This study aimed to understand the effects of adding low-dose oxytocin hormone into ram semen on the pregnancy rate and reproductive efficiency of sheep. A total of 53 out of 122 Chios ewes were inseminated with oxytocin hormone content, and successful pregnancy was achieved 90.56 % of them. In examination of the pregnancy rate, one of the indicators of reproductive performance, the difference between the groups is statistically significant (p = 0.046; p ≤ 0.05).