Articles | Volume 69, issue 2
https://doi.org/10.5194/aab-69-301-2026
© Author(s) 2026. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/aab-69-301-2026
© Author(s) 2026. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Influence of heat stress and nighttime recovery on ovarian follicle population, oocyte yield, and in vitro embryo production in Holstein heifers
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye
Ömer Faruk Yesilkaya
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye
Maide Gölbası
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Muğla Sıtkı Kocman University, Muğla, Türkiye
Ayse Sarı
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Necmettin Erbakan University, Konya, Türkiye
Sakine Ülküm Cizmeci
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye
Dursun Ali Dinc
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye
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In this study, short synchronization methods that do not contain estradiol before superstimulation in ovum pickup (OPU) applications were investigated. The gonadotropin-releasing hormone (GnRH), human chorionic gonadotropin (hCG), and dominant follicle removal (DFR) were used for follicular wave emergence. It was determined that DFR and hCG for follicular wave emergence increased oocyte yield and in vitro embryo production success in pre-superstimulation synchronization protocols.
Cited articles
Adhikari, M., Longman, R. J., Giambelluca, T. W., Lee, C. N., and He, Y.: Climate change impacts shifting landscape of the dairy industry in Hawai`i, Transl. Anim. Sci., 6, txac064, https://doi.org/10.1093/tas/txac064, 2022.
Alkan, H., Satilmis, F., Demirel, M. A., Bodu, M., Yesilkaya, O. F., Ciftci, M. F., and Alkan, K. K.: Does using microfluidic sperm sorting chips in bovine IVEP affect blastocyst development?, Reprod. Domest. Anim., 58, 1012–1020, https://doi.org/10.1111/rda.14398, 2023.
Alkan, K. K., Satilmis, F., Sonmez, G., Deniz, Y. E., Culha, M. H., Ciftci, M. F., and Alkan, H.: Putrescine supplementation improves the developmental competence of in vitro produced bovine embryos, Theriogenology, 231, 133–143, https://doi.org/10.1016/j.theriogenology.2024.10.017, 2025.
Alves, B. G., Alves, K. A., Martins, M. C., Braga, L. S., Silva, T. H., Alves, B. G., and Gambarini, M. L.: Metabolic profile of serum and follicular fluid from postpartum dairy cows during summer and winter, Reprod. Fertil. Dev., 26, 866–874, https://doi.org/10.1071/RD13102, 2014.
Armstrong, D. V.: Heat stress interaction with shade and cooling, J. Dairy Sci., 77, 2044–2050, https://doi.org/10.3168/jds.S0022-0302(94)77149-6, 1994.
Baruselli, P. S., Batista, E. O. S., Vieira, L. M., Ferreira, R. M., Guerreiro, B. G., Bayeux, B. M., and Gimenes, L. U.: Factors that interfere with oocyte quality for in vitro production of cattle embryos: effects of different developmental & reproductive stages, Anim. Reprod., 13, 264–272, https://doi.org/10.21451/1984-3143-AR861, 2018.
Baruselli, P. S., Abreu, L. Â. D., Paula, V. R. D., Carvalho, B., Gricio, E. A., Mori, F. K., and D'Occhio, M.: Applying assisted reproductive technology and reproductive management to reduce CO2-equivalent emission in dairy and beef cattle: a review, Anim. Reprod., 20, e20230060, https://doi.org/10.1590/1984-3143-AR2023-0060, 2023.
Berling, F., Castro, F. C. D., and Oliveira, A. C. D. S.: Infuence of heat stress on in vitro oocyte and embryo production in high-yielding Holstein cows, Ciênc. Anim. Bras., 23, e-71852E, https://doi.org/10.1590/1809-6891v23e-71852E, 2022.
Bertens, C. A., Stoffel, C., Crombie, M. B., Vahmani, P., and Penner, G. B.: The effects of dietary cation-anion difference and dietary buffer for lactating dairy cattle under mild heat stress with night cooling, J. Dairy Sci., 107, 10851–10868, https://doi.org/10.3168/jds.2024-25225, 2024.
