Articles | Volume 60, issue 2
https://doi.org/10.5194/aab-60-119-2017
https://doi.org/10.5194/aab-60-119-2017
Original study
 | 
17 May 2017
Original study |  | 17 May 2017

Variant GDF9 mRNA is likely not the main cause of larger litter size in Iranian Lori-Bakhtyari, Shal, Ghezel, and Afshari sheep breeds

Shahin Eghbalsaied, Farzad Rashidi Khorasgani, Hamid-Reza Amini, Majid Farahi, Maryam Davari, Ahmad Pirali, Sheila Pourali, Mahmood Vatankhah, Mahmud Rostami, and Hadi Atashi

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Cited articles

Abdoli, R., Zamani, P., Deljou, A., and Rezvan, H.: Association of BMPR-1B and GDF9 genes polymorphisms and secondary protein structure changes with reproduction traits in Mehraban ewes, Gene, 524, 296–303, 2013.
Abdoli, R., Zamani, P., Mirhoseini, S., Ghavi Hossein-Zadeh, N., and Nadri, S.: A review on prolificacy genes in sheep, Reprod. Domest. Anim., 51, 631–637, 2016.
Akbarpour, M., Houshmand, M., Ghorashi, A., and Hayatgheybi, H.: Screening for FecGH mutation of growth differentiation factor 9 gene in Iranian Ghezel sheep population, Cell j (Yakhteh), 2, 139–144, 2008.
Barzegari, A., Atashpaz, S., Ghabili, K., Nemati, Z., Rustaei, M., and Azarbaijani, R.: Polymorphisms in GDF9 and BMP15 associated with fertility and ovulation rate in Moghani and Ghezel sheep in Iran, Reprod. Domest. Anim., 45, 666–669, 2010.
Bassam, B. J., Caetano-Anollés, G., and Gresshoff, P. M.: Fast and sensitive silver staining of DNA in polyacrylamide gels, Anal. Biochem., 196, 80–83, 1991.
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Short summary
Sequencing of the GDF9 gene in 16 twin-birth, 4 triplet-birth, and 2 infertile ewes from Iranian sheep with verified segregation of G2–6 mutations, as well as a new G0 mutation, C25T, which causes L9F substitution in the signal peptide region. None of the triplet-birth or infertile ewes carried the G1, G4, G7, FecGE, G8, or FecGT mutations. In addition, using a large dataset comprised of 605 individuals showed a significant difference in the abundance of G1 and G4 mutations between breeds.