Articles | Volume 67, issue 1
https://doi.org/10.5194/aab-67-37-2024
© Author(s) 2024. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/aab-67-37-2024
© Author(s) 2024. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Physical and chemical properties, hygienic quality and fatty acid profile in milk of lactating Lacaune dairy sheep
Zvonko Antunović
Department of Animal Production and Biotechnology, Faculty of Agrobiotechnical Sciences Osijek, University of J. J. Strossmayer in Osijek, V. Preloga 1, 31000 Osijek, Croatia
Boro Mioč
Department of Animal Science and Technology, Faculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Department of Animal Production and Biotechnology, Faculty of Agrobiotechnical Sciences Osijek, University of J. J. Strossmayer in Osijek, V. Preloga 1, 31000 Osijek, Croatia
Ivan Širić
Department of Animal Science and Technology, Faculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Valentino Držaić
Department of Animal Science and Technology, Faculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Nataša Mikulec
Department of Dairy Science, Faculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, 10000 Zagreb, Croatia
Adela Krivohlavek
Andrija Štampar Teaching Institute of Public Health, Mirogojska 16, 10000 Zagreb, Croatia
Josip Novoselec
Department of Animal Production and Biotechnology, Faculty of Agrobiotechnical Sciences Osijek, University of J. J. Strossmayer in Osijek, V. Preloga 1, 31000 Osijek, Croatia
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The research was performed on 30 adult Lika Pramenka sheep, aiming to determine the influence of physiological stage on blood metabolic profile. Changes in blood indicators were expressed in heavily pregnant and lactating sheep. This research suggests that studies should include physiological status and feed quality in the determination of the metabolic profile of sheep's blood in order to obtain the best possible information on the herd.
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The research objective was to determine the blood mineral profile of French Alpine goats during the first third of the lactation period. The research was carried out on 20 5-year-old goats. Analysis of blood minerals and correlations between them can be useful in the determination of the animals’ health statuses during the first third of the lactation period as such information indicates the need for feed supplementation or the revision of the blood test reference range for specific goat breeds.
Cited articles
Alba, D. F., Favaretto, J. A., Marcon, H., Saldanha, T. F., Leal, K. W., Campigoto, G., Souza, C. F., Baldissera, M. D., Bianchi, A. E., Vedovatto, M., and Da Silva, A. S.: Vegetable biocholine supplementation in pre- and postpartum Lacaune sheep: Effects on animal health, milk production and quality, Small Ruminant. Res., 190, 106–165, https://doi.org/10.1016/j.smallrumres.2020.106165, 2020.
Alizadeh, A., Ehsani, M. R., and Rofehgarinejad, L.: Fatty acid profile and cholesterol content of Ghezel sheep milk during lactation period, Acta Alim., 46, 457–463, https://doi.org/10.1556/066.2017.46.4.8, 2017.
Angeles-Hernandez, J. C., Vieyra A. R., Kebreab, E., Appuhamy, J. A. D. R. N., Dougherty, H. C., Castelan-Ortega, O., and Gonzalez Ronquillo, M.: Effect of forage to concentrate ratio and fat supplementation on milk composition in dairy sheep: A meta-analysis, Livest. Sci., 238, 104069, https://doi.org/10.1016/j.livsci.2020.104069, 2020.
Antunović, Z., Novoselec, J., Šperanda, M., Steiner, Z., Ćavar, S., Pavlović, N., Valek Lendić, K., Mioč, B., Paćinovski, N., and Klir, Ž.: Monitoring of blood metabolic profile and milk quality of ewes during lactation in organic farming, Mljekarstvo, 67, 243–252, https://doi.org/10.15567/mljekarstvo.2017.0401, 2017.
Antunović, Z., Mioč, B., Klir Šalavardić, Ž., Širić, I., Držaić, V., Đidara, M., and Novoselec, J.: The effect of lactation stage on the hematological and serum related biochemical parameters of the Travnik ewes, Poljoprivreda, 27, 56–62, https://doi.org/10.18047/poljo.27.2.7, 2021.