Bó, G. A. and Mapletoft, R. J.: Evaluation and classification of bovine embryos, Anim. Reprod., 10, 344–348, 2013.
Brügemann, K., Gernand, E., König von Borstel, U., and König, S.: Defining and evaluating heat stress thresholds in different dairy cow production systems, Arch. Anim. Breed., 55, 13–24, https://doi.org/10.5194/aab-55-13-2012, 2012.
Cholakkal, I. K.: Seasonal variation in body temperature as indicator of thermal stress in tropical dairy cows, J. Trop. Agric., 63, 106–111, https://doi.org/10.63599/JTA.2025.1560, 2025.
Dash, S., Chakravarty, A. K., Singh, A., Upadhyay, A., Singh, M., and Yousuf, S.: Effect of heat stress on reproductive performances of dairy cattle and buffaloes: A review, Vet. World, 9, 235, https://doi.org/10.14202/vetworld.2016.235-244, 2016.
De Rensis, F., Saleri, R., Garcia-Ispierto, I., Scaramuzzi, R., and López-Gatius, F.: Effects of heat stress on follicular physiology in dairy cows, Animals, 11, 3406, https://doi.org/10.3390/ani11123406, 2021.
Ferré, L. B., Alvarez-Gallardo, H., Romo, S., Fresno, C., Stroud, T., Stroud, B., and Kjelland, M. E.: Transvaginal ultrasound-guided oocyte retrieval in cattle: State-of-the-art and its impact on the in vitro fertilization embryo production outcome, Reprod. Domest. Anim., 58, 363–378, https://doi.org/10.1111/rda.14303, 2023.
Gelaro, R., McCarty, W., Suárez, M. J., Todling, R., Molod, A., Takacs, L., and Zhao, B.: The modern-era retrospective analysis for research and applications, version 2 (MERRA-2), J. Climate, 30, 5419–5454, https://doi.org/10.1175/JCLI-D-16-0758.1, 2017.
Gendelman, M., Aroyo, A., Yavin, S., and Roth, Z.: Seasonal effects on gene expression, cleavage timing, and developmental competence of bovine preimplantation embryos, Reproduction, 140, 73–82, https://doi.org/10.1530/REP-10-0055, 2010.
Gómez-Guzmán, J. A., Parra-Bracamonte, G. M., and Velazquez, M. A.: Impact of heat stress on oocyte developmental competence and pre-implantation embryo viability in cattle, Animals, 14, 2280, https://doi.org/10.3390/ani14152280, 2024.
Halimi, A. H., Karaca, C., and Büyüktaş, D.: Evaluation of NASA POWER climatic data against ground-based observations in the Mediterranean and continental regions of Turkey, J. Tekirdag Agr. Facul., 20, 104–114, https://doi.org/10.33462/jotaf.1073903, 2023.
Hansen, P. J.: Reproductive physiology of the heat-stressed dairy cow: implications for fertility and assisted reproduction, Anim Reprod, 16, 497–507, https://doi.org/10.21451/1984-3143-AR2019-0053, 2019.
Hayden, C. B., Sala, R. V., Absalón-Medina, V. A., Motta, J. C., Pereira, D., Moreno, J. F., and García-Guerra, A.: Synchronization of follicle wave emergence before ovarian superstimulation with FSH and ovum pick-up improves in vitro embryo production in pregnant heifers, Theriogenology, 188, 71–78, https://doi.org/10.1016/j.theriogenology.2022.05.017, 2022.
Held-Hoelker, E., Haake, L. S., Kurzella, J., Schreiber, M., Dauben, C., Salilew-Wondim, D., and Hoelker, M.: Heat stress during maturation of bovine oocytes profoundly impacts the mitochondrial bioenergetic profile and causes endoplasmic reticulum stress in subsequent blastocysts, Biol. Reprod., 112, 1100–1113, https://doi.org/10.1093/biolre/ioaf070, 2025.
