Articles | Volume 69, issue 1
https://doi.org/10.5194/aab-69-157-2026
© Author(s) 2026. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/aab-69-157-2026
© Author(s) 2026. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Combating bovine mastitis: current insights and future directions for a global dairy challenge
Shah Zeb Ahmad
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Wenjuan Zhao
State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China
Ye Feng
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Xubin Lu
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Fagang Zhong
State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China
Mengli Han
State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China
Rifat Ullah Jan
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Muhammad Irfan Khan
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Zhangping Yang
CORRESPONDING AUTHOR
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
Cited articles
Abdi, R. D., Gillespie, B. E., Ivey, S., Pighetti, G. M., Almeida, R. A., and Dego, O. K.: Antimicrobial resistance of major bacterial pathogens from dairy cows with high somatic cell count and clinical mastitis, Animals, 11, 131, https://doi.org/10.3390/ani11010131, 2021.
Abutarbush, S. M.: Veterinary medicine – a textbook of the diseases of cattle, horses, sheep, pigs and goats, Canadian Vet. J., Radostits OM, Gay CC, Hinchcliff KW, Constable PD. Saunders, USA, 2007, 51, ISBN 9780-7020-2777-2, 2010.
Al Emon, A., Hossain, H., Chowdhury, M. S. R., Rahman, M. A., Tanni, F. Y., Asha, M. N., Akter, H., Hossain, M. M., Islam, M. R., and Rahman, M. M.: Prevalence, antimicrobial susceptibility profiles and resistant gene identification of bovine subclinical mastitis pathogens in Bangladesh, Heliyon, 10, e34567, https://doi.org/10.1016/j.heliyon.2024.e34567, 2024.
Al-Harbi, H., Ranjbar, S., Moore, R. J., and Alawneh, J. I.: Bacteria isolated from milk of dairy cows with and without clinical mastitis in different regions of Australia and their AMR profiles, Frontiers in Veterinary Science, 8, 743725, https://doi.org/10.3389/fvets.2021.743725, 2021.
Ali, T., Kamran, n., Raziq, A., Wazir, I., Ullah, R., Shah, P., Ali, M. I., Han, B., and Liu, G.: Prevalence of mastitis pathogens and antimicrobial susceptibility of isolates from cattle and buffaloes in Northwest of Pakistan, Frontiers in Veterinary Science, 8, 746755, https://doi.org/10.3389/fvets.2021.746755, 2021.
Antanaitis, R., Juozaitienė, V., Jonike, V., Baumgartner, W., and Paulauskas, A.: Milk lactose as a biomarker of subclinical mastitis in dairy cows, Animals, 11, 1736, https://doi.org/10.3390/ani11061736, 2021.
Artem'eva, O., Pereselkova, D., Vinogradova, I., Kotkovskaya, E., Sivkin, N., and Zinovieva, N.: Screening of dairy cows' herd for presence in milk of hemolytic microorganisms in relation to somatic cell content, Agricultural Biology, 810–816, https://doi.org/10.15389/agrobiology.2015.6.810eng, 2015.
Aryeetey, R. N., Marquis, G. S., Timms, L., Lartey, A., and Brakohiapa, L.: Subclinical mastitis is common among Ghanaian women lactating 3 to 4 months postpartum, J. Hum. Lact., 24, 263–267, https://doi.org/10.1177/0890334408316077, 2008.
Awandkar, S. P., Kulkarni, M. B., and Khode, N. V.: Bacteria from bovine clinical mastitis showed multiple drug resistance, Vet. Res. Commun., 46, 147–158, https://doi.org/10.21203/rs.3.rs-356653/v1, 2022.
Benić, M., Maćešić, N., Cvetnić, L., Habrun, B., Cvetnić, Ž., Turk, R., Đuričić, D., Lojkić, M., Dobranić, V., and Valpotić, H.: Bovine mastitis: a persistent and evolving problem requiring novel approaches for its control-a review, Vet. Arhiv, 88, 535–557, https://doi.org/10.24099/vet.arhiv.0116, 2018.
Berry, E. and Hillerton, J.: The effect of an intramammary teat seal on new intramammary infections, J. Dairy Res., 85, 2512–2520, https://doi.org/10.3168/jds.S0022-0302(02)74334-8, 2002.
