Articles | Volume 69, issue 2
https://doi.org/10.5194/aab-69-301-2026
https://doi.org/10.5194/aab-69-301-2026
Original study
 | 
19 May 2026
Original study |  | 19 May 2026

Influence of heat stress and nighttime recovery on ovarian follicle population, oocyte yield, and in vitro embryo production in Holstein heifers

Muhammed Furkan Ciftci, Ömer Faruk Yesilkaya, Maide Gölbası, Ayse Sarı, Sakine Ülküm Cizmeci, and Dursun Ali Dinc

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Cited articles

Adhikari, M., Longman, R. J., Giambelluca, T. W., Lee, C. N., and He, Y.: Climate change impacts shifting landscape of the dairy industry in Hawai`i, Transl. Anim. Sci., 6, txac064, https://doi.org/10.1093/tas/txac064, 2022. 
Alkan, H., Satilmis, F., Demirel, M. A., Bodu, M., Yesilkaya, O. F., Ciftci, M. F., and Alkan, K. K.: Does using microfluidic sperm sorting chips in bovine IVEP affect blastocyst development?, Reprod. Domest. Anim., 58, 1012–1020, https://doi.org/10.1111/rda.14398, 2023. 
Alkan, K. K., Satilmis, F., Sonmez, G., Deniz, Y. E., Culha, M. H., Ciftci, M. F., and Alkan, H.: Putrescine supplementation improves the developmental competence of in vitro produced bovine embryos, Theriogenology, 231, 133–143, https://doi.org/10.1016/j.theriogenology.2024.10.017, 2025. 
Alves, B. G., Alves, K. A., Martins, M. C., Braga, L. S., Silva, T. H., Alves, B. G., and Gambarini, M. L.: Metabolic profile of serum and follicular fluid from postpartum dairy cows during summer and winter, Reprod. Fertil. Dev., 26, 866–874, https://doi.org/10.1071/RD13102, 2014. 
Armstrong, D. V.: Heat stress interaction with shade and cooling, J. Dairy Sci., 77, 2044–2050, https://doi.org/10.3168/jds.S0022-0302(94)77149-6, 1994. 
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Short summary
This study investigated the impact of continuous heat stress and nighttime recovery on the yield of oocytes and the production of embryos in Holstein heifers. Oocyte collection sessions in the same herd under identical care and feeding were categorized using the day’s peak and average heat–humidity load. It was observed that nighttime recovery had a positive effect on oocyte and blastocyst yield. This suggests that nighttime temperatures should be considered when evaluating heat stress.
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