Hidaka, T., Fukumoto, Y., Yamamoto, S., Ogata, Y., and Horiuchi, T.: Variations in bovine embryo production between individual donors for OPU-IVF are closely related to glutathione concentrations in oocytes during in vitro maturation, Theriogenology, 113, 176–182, https://doi.org/10.1016/j.theriogenology.2018.03.002, 2018.
Kadokawa, H., Sakatani, M., and Hansen, P. J.: Perspectives on improvement of reproduction in cattle during heat stress in a future Japan, Anim. Sci. J., 83, 439–445, https://doi.org/10.1111/j.1740-0929.2012.01011.x, 2012.
Kanzawa, S., O'Meara, E., Funnell, B. J., Bangert, E. A., Garrett, E. F., Cardoso, F. C., and Wheeler, M. B.: The effects of heat stress on the population of the ovarian follicles and in vitro embryo production in dairy cows, Theriogenology, 253, 117790, https://doi.org/10.1016/j.theriogenology.2025.117790, 2025.
Karasahin, T., Alkan, H., Satilmis, F., Dursun, S., and Erdem, H.: Effect of flunixin meglumine treatment during and after embryo transfer on the pregnancy rate in cattle, Reprod. Domest. Anim., 56, 1555–1561, https://doi.org/10.1111/rda.14019, 2021.
Khan, I., Mesalam, A., Heo, Y. S., Lee, S. H., Nabi, G., and Kong, I. K.: Heat stress as a barrier to successful reproduction and potential alleviation strategies in cattle, Animals, 13, 2359, https://doi.org/10.3390/ani13142359, 2023.
Lee, J., Kim, D., Son, J., Kim, D., Jeon, E., Jung, D., and Choi, I.: Effects of heat stress on conception in Holstein and Jersey cattle and oocyte maturation in vitro, J. Anim. Sci. Technol., 65, 324, https://doi.org/10.5187/jast.2022.e113, 2023.
López-Gatius, F.: Can thermoregulatory response to heat stress be improved in lactating dairy cows? Insights from counter-current heat transfer systems impacting reproduction, J. Reprod. Dev., 71, 68–70, https://doi.org/10.1262/jrd.2024-101, 2025.
Mader, T. L., Davis, M. S., and Brown-Brandl, T.: Environmental factors influencing heat stress in feedlot cattle, J. Anim. Sci., 84, 712–719, https://doi.org/10.2527/2006.843712x, 2006.
Miętkiewska, K., Kordowitzki, P., and Pareek, C. S.: Effects of heat stress on bovine oocytes and early embryonic development – an update, Cells, 11, https://doi.org/10.3390/cells11244073, 4073, 2022.
Mzedawee, H. R. H., Kowsar, R., Moradi-Hajidavaloo, R., Shiasi-Sardoabi, R., Sadeghi, K., Nasr-Esfahani, M. H., and Hajian, M.: Heat shock interferes with the amino acid metabolism of bovine cumulus-oocyte complexes in vitro: a multistep analysis, Amino Acids, 56, 2, https://doi.org/10.1007/s00726-023-03370-6, 2024.
Pavani, K., Carvalhais, I., Faheem, M., Chaveiro, A., Reis, F. V., and da Silva, F. M.: Reproductive performance of Holstein dairy cows grazing in dry-summer subtropical climatic conditions: effect of heat stress and heat shock on meiotic competence and in vitro fertilization, Asian-Australas, J. Anim. Sci., 28, 334, https://doi.org/10.5713/ajas.14.0480, 2015.
Payton, R. R., Romar, R., Coy, P., Saxton, A. M., Lawrence, J. L., and Edwards, J. L.: Susceptibility of bovine germinal vesicle-stage oocytes from antral follicles to direct effects of heat stress in vitro, Biol. Reprod., 71, 1303–1308, https://doi.org/10.1095/biolreprod.104.029892, 2004.
Penev, T., Dimov, D., Vasilev, N., Mitev, J., Miteva, T., Marinov, I., and Stojnov, M.: Influence of heat stress on reproductive performance in dairy cows and opportunities to reduce its effects-a review, Agric. Sci. Technol., 13, https://doi.org/10.15547/ast.2021.01.001, 2021.
