuntapaitoon et al. (2018) measured pO2 at around 5 min after birth, achieving similar values to those measured after 1 or 24 h, leading to the conclusion that only a few minutes is necessary to establish sufficient oxygen saturations in viable piglets (Rootwelt, 2013; Panzardi et al., 2013). Thus, establishment of oxygen saturation marks the end of the first phase of early adaptation. However, oxygen levels measured post natum have a low prognostic value for neonatal viability. In contrast, concentrations of carbon dioxide or lactate are of high prognostic value (Gourley et al., 2020; Trujillo-Ortega et al., 2011; Singer, 1999). This can be explained by the well-established negative influence of metabolic–respiratory acidosis on piglet viability (Herpin et al., 1996; Tuchscherer et al., 2000). Hence, a disturbed acid–base balance can be discussed as one of the main contributors to perinatal death.
Nevertheless, neonatal acidosis is to a degree physiological, and neonates are able to endure the low pH values after parturition without detrimental effects on further life (Randall, 1971). In the adult mammal, pH is kept in between 7.21–7.59, and small deviations have detrimental effects. A pH value of 6.9–7.0 seems to be a cutoff in the neonate below which consecutive negative effects become apparent (Randall, 1971; Zaleski and Hacker, 1993).
With the start of normal respiratory function, the peripheral tissue is oxygenated, and lactate, which was produced in the muscle during birth, is set free in the first 15–30 min and leads to a post natum acid high (Walser and Bostedt, 2008). Subsequently pH correction is accomplished within 2 h of life (Wehrend et al., 2005), which is an important step in early adaptation. Three mechanisms, descending from fastest to slowest, are responsible for acid–base balance: buffer systems (bicarbonate, phosphate and proteins) in the blood, the respiratory rate for CO2 regulation and the renal change of carbonic anhydrase activity to regulate the secretion of bicarbonate.
Animal-intrinsic efforts to maintain homeostasis, within the first phase of neonatal adaptation, are further strengthened. They are dependent on renal function and tissue integrity. Kidney development in pigs is completed in the third phase of neonatal adaptation around the 21st day (Frazier, 2017). In mammalians with delayed acquisition of adult glomerular filtration rate (GFR), a thinner juvenile cortex with “fetal” glomeruli morphology can persist until around 8 weeks post natum in the pig (Bergelin and Karlsson, 1975; Friis, 1980).
Neonatal anemia is common in piglets, and the occurrence increases in big litters (Steinhardt et al., 1984). This is an important factor, since hemoglobin serves as important buffer and is essential for oxygen transport (Bünger et al., 1988). The anemic piglet experiences all phases of a manifest iron deficiency in the first 72 h of life (Lemacher and Bostedt, 1995, 1994). This has an impairing effect on early adaptation, with consequences for erythropoiesis and iron-dependent enzymes. They showed a recourse on liver stored iron at the end of the first phase of adaptation. The usual supplementation of iron takes place later in a piglet's life and does not extenuate the effects completely, which can develop to a significant handicap (Lemacher and Bostedt, 1995, 1994). It should also be mentioned that neonatal anemia worsens thermotolerance, most likely due to lack of oxygenation (Mayfield et al., 1987).
The loss of ambient temperature after expulsion decreases body temperature of neonates by up to 3.5 ∘C (Vande Pol et al., 2020) and is, after establishment of (i) respiration and (ii) homeostasis, the third problem in early adaptation (Herpin et al., 1996, 2002). Farm animal species differ in several aspects considering environment (e.g., seasonal differences or outdoor housing) and individual disposition (e.g., lack of fur). The drop in body temperature is increased by evaporation, attributable to the amniotic fluid (Vande Pol et al., 2020).
In the porcine species the lack of hair, the body proportions with a relatively large body surface area and only scarce adipose tissue result in a temperature around 34 ∘C needed for thermoneutrality (Malmkvist et al., 2006; Mount, 1959). This temperature is partly lowered by the effect of social huddling to 25–30 ∘C (Mount, 1968).
With temperature in farms being around 21 ∘C, the neonates have to activate compensatory mechanisms. One of those, present in adult mammals, is reduction of peripheral blood flow by vasoconstriction, which probably is deficient in neonatal piglets (Lossec et al., 1999), although in older studies a calculatory influence was shown (Mount, 1963). In terms of insulation, piglets can multiply their scarce subcutaneous body fat at birth by tripling it within 48 h (Elliot and Lodge, 1977). This is possible because the high alimentary fat intake is primarily used to increase body fat (Theil et al., 2014). Combined with a maturation of the adipocytes from multi- to unilocular, as described in the review by Loveau et al. (Louveau et al., 2016), the heat loss is reduced. Still the improved thermal homeostasis is mainly influenced by increased heat production (Mount, 1959, 1963).
Berthon et al. (1994) and Herpin et al. (2002) concluded an overall absence of non-shivering thermogenesis (NST) in pigs. Studies show that Suidae ancestors lost the ability to express uncoupling protein (UCP)-1 20 million years ago, and it is accepted that therefore they lack brown adipose tissue (BAT) (Fyda et al., 2020). Thus, pigs were thought to exclusively rely on shivering thermogenesis, with authors ascribing up to 97 % of heat production on shivering (Herpin et al., 2002). Herpin et al. (2002) suggested an increase of efficiency mediated by increased myofibril mass, blood supply and triad proliferation to compensate the disadvantage.
Most species of Suidae and other classes lacking BAT occupy tropical climate zones (Gaudry et al., 2017; Fyda et al., 2020). In contrast, wild boars (Sus scrofa) live in cold to moderate climate and have had to develop additional mechanisms to mitigate heat loss in offspring. This is accomplished by huddling and nest building (Berg et al., 2006). Although this behavior is also expressed in domesticated pigs, especially nest building is considerably compromised in modern pig production due to lack of nest building material and restricted space. The alternatively offered heat lamps in conventional stables bear low attraction to the piglets, and they tend to stay with each other or in the sow's proximity for the first couple of days (Hrupka et al., 2000). In particular, piglets with lower viability express this behavior longer (Weary et al., 1996). Low temperatures are thought to affect the survival of low-viability piglets (Pandolfi et al., 2017; Pedersen et al., 2011). In this context, a recent study on mechanisms of thermoregulation is noteworthy, as it suggests a muscle-derived form of non-shivering thermogenesis (NST) exists in wild boar (Nowack et al., 2019). The NST is mediated by the sarco-/endoplasmic reticulum Ca2+-ATPase (SERCA) and regulated by sarcolipin. The underlying mechanism and its contribution to heat tolerance in BAT-ablated mice have been described previously (Bal et al., 2016, 2012). Briefly, sarcolipin is able to mediate the uncoupling of SERCA in order to increase heat production and ATP hydrolysis. It seems plausible that this mechanism persists in domestic pig, which could explain the aforementioned increase of thermoregulatory efficiency. Further, mainly lipids seem to be used for muscular heat production, creating an interesting perspective on intramuscular fat (Herpin et al., 2004).
The observed resistance to cold temperatures in outdoor farms, where straw is readily provided and hut temperatures can get near freezing, is explained by the aforementioned behavioral strategies (Schild et al., 2019). Multiple studies (Kilbride et al., 2012; Baxter et al., 2012; Rangstrup-Christensen et al., 2018b; Leeb et al., 2019) have shown no increase of early piglet mortality in outdoor housing conditions compared to indoor housing. Schild et al. (2019) emphasize the importance of hut insulation in outdoor housing, and Algers and Jensen (1990) showed no influence of temperatures as low as −17 ∘C . It is indicated that heat in summer does have an effect on stillborn rate due to maternal stress (Schild et al., 2020; Rangstrup-Christensen et al., 2018a). Analysis of seasonal effects has to be done carefully, since multiple effects (e.g., straw bedding, air-conditioning in indoor warms, huts, light) can have an impact, and it is difficult to differentiate those. Research into this is in demand due to growing interest in organic produced food and animal welfare.
A genetic contribution to the phenotypic variation in thermal capacity is likely, as differences between Asian and European breeds, but also between closely related European commercial lines, have been described. Schild et al. (2020) highlighted the influence of breed, which was also described between traditional Asian domestic pigs and commercial European lines (Herpin et al., 1993).
Despite being described separately, it is most important to realize the connection between acid–base balance and body temperature. Acidotic piglets are less viable, thus complicating postnatal adaptation and ultimately increasing the risk to die from a crushing complex (Randall, 1971; Zaleski and Hacker, 1993). As will be explained below, the piglet has few endogenous resources and is highly dependent on early colostrum uptake. Herpin et al. (1996) showed that lactate concentrations at birth correlate with thermal resistance and death within 21 d of life. Therefore, a hypothermia–hypoglycemia crushing relationship was established.