Berry, E. A., Hogeveen, H., and Hillerton, J. E.: Decision tree analysis to evaluate dry cow strategies under UK conditions, J. Dairy Res., 71, 409–418, https://doi.org/10.1017/S0022029904000433, 2004.
Bhattarai, A., Kaphle, K., and Adhikari, P.: A Review on “Bovine Sub-Clinical Mastitis in Nepal: Sustainable Management Strategy.”, International Journal of Food Science and Agriculture, 4, https://doi.org/10.26855/ijfsa.2020.03.012, 2020.
Bi, Y., Wang, Y. J., Qin, Y., Guix Vallverdú, R., Maldonado García, J., Sun, W., Li, S., and Cao, Z.: Prevalence of bovine mastitis pathogens in bulk tank milk in China, PloS One, 11, e0155621, https://doi.org/10.1371/journal.pone.0155621, 2016.
Boerhout, E., Vrieling, M., Benedictus, L., Daemen, I., Ravesloot, L., Rutten, V., Nuijten, P., Van Strijp, J., Koets, A., and Eisenberg, S.: Immunization routes in cattle impact the levels and neutralizing capacity of antibodies induced against S. aureus immune evasion proteins, Vet. Res., 46, 115, https://doi.org/10.1186/s13567-015-0243-7, 2015.
Boireau, C., Cazeau, G., Jarrige, N., Calavas, D., Madec, J.-Y., Leblond, A., Haenni, M., and Gay, É.: Antimicrobial resistance in bacteria isolated from mastitis in dairy cattle in France, 2006–2016, J. Dairy Res., 101, 9451–9462, https://doi.org/10.3168/jds.2018-14835, 2018.
Broucek, J., Mihina, S., Uhrincat, M., Lendelova, J., and Hanus, A.: Impact of gestation and lactation stage on the dairy cow response following removal to unfamiliar housing and milking system, Ital. J. Anim. Sci., 14, 3410, https://doi.org/10.4081/ijas.2015.3410, 2015.
Cagnacci, A.: Melatonin in relation to physiology in adult humans: Mini Review, J. Pineal Res., 21, 200–213, https://doi.org/10.1111/j.1600-079X.1996.tb00287.x, 1996.
Carpenter, J. S., Abelmann, A. C., Hatton, S. N., Robillard, R., Hermens, D. F., Bennett, M. R., Lagopoulos, J., and Hickie, I. B.: Pineal volume and evening melatonin in young people with affective disorders, Brain imaging and behavior, 11, 1741–1750, https://doi.org/10.1007/s11682-016-9650-2, 2017.
Carvalho-Sombra, T., Fernandes, D., Bezerra, B., and Nunes-Pinheiro, D.: Systemic inflammatory biomarkers and somatic cell count in dairy cows with subclinical mastitis, Veterinary and Animal Science, 11, 100165, https://doi.org/10.1016/j.vas.2021.100165, 2021.
Chen, X., Chen, Y., Zhang, W., Chen, S., Wen, X., Ran, X., Wang, H., Zhao, J., Qi, Y., and Xue, N.: Prevalence of subclinical mastitis among dairy cattle and associated risks factors in China during 2012–2021: A systematic review and meta-analysis, Res. Vet. Sci., 148, 65–73, https://doi.org/10.1016/j.rvsc.2022.04.007, 2022a.
Chen, Z., Wang, K., Guo, J., Zhou, J., Loor, J. J., Yang, Z., and Yang, Y.: Melatonin Maintains Homeostasis and Potentiates the Anti-inflammatory Response in Staphylococcus aureus-Induced Mastitis through microRNA-16b/YAP1, J. Agr. Food Chem., 70, 15255–15270, https://doi.org/10.1021/acs.jafc.2c05904, 2022b.
Chowdhury, M. S., Rahman, M. M., Hossain, H., Ghosh, P., Islam, M. R., Lamichhane, B., Gomaa, F. A., Selim, H., Hossain, M. M., and Helmy, Y.: Subclinical mastitis of buffaloes in Asia: Prevalence, pathogenesis, risk factors, antimicro-bial resistance, and current treatment strategies, Journal of Animal Science and Technology, e66, https://doi.org/10.5187/jast.2024.e66, 2024.
Cobirka, M., Tančin, V., and Slama, P.: Epidemiology and classification of mastitis, Animals, 10, 2212, https://doi.org/10.3390/ani10122212, 2020.