Antunović, Z., Klir Šalavardić, Ž., Zmaić, K., and Novoselec, J.: Introduction of Lacaune dairy sheep in Croatian sheep breeding, in: Proceedings of the 4th International Symposium for Agriculture and Food, ISAF 2022, 12–14 October 2022, Ohrid, Republic of North Macedonia, 3–4, 2022a.
Antunović, Z., Mioč, B., Klir Šalavardić, Ž., Držaić, V., Širić, I., and Novoselec, J.: Phenotype characteristics of Lacaune sheep in Republic of Croatia, in: Proceedings of the 57th Croatian & 17th International Symposium on Agriculture, 19–24 June 2022, Vodice, Croatia, 405–410, 2022b.
Antunović, Z., Mioč, B., Klir Šalavardić, Ž., Širić, I., Držaić, V., Đidara, M., Džaja, A., and Novoselec, J.: Influence of lactation stages on haematological and biochemical parameters in blood of Lacaune dairy sheep, Mljekarstvo, 72, 261–269, https://doi.org/10.15567/mljekarstvo.2022.0407, 2022c.
Antunović, Z., Mioč, B., Klir Šalavardić, Ž., Širić, I., Držaić, V., Šerić, V., Mandić, S., and Novoselec, J.: The Changes in the blood's acid-base balance of the Lacaune sheep during different lactation stages, Poljoprivreda, 28, 58–65, https://doi.org/10.18047/poljo.28.2.8, 2022d.
AOAC: Official methods of analysis of AOAC, International Association of Analytical Communities, 2006.
Barillet, F., Marie, C., Jacquin, M., Lagriffoul, G., and Astruc, J. M.: The French Lacaune dairy sheep breed: use in France and abroad in the last 40 years, Livest. Prod. Sci., 71, 17–29, https://doi.org/10.1016/S0301-6226(01)00237-8, 2001.
Bendelja, D., Antunac, N., Mikulec, N., Vnučec, I., Mašek, T., Mikulec, Ž., and Havranek, J.: Urea concentration in sheep's milk, Mljekarstvo, 59, 3–10, 2009.
Brito, M. A., González, F. D., Ribeiro, L. A., Campos, R., Lacerda, L., Barbosa, P. R., and Bergmann, G.: Blood and milk composition in dairy ewes from southern Brazil: variations during pregnancy and lactation, Ciênc. Rural, 36, 942–949, 2006.
Cabiddu, A., Decandia, M., Addis, M., Piredda, G., Pirisi, A., and Molle, G.: Managing Mediterranean pastures in order to enhance the level of beneficial fatty acids in sheep milk, Small Ruminant. Res., 59, 169–180, https://doi.org/10.1016/j.smallrumres.2005.05.005, 2005.
Carlsson, J., Bergstrom, J., and Pehrson, B.: Variations with breed, age, season, yield, stage of lactation and herd in the concentration of urea in bulk milk and individual cow's milk, Acta Vet. Scand., 36, 245–254, https://doi.org/10.1186/BF03547693, 1995.
Chilliard, Y., Glasser, F., Ferlay, A., Bernard, L., Rouel, J., and Doreau, M.: Diet, rumen biohydrogenation and nutritional quality of cow and goat milk fat, Eur. J. Lipid Sci. Tech., 109, 828–855, https://doi.org/10.1002/ejlt.200700080, 2007.
Conte, G., Palombo, V., Serra, A., Correddu, F., D'Andrea, M., Macciotta, N. P. P., and Mele, M.: Study of the fatty acid profile of milk in different sheep breeds: Evaluation by multivariate factorial analysis, Animals, 12, 722, https://doi.org/10.3390/ani12060722, 2022.
Correddu, F., Cellesi, M., Serdino, J., Manca, M. G., Contu, M., Dimauro, C., Ibba, I., and Macciotta, N. P. P.: Genetic parameters of milk fatty acid profile in sheep: Comparison between gas chromatographic measurements and Fourier-Transform Infrared Spectroscopy predictions, Animal, 13, 469–476, https://doi.org/10.1017/S1751731118001659, 2019.
De La Fuente, L. F., Barbosa, E., Carriedo, J. A., Gonzalo, C., Arenas, R., Fresno, J. M., and San Primitivo, F.: Factors influencing variation of fatty acid content in ovine milk, J. Dairy Sci., 92, 3791–3799, https://doi.org/10.3168/jds.2009-2151, 2009.
Fernandez, A. I. R. and Rodriguez, A. G.: Effect of dietary and animal factors on milk fatty acids composition of grazing dairy cows: a review, Iran. J. Appl. Anim. Sci., 2, 97–109, 2012.
Flis, Z. and Molik, E.: Importance of Bioactive Substances in Sheep's Milk in Human Health, Int. J. Mol. Sci., 22, 4364, https://doi.org/10.3390/ijms22094364, 2021.
Gatzias, I. S., Karabagias, I. K., Kontakos, S. P., Kontominasaand, M. G., and Badeka, A. V.: Characterization and differentiation of sheep'smilk from Greek breeds based onphysicochemical parameters, fatty acidcomposition and volatile profile, J. Sci. Food Agric., 98, 3935–3942, https://doi.org/10.1002/jsfa.8914, 2018.
González-García, E., Tesniere, A., Camous, S., Bocquie, F., Barillet, F., and Hassoun, P.: The effects of parity, litter size, physiological state, and milking frequency on the metabolic profile of Lacaune dairy ewes, Domest. Anim. Endocrin., 50, 32–44, https://doi.org/10.1016/j.domaniend.2014.07.001, 2015.
Gonzalo, C., Tardáguila, A., Ariznabarreta, A., Romeo, M., Montoro, V., Pérez-Guzmán, M. D., and Marco, J. C.: Somatic cell counts in dairy livestock and control strategies: Situation in Spain, in: Mastitis and milk quality, edited by: Contreras, A., Sánchez, A., and Romero, J. C., Diego-Marin Publ., Murcia, Spain, 145–151, 2000.
Hassoun, P., Cordoba, M. A., Parisot, S., Portes, D., Pradel, J., and Bocquier, F.: Effects of different supplement amounts on dry matter intake, milk production and milk composition of high-producing Lacaune dairy ewes, Lives. Sci., 251, 104664, https://doi.org/10.1016/j.livsci.2021.104664, 2021.
Inostroza, K., Bravo, S., Larama, G., Saenz, C., and Sepúlveda, N.: Variation in milk composition and fatty acid profile during the lactation of Araucana Creole ewes in a pasture-based system, Animals, 10, 92, https://doi.org/10.3390/ani10010092, 2020.
Jimenez, L. E. R., Hernandez, J. C. A., Palacios, C., Abecia, J. A., Naranjo, A., Avalos, J. O., and Gonzalez-Ronquillo, M.: Milk production of Lacaune sheep with different degrees of crossing with Manchega sheep in a commercial flock in Spain, Animals, 10, 520, https://doi.org/10.3390/ani10030520, 2020.
Kasapidou, E., Basdagianni, Z., Papadopoulos, V., Karaiskou, C., Kesidis, A., and Tsiotsias, A.: Effects of intensive and semi-intensive production on sheep milk chemical composition, physicochemical characteristics, fatty acid profile, and nutritional Indices, Animals, 11, 2578, https://doi.org/10.3390/ani11092578, 2021.
Kuchtik, J., Konečna, L., Sykora, V., Šustova, K., Fajman, M., and Kos, I.: Changes of physico-chemical characteristics, somatic cell count and curd quality during lactation and their relationship in Lacaune ewes, Mljekarstvo, 67, 138–145, https://doi.org/10.15567/mljekarstvo.2017.0206, 2017.