As described above, normothermia is usually developed at the end of early adaptation, and the piglet exerts maximal metabolic efforts in reaching and keeping this temperature (Xiong et al., 2018; Malmkvist et al., 2006; Herpin et al., 2002). Coldness slows growth as energy necessary for anabolism is diverted to thermoregulation (Berthon et al., 1996). A negative impact on survival was shown by Pedersen et al. (2011), who observed a relation between low body temperature 2 h after birth and a risk of crushing. Thyroxine plays an important role as a postnatal surge of active thyroid hormones is followed by an increase of heat production (Ślebodziński, 1965, 1979; Mount, 1959). The utilization rate of thyroxine differs between species and is exceptionally high in neonatal pigs (Mount, 1959). Mostyn et al. (2006) found differences in thyroxine levels between pig breeds. While thyroid hormones mediate the calorigenic effect, Kaciuba-Uścilko (1971) and LeBlanc and Mount (1968) suggest a complementary role for adrenalin. To understand the initial situation porcine newborns face, it is important to understand the energy metabolism and available resources. Supposedly, these resources can enable homeostasis for up to 12 h (Mellor and Cockburn, 1986; Mccance and Widdowson, 1959).
The piglet's first external source of energy is colostrum. The amount of ingested colostrum correlates with heat production. This is hardly explainable by gastro-intestinal heat production alone, which accounts for about 10 % (Herpin et al., 2002). Trujillo-Ortega et al. (2011) have shown the dysregulation in thermoregulation following insufficient or absent colostrum intake. It is essential that colostrum be taken in soon after birth. Nevertheless, it should be kept in mind that in the first day of life, colostrum itself is not sufficient to sustain metabolism, and glycogen stores are necessary (Theil et al., 2014; Szymeczko et al., 2008). In addition, Le Dividich et al. (2017) and Devillers et al. (2011) showed that the minimal amount of 180 g colostrum needed by each piglet cannot necessarily be provided in hyper-prolific sows (Quesnel et al., 2012). The subsequent lack of energy and immunoglobulins is a particular problem for small and weak piglets.
Pathways of energy provision are insufficient at birth and hinder efficient use of the scarce resources, but most develop within hours of adaptation in the neonate (Mersmann, 1974). This metabolic immaturity is in contrast to the relative neurological maturity and can result in a theoretical imbalance in energy demand and supply if certain factors are off. It should be taken into consideration that maternal stress in late fetal development (e.g., third trimester) seems to affect the fetus' HPA axis (Schwerin et al., 2005). The implications for piglet viability are yet unknown.
One of those deficient pathways is found in the carbohydrate metabolism. As in most fetuses, fructose is the preferred substrate of the porcine fetus, the deficiency of fructokinase after birth marks the change of substrate for carbohydrate metabolism. The ability to metabolize fructose via fructokinase is not regained until day 9 (Bird and Hartmann, 1996). Thus, fructose is thought to be a marker for immaturity in neonates as it is quickly eliminated after birth and higher in less viable piglets. In the neonate, glucose is the main substrate pre-colostrum, especially if stressed by cold (Mellor and Cockburn, 1986). Steele et al. (1971) have shown a dysregulated glucose homeostatic response of newborn swine. A colostrum-induced maturation of glucoregulatory mechanism is likely and explains the adverse glucagon response in fasted individuals (Lepine et al., 1989).
Glycogen stores, created in late fetal development, are readily available at birth (Elliot and Lodge, 1977). Being mainly stored in liver and muscle, it is used subsequent to birth, reaching minimum values 12–24 and 24–36 h after birth respectively. Gluconeogenetic capacity is probably attenuated in early adaptation, as is shown in an in vitro study by Mersmann and Phinney (1973). Mellor and Cockburn (1986) estimated liver glycogen being sufficient to sustain homeothermia for 2 h, while in fact the fasted piglet develops hypothermia after 12 h. The muscle glycogen used for shivering plausibly explains the postponed developing hypothermia. In this context, it is once again important to point out the limited possibilities of hypoxic piglets, which cannot use those supplies.
Studies observing the respiratory quotient (RQ) suggest a change in substrate to lipids at around 6–12 h post natum, indicated by a decrease of the RQ (Mount, 1969). In the pig, lipid metabolism is hindered by an immature carnitine palmitoyltransferase 1 (CPT-1) and low pancreas lipase activity (Fainberg et al., 2012). CPT-1 is necessary to transport the long chain fatty acids, found in colostrum, into the mitochondria, hence beta-oxidation being less efficient. In contrast to other species, piglets do not develop a neonatal hyperketonemia as ketogenesis is slowly developed (Adams and Odle, 1993). Nevertheless, swine have to deal with the low-carbohydrate and high-fat content of colostrum. It is hypothesized that acetate production via CoA release is a consequence of an oversaturated citrate cycle and necessary to sustain thiolase function of beta-oxidation (Adams et al., 1997; Adams and Odle, 1993). This is further supported by 10- to 100-fold higher plasma concentrations (311 ± 18 µmol L−1 in newborns) of acetate than beta-hydroxybutyrate (6 ± 1 µmol L−1 in newborns) as described by Adams and Odle (1998). This values increase after suckling. Lin et al. (2010) showed in an in vitro study, that acetogenesis takes place in the mitochondria and therefore is proportional to CPT-1 activity. An increase with piglet age is observed and is one of the steps in maturation of the lipid metabolism. The reason behind this is a change in sensitivity of CPT-1 to the inhibitory effect of malonyl-CoA (Lepine et al., 1989). Furthermore, a positive relation of acetogenic activity with non-esterified fatty acid (NEFA) availability is observed, when fasted and fed individuals are compared. It can be concluded that acetate is the primary product of mitochondrial oxidation in pig; consequently the preferred substrate in porcine liver, brain and intestine and early regulation is brought about by kinetic CPT-1 inhibition (Steele et al., 1984; Darcy-Vrillon et al., 1996).
Boyd et al. (1982) showed different patterns for NEFA oxidation. Oleic acid (18:1) was the most pronounced and stearic acid (18:0) the least pronounced fatty acid. From the results of Theil et al. (2014) and Herpin et al. (2002), it can be concluded that the liver can oxidize long- and medium-chain fatty acids (LCFAs and MCFAs), while only LCFAs are a suitable substrate for muscular lipid metabolism and thus can contribute to heat production. Interestingly, in Chinese Meishan pig, the newborn animals are able to metabolize stearic acid to omega-9 forms (e.g., 18:1, 20:1), enabling a faster oxidation of them or synthesis of triglycerides (TGs) (Fainberg et al., 2012). Fatty acid patterns change later to mirror adult Meishan, becoming comparable to commercial European lines' patterns.
Lepine et al. (1989) showed a 6-fold increase in NEFAs during ad libitum colostrum intake. This also indicates a relation of beta-oxidation and gluconeogenesis with glycerol-3-phosphate dehydrogenase (GPDH) and pyruvate carboxylase (PC). GPDH is necessary to activate acyl-CoA and nicotinamide adenine dinucleotide hydrogen (NADH), while PC provides ATP for the gluconeogenesis. Cholesterol steadily increases during the first 21 d (Kabalin et al., 2017). This is explained by a high fat diet and improved lipid utilization via upregulated bile acid synthesis (Lewis et al., 2000). In contrast, TGs are twice as high on day 1 compared to day 7 to 21, respectively. The decrease in TGs can be explained in adipocyte maturation and lipogenesis (Kabalin et al., 2017; Louveau et al., 2016). An additional part of 4 %–8 % of heat production is due to protein metabolism (Mellor and Cockburn, 1986). The piglet has a low protein oxidation capacity, and amino acids are mainly used for protein biosynthesis (De Vos et al., 2015). Nevertheless, protein catabolism will occur in cold-stressed or starving piglets if energy demand cannot be met (Mccance and Widdowson, 1959).
The hepatic capability of producing insulin-like growth factor-1 (IGF-1) is impaired in neonatal swine. The growth hormone receptors have a low binding capacity, and low levels of IGF-1 are observed (Louveau et al., 2000). Therefore, the main source of IGF-1 is the colostrum. As lactogenic IGF-1 is not absorbed, an influence on gut maturation and growth is discussed (Burrin et al., 1997; Louveau et al., 2000). A stimulatory response of IGF-1 secretion to pig growth hormone (GH) can be seen, and the weaning weight increases if additional IGF-1 is supplemented (Wester et al., 1998). This leads to the conclusion that the somatotropic axis, albeit functional, is not a relevant factor in (early) neonatal adaptation (Louveau et al., 2000).
Further remarks on the influence of common conventional farming and recent breeding seem necessary, since they interfere with parturition and aggravate the challenges.
Longer birth durations have been reported in modern breeds (Björkman et al., 2017; Van Dijk et al., 2005). Van Dijk et al. (2006, 2005) and Panzardi et al. (2013) have shown a cumulative effect of farrowing duration for later born piglets so that they were less viable, but only a little effect of the individual birth duration was observed. The cumulative effect is explained by repeated episodes of contraction, resulting in momentary asphyxia. The asphyxia leads to an oxygen deficit at birth, which increases the debt to be overcome in the first adaptation processes. An additional effect of contraction strength was seen in the higher mortality of piglets when uterotonics are used (Zaremba et al., 2019). Higher individual birth intervals are related to stillborn piglets, but it remains unclear as to whether they die sub partu or ante partum (Devillers et al., 2011).
Birth induction possibly interferes negatively with the biology of birth and gestation. It is clear that treatment with oxytocin can increase the risk of stillbirth and is increased in high dosages (Muro et al., 2020). A disrupted uterine blood flow due to increased myometrium contraction is often discussed as responsible. In other polytocous species, evidence also points to the negative influence of longer birth intervals (Groppetti et al., 2010). In one study (Taverne et al., 1979) an electromyogram during spontaneous birth was done and showed simultaneous contractions over multiple parts. This indicates whole-muscular uterine contractions, which could affect the piglets born later. Langendijk et al. (2018) showed that if dystocia can be detected as early as the fourth piglet, it points to sow-related problems (e.g., lack of oxytocin). On the other side, a positive effect of carbetocin in reducing piglet hypoxia is seen due to shorter birth intervals, as well as a positive effect due to shorter birth durations (Wähner and Hühn, 2001; Ward et al., 2019). However, the use of carbetocin might have a negative effect on colostrum yield (Boonraungrod et al., 2018; Vongsariyavanich et al., 2021).
Although it is often discussed, an effect on piglet viability of a shorter gestation length due to a prostaglandin F2α use has not been proven (Kirkden et al., 2013). With regard to neonates, artificially shortened gestation possibly impairs fetal adaptation prior to birth (e.g., in lung development and energy metabolism). To the best of our knowledge, an explicit evaluation of the possible effects on the phases of early adaptation is not published yet.
Although conventional farming offers heat lamps for providing adequate ambient temperature, they come with limitations as piglets tend to stay with the sow. Straw bedding keeps temperature as well as thermotolerance, therefore enabling more energy for maturation and growth. Furthermore, nest-building behavior is expressed by sows and, if not satisfied (e.g., lack of material to manipulate), can lead to stress. It has been shown that cortisol interferes with oxytocin binding, and stress-induced cortisol evaluation could lead to dystocia (Nagel et al., 2019; Edwards et al., 2019; Yun et al., 2013). In addition, Edwards et al. (2019) describe a positive impact of nest-building material even after parturition, while Yun et al. (2013) observed a more careful behavior of the sows.
Beside the high litter sizes in modern high-performance pig production, there seems to be evidence that conventional farrowing crates can have an additional negative influence on birth under the current conditions. Greater prolongation and less capability to compensate dystocia are observable in fixated sows (Blim, 2020; Lehn, 2020). This does not appear true for sows allowed to farrow freely, although the overall losses might be the same. The relationship of piglet and sow is impaired by conventional crates (Thodberg et al., 1999). In aspects of environment, the piglet vitality appeared better in group farrowing or free farrowing than in farrowing crates, while the observed crushing risk was lower in farrowing crates (Damm et al., 2005; Lohmeier et al., 2020).
The characterization of neonatal adaptation is important to determine causes for piglet loss and possible follow-up intervention. This should focus on the introduced phases of adaptation and respective processes. Although there is sufficient knowledge about the neonatal metabolism in general, studies are needed which take genetic differences in breeds into account. In addition, the social demand for new husbandry systems must be addressed. Particular emphasis on low-birth-weight piglets is needed, including the evaluation of pathophysiological findings. This could be important to elaborate strategies in breeding or management to increase piglet vitality and animal welfare.
HB conceptualized the study. HB and KW acquired funding and supervised the study. BD, MO and AV were responsible for conducting the study and collecting information and data. All authors reviewed and edited the manuscript and approved the published version of the article.
The contact author has declared that none of the authors has any competing interests.
Ethical approval was not required for this review article.
Publisher's note: Copernicus Publications remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This work has received funding from Tönnies Forschung. The FBN provided their own matched funding.
This paper was edited by Steffen Maak and reviewed by Laurianne Canario and one anonymous referee.
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