Articles | Volume 60, issue 2
Arch. Anim. Breed., 60, 105–118, 2017
Arch. Anim. Breed., 60, 105–118, 2017

Original study 15 May 2017

Original study | 15 May 2017

Protein profile and physicochemical characteristics of meat of lambs fed diets supplemented with rapeseed oil, fish oil, carnosic acid, and different chemical forms of selenium

Wiesław Przybylski1, Elżbieta Żelechowska1, Marian Czauderna2, Danuta Jaworska1, Katarzyna Kalicka1, and Krzysztof Wereszka2 Wiesław Przybylski et al.
  • 1Warsaw University of Life Sciences, Faculty of Human Nutrition and Consumer Sciences, 02-776 Warszawa, Poland
  • 2The Kielanowski Institute of Animal Physiology and Nutrition – Polish Academy of Sciences, 05-110 Jabłonna, Poland

Abstract. The objective of this study was to evaluate the physicochemical characteristics of the longissimus muscle of lambs fed a control diet containing 3 % rapeseed oil (RO) (group I); an experimental diet with 2 % RO and 1 % fish oil (FO) (group II); or experimental diets with coupled addition of 2 % RO, 1 % FO, and 0.1 % carnosic acid (CA) (group III) without/with 0.35 ppm Se as selenized yeast (SeY) (group IV) or selenate (SeVI as sodium selenate, Na2SeO4) (group V). The results showed that dietary FO or SeVI affected the ultimate pH and meat color in comparison to lambs fed the control diet. The differences noted between diets in the case of muscle tissue were as follows: myosin HC (heavy chain) and LC1 (light chain), 150 kDa, α-actinin, 60 kDa, and TnT (troponin T), 30 kDa protein. The addition of FO to lamb diets resulted in a lower content of 30 kDa proteins, which are indicators of proteolysis. Considering the protein profile of drip loss, results showed effects of dietary administration of FO, CA, and Se (as SeY and SeVI) on the abundance of the following sarcoplasmic proteins: AMPDA (AMP deaminase), PGM (phosphoglucomutase), PK/PGI (pyruvate kinase/phosphoglucose isomerase), CK/PGAK (creatine kinase/phosphoglycerate kinase), ALD (aldolase), LDH (lactate dehydrogenase), PGAM (phosphoglycerate mutase), and TPI (triosephosphate isomerase). The lower content of TPI (in group II with comparison to groups III–V) and PGAM (in group II with comparison to group I and III) could be due to a higher ultimate pH in the group with FO addition, whereas lower contents of CK/PGAK, ALD, and LDH were attributed to Se addition. In comparison to the control diet, all experimental diets without SeVI decreased the content of the sum of all assayed amino acids (  AAs), indispensable amino acids (  IAAs), and dispensable amino acids (  DAAs) in the muscle. The highest ratios of   IAAs to   DAAs and   IAAs to   AAs were found in the muscle of lambs fed the diet containing RO, FO, and CA. The FO-supplemented diet with CA and SeVI increased the abundance of methionine in the muscle in comparison to the control diet and the FO-supplemented diet without/with CA.

Short summary
The aim of this study was to evaluate the meat quality of the longissimus muscle of lambs fed a diet containing 3 % rapeseed oil (RO) or a diet with 2 % RO and 1 % fish oil (FO), or experimental diets with addition of 2 % RO, 1 % FO, 0.1 % carnosic acid with 0.35 ppm Se as selenized yeast (SeY) or selenate (SeVI). The results showed that FO and SeVI affected the meat quality traits, especially ultimate pH, color, and muscle proteome profile as well as amino acid composition of muscle.