Kucuk O., Hess B. W., Ludden P. A., and Rule D. C.: Effect of forage/concentrate ratio on ruminal digestion and duodenal fl ow of fatty acids in ewes, J. Anim. Sci., 79, 2233–2240, https://doi.org/10.2527/2001.7982233x, 2001.
Legarra, A., Ramon, M., Ugarte, E., and Perez-Guzman, M. D.: Economic weights of fertility, prolificacy, milk yield and longevity in dairy sheep, Animal, 1, 193–203, https://doi.org/10.1017/S1751731107657814, 2007.
Li, R., Ma, Y., and Jiang, L.: Review: Research Progress of Dairy Sheep Milk Genes, Agriculture, 12, 169, https://doi.org/10.3390/agriculture12020169, 2022.
Libis-Márta, K., Póti, P., Egerszegi, I., Bodnár, A., and Pajor, F.: Effect of selected factors (body weight, age, parity, litter size and temperament) on the entrance order into the milking parlour of Lacaune ewes, and its relationship with milk production, J. Anim. Feed Sci., 30, 111–118, https://doi.org/10.22358/jafs/135727/2021, 2021.
Makovicky, P., Nagy, M., Makovicky, P., and Szineta, C.: Milk quality comparison of the sheep breeds (Improved Valachian, Tsigai, Lacaune) and their crosses, Magy. Allatorvosok, 135, 85–90, 2013.
Martini, M., Liponi, G. B., and Salari, F.: Effect of forage:concentrate ratio on the quality of ewe's milk, especially on milk fat globules characteristics and fatty acids composition, J. Dairy Res., 77, 239–244, https://doi.org/10.1017/S0022029910000038, 2010.
Mehaba, N., Coloma-Garcia, W., Such, X., Caja, G., and Salama, A. A. K.: Heat stress affects some physiological and productive variables and alters metabolism in dairy ewes, J. Dairy Sci., 104, 1099–1110, https://doi.org/10.3168/jds.2020-18943, 2021.
Mierlita, D., Daraban, S., and Lup, F.: Effects of breed on milk fatty acid profile in dairy ewes, with particular reference to cis-9, trans-11 conjugated linoleic acid, S. African Soc. Anim. Sci., 441, 223–231, 2011.
Molina, E., Ferret, A., Caja, G., Calsamiglia, S., Such, X., and Gasa, J.: Comparison of voluntary food intake, apparent digestibility, digesta kinetics and digestive tract content in Manchega and Lacaune dairy sheep in late pregnancy and early and mide lactation, Anim. Sci., 72, 209–221, https://doi.org/10.1017/S1357729800055703, 2001.
Nedeva, I., Slavov, T., Varlyakov, I., Radev, V., Panayotov, D., and Nedelkov, K.: Behavior of Lacaune sheep in a milking parlour, Bulg. J. Agric. Sci., 25, 74–80, 2019.
Novotna, L., Kuchtik, J., Sustova, K., Zapletal, D., and Filipcik, R.: Effects of lactation stage and parity on milk yield, composition and properties of organic sheep milk, J. Appl. Anim. Res., 36, 71–76, https://doi.org/10.1080/09712119.2009.9707034, 2009.
Nudda, A., Cannas, A., Correddu, F., Atzori, A. S., Lunesu, M. F., Battacone, G., and Pulina, G.: Sheep and goats respond differently to feeding strategies directed to improve the fatty acid profile of milk fat, Animals, 10, 1290, https://doi.org/10.3390/ani10081290, 2020.
Oravcová, M., Margetín, M., Peškovičová, D., Daňo, J., Milerski, M., Hetényi, L., and Polák, P.: Factors affecting milk yield and ewe's lactation curves estimated with test-day models, Czech J. Anim.Sci., 51, 483–490, https://doi.org/10.17221/3968-CJAS, 2006.
Panayotov, D., Sevov, S., and Georgiev, D.: Milk yield and morphological characteristics of the udder of sheep from the breed Lacaune in Bulgaria, Bulg. J. Agric. Sci., 24, 95–100, 2018a.