Crispie, F., Flynn, J., Ross, R. P., Hill, C., and Meaney, W. J.: Dry cow therapy with a non-antibiotic intramammary teat seal-a review, Irish Vet. J., 57, 412, https://doi.org/10.1186/2046-0481-57-7-412, 2004.
Cvetnić, L., Samardžija, M., Habrun, B., Kompes, G., and Benić, M.: Microbiological monitoring of mastitis pathogens in the control of udder health in dairy cows, https://www.slovetres.si/index.php/SVR/article/view/92 (last access: 12 December 2025), 2016.
Cvetnić, L., Samardžija, M., Duvnjak, S., Habrun, B., Cvetnić, M., Jaki Tkalec, V., Đuričić, D., and Benić, M.: Multi locus sequence typing and spa typing of Staphylococcus aureus isolated from the milk of cows with subclinical mastitis in Croatia, Microorganisms, 9, 725, https://doi.org/10.3390/microorganisms9040725, 2021.
Cvetnić, L., Špičić, S., Kompes, G., Habrun, B., Katalinić-Janković, V., Cvetnić, M., Zdelar-Tuk, M., Reil, I., Duvnjak, S., and Cvetnić, Ž.: Bovine mastitis caused by rapid-growth environmental mycobacteria, Veterinarska Stanica, https://doi.org/10.46419/vs.53.5.11, 2022.
Defante, L., Damasceno, J. C., Bánkuti, F. I., and Ramos, C. E. C. O.: Typology of dairy production systems that meet Brazilian standards for milk quality, Rev. Bras. Zootecn., 48, e20180023, https://doi.org/10.1590/rbz4820180023, 2019.
Deluyker, H., Gay, J., and Weaver, L.: Interrelationships of somatic cell count, mastitis, and milk yield in a low somatic cell count herd, J. Dairy Sci., 76, 3445–3452, https://doi.org/10.3168/jds.S0022-0302(93)77683-3, 1993.
Didanna, H. L. and Anja, A.: An overview of dairy production in selected African and Asian countries: challenges and opportunities for sustainability, J. Dairy Res., 1–8, https://doi.org/10.1017/S0022029925100885, 2025.
Doležal, O. and Kopunecz, P.: Management dojení, jeho optimalizace a hodnocení kvality dodávek mléka, Institut vzdělávání v zemědělství, https://search.worldcat.org/title/817049314 (last access: 18 February 2026), 2010.
dos Santos Nascimento, J., Fagundes, P. C., de Paiva Brito, M. A. V., Dos Santos, K. R. N., and de Freire Bastos, M. d. C.: Production of bacteriocins by coagulase-negative Staphylococci involved in bovine mastitis, Vet. Microbiol., 106, 61–71, https://doi.org/10.1016/j.vetmic.2004.10.014, 2005.
Dubuc, J., Duffield, T., Leslie, K., Walton, J., and LeBlanc, S.: Effects of postpartum uterine diseases on milk production and culling in dairy cows, J. Dairy Res., 94, 1339–1346, https://doi.org/10.3168/jds.2010-3758, 2011.
Eastridge, M.: Major advances in applied dairy cattle nutrition, J. Dairy Res., 89, 1311–1323, https://doi.org/10.3168/jds.S0022-0302(06)72199-3, 2006.
Esslemont, R. and Kossaibati, M.: Culling in 50 dairy herds in England, Veterinary Record, 140, 36–39, https://doi.org/10.1136/vr.140.2.36, 1997.
Ewida, R. M. and Al-Hosary, A. A.: Prevalence of enterotoxins and other virulence genes of Staphylococcus aureus caused subclinical mastitis in dairy cows, Veterinary World, 13, 1193, https://doi.org/10.14202/vetworld.2020.1193-1198, 2020.
FAO: OECD-FAO agricultural outlook 2023–2032, OECD Publishing, Paris, https://doi.org/10.1787/37c7b798-en, 2023.
Ferlazzo, N., Andolina, G., Cannata, A., Costanzo, M. G., Rizzo, V., Currò, M., Ientile, R., and Caccamo, D.: Is melatonin the cornucopia of the 21st century?, Antioxidants, 9, 1088, https://doi.org/10.3390/antiox9111088, 2020.
Ferreira, E. M., Romero, L. C., de Lourdes Ribeiro de Souza da Cunha, M., