Articles | Volume 68, issue 1
https://doi.org/10.5194/aab-68-67-2025
https://doi.org/10.5194/aab-68-67-2025
Original study
 | 
31 Jan 2025
Original study |  | 31 Jan 2025

Identification of highly expressed genes and efficient core promoters specific to buffalo skeletal muscles

Jieping Huang, Duo Guo, Ruirui Zhu, Haopeng Wang, Chunyan Yang, Deshun Shi, and Jianghua Shang

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Cited articles

Chen, H. H., Maeda, T., Mullett, S. J., and Stewart, A. F. R.: Transcription cofactor Vgl-2 is required for skeletal muscle differentiation, Genesis, 39, 273–279, https://doi.org/10.1002/gene.20055, 2004. 
Feng, X., Cao, X., Zhu, R., and Huang, J.: Selection and validation of reference genes for RT-qPCR in adipose and longissimus dorsi muscle tissues of buffalo, Anim. Biotechnol., 33, 526–535, https://doi.org/10.1080/10495398.2020.1811715, 2022. 
Fraimovitch, E. and Hagai, T.: Promoter evolution of mammalian gene duplicates, BMC Biol., 21, 80, https://doi.org/10.1186/s12915-023-01590-6, 2023. 
Gao, Y., Wu, H., Wang, Y., Liu, X., Chen, L., Li, Q., Cui, C., Liu, X., Zhang J., and Zhang, Y.: Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects, Genome Biol., 18, 13, https://doi.org/10.1186/s13059-016-1144-4, 2017. 
Gu, H., Zhou, Y., Yang, J., Li, J., Peng, Y., Zhang, X., Miao, Y., Jiang, W., Bu, G., Hou, L., Li, T., Zhang, L., Xia, X., Ma, Z., Xiong, Y., and Zuo, B.: Targeted overexpression of PPARγ in skeletal muscle by random insertion and CRISPR/Cas9 transgenic pig cloning enhances oxidative fiber formation and intramuscular fat deposition, FASEB J., 35, e21308, https://doi.org/10.1096/fj.202001812RR, 2021. 
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Short summary
Intramuscular fat (IMF) is significant for the flavor and taste of buffalo meat. However, fat in subcutaneous and visceral depots is waste. Breeders desire to increase IMF deposition without affecting that in other depots. In the present study, two efficient promoters with specific transcriptional activity in the skeletal muscles of buffaloes were identified which can be used to drive the expression of adipogenesis genes and enhance IMF deposition in the skeletal muscles in buffaloes.
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