Panayotov, D., Naydenova, N., Mihaylova, G., and Iliev, T.: Physico-chemical and technological characteristics of Lacaune ewe's milk, Bulg. J. Agric. Sci., 24, 101–108, 2018b.
Panayotov, D., Naydenova, N., Iliev, T., and Mihaylova, G.: Agricultural Academy Fatty acids profile and lipids health indices of white brined cheese produced from Lacaune sheep milk, Bulg. J. Agric. Sci., 25, 85–90, 2019.
Park, Y. W., Juarez, M., Ramos, M., and Haenlein, G. F. W.: Physico-chemical characteristics of goat and sheep milk, Small Ruminant. Res., 68, 88–113, https://doi.org/10.1016/j.smallrumres.2006.09.013, 2007.
Pellegrini, O., Remeuf, F., Rivemale, M., and Barillet, F.: Renneting properties of milk from individual ewes: Influence of genetic and non-genetic variables, and relationship with physioco-chemical characteristics, J. Dairy Res., 64, 355–366, https://doi.org/10.1017/S0022029997002203, 1997.
Pulina, G., Nudda, A., Battacone, G., and Cannas, A.: Effects of nutrition on the contents of fat, protein, somatic cells, aromatic compounds, and undesirable substances in sheep milk, Anim. Feed Sci. Tech., 131, 255–291, https://doi.org/10.1016/j.anifeedsci.2006.05.023, 2006.
Sánchez, J. P., San Primitivo, F., Barbosa, E., Varona, L., and De la Fuente, L. F.: Genetic determination of fatty acid composition in Spanish Churra sheep milk, J. Dairy Sci., 93, 330–339. https://doi.org/10.3168/jds.2009-2268, 2010.
Sevi, A., Albenzio, M., Taibi, L., Dantone, D., Massa, S., and Annicchiarico, G.: Changes of somatic cell count through lactation and their effects on nutritional, renneting and bacteriological characteristics of ewe's milk, Adv. Food Sci., 21, 122–127, 1999.
Sinanoglou, V. J., Koutsouli, P., Fotakis, C., Sotiropoulou, G., Cavouras, D., and Bizelis, I.: Assessment of lactation stage and breed effect on sheep milk fatty acid profile and lipid quality indices, Dairy Sci. Technol., 95, 509–531, https://doi.org/10.1007/s13594-015-0234-5, 2015.
Soják, L., Blaško, J., Kubinec, R., Górová, R., Addová, G., Ostrovský, I., and Margetín, M.: Variation among individuals, breeds, parities and milk fatty acid profile and milk yield of ewes grazed on pasture, Small Ruminant. Res., 109, 173–181, https://doi.org/10.1016/j.smallrumres.2012.07.017, 2013.
Tatar, V., Tänavots, A., Polikarpus, A., Sats, A., Arvi, E., Mahla, T., and Jõudu, I.: Effect of the lactation months on milk composition of the second-parity Lacaune ewes, Agr. Res., 20, 424–436, https://doi.org/10.15159/AR.21.164, 2022.
Tsiplakou, E., Kominakis, A., and Zervas, G.: The interaction between breed and diet on CLA and fatty acids content of milk fat of four sheep breeds kept indoors or at grass, Small Ruminant. Res., 74, 179–187, https://doi.org/10.1016/j.smallrumres.2007.06.008, 2008.
Short summary
This paper explains the influence of a sheep's lactation stage on the physical and chemical properties, hygienic quality and fatty acids in milk produced by Lacaune sheep kept in intensive breeding. A significant increase in the content of fat, protein, total dry matter and casein and a significant decrease in the content of lactose in the late stage of lactation were observed. Analysis of fatty acids in milk proved a decrease in C18:2n6, C18:3n6, n6 and n6/n3 ratios during lactation.
This paper explains the influence of a sheep's lactation stage on the physical and chemical...