Petyim, S., Båge, R., Hallap, T., Bergqvist, A. S., Rodríguez-Martínez, H., and Larsson, B.: Two different schemes of twice-weekly ovum pick-up in dairy heifers: effect on oocyte recovery and ovarian function, Theriogenology, 60, 175–188, https://doi.org/10.1016/S0093-691X(02)01363-8, 2003.
Polsky, L. and Von Keyserlingk, M. A.: Invited review: Effects of heat stress on dairy cattle welfare, J. Dairy Sci., 100, 8645–8657, https://doi.org/10.3168/jds.2017-12651, 2017.
Rockett, P. L., Campos, I. L., Baes, C. F., Tulpan, D., Miglior, F., and Schenkel, F. S.: Phenotypic analysis of heat stress in Holsteins using test-day production records and NASA POWER meteorological data, J. Dairy Sci., 106, 1142–1158, https://doi.org/10.3168/jds.2022-22370, 2023.
Roth, Z.: Effect of heat stress on reproduction in dairy cows: insights into the cellular and molecular responses of the oocyte, Annu. Rev. Anim. Biosci., 5, 151–170, https://doi.org/10.1146/annurev-animal-022516-022849, 2017.
Roth, Z.: Cooling is the predominant strategy to alleviate the effects of heat stress on dairy cows, Reprod. Domest. Anim., 57, 16–22, https://doi.org/10.1111/rda.13765, 2020.
Sakatani, M.: Effects of heat stress on bovine preimplantation embryos produced in vitro, J. Reprod. Develop., 63, 347–352, https://doi.org/10.1262/jrd.2017-045, 2017.
Spiers, D. E., Spain, J. N., Ellersieck, M. R., and Lucy, M. C.: Strategic application of convective cooling to maximize the thermal gradient and reduce heat stress response in dairy cows, J. Dairy Sci., 101, 8269–8283, https://doi.org/10.3168/jds.2017-14283, 2018.
Tian, H., Liu, J., Chen, X., Li, S., Li, X., Mengal, K., and Wang, D.: Effects of ambient temperature and humidity on body temperature and activity of heifers, and a novel idea of heat stress monitoring, Anim. Prod. Sci., 61, 1584–1591, https://doi.org/10.1071/AN20156, 2021.
Torres-Júnior, J. D. S., De FA Pires, M., De Sa, W. F., Ferreira, A. D. M., Viana, J. H. M., Camargo, L. S. D. A., and Baruselli, P. S.: Effect of maternal heat-stress on follicular growth and oocyte competence in Bos indicus cattle, Theriogenology, 69, 155–166, https://doi.org/10.1016/j.theriogenology.2007.06.023, 2008.
Watanabe, Y. F., de Souza, A. H., Mingoti, R. D., Ferreira, R. M., Batista, E. O. S., Dayan, A., and Baruselli, P. S.: Number of oocytes retrieved per donor during OPU and its relationship with in vitro embryo production and field fertility following embryo transfer. Anim. Reprod., 14, 635–644, https://doi.org/10.21451/1984-3143-AR1008, 2018.
Wolfenson, D. and Roth, Z.: Impact of heat stress on cow reproduction and fertility, Anim. Front., 9, 32–38, https://doi.org/10.1093/af/vfy027, 2019.
Wrzecińska, M., Czerniawska-Piątkowska, E., and Kowalczyk, A.: The impact of stress and selected environmental factors on cows' reproduction, J. Appl. Anim. Res., 49, 318–323, https://doi.org/10.1080/09712119.2021.1960842, 2021.
Short summary
This study investigated the impact of continuous heat stress and nighttime recovery on the yield of oocytes and the production of embryos in Holstein heifers. Oocyte collection sessions in the same herd under identical care and feeding were categorized using the day’s peak and average heat–humidity load. It was observed that nighttime recovery had a positive effect on oocyte and blastocyst yield. This suggests that nighttime temperatures should be considered when evaluating heat stress.
This study investigated the impact of continuous heat stress and nighttime recovery on the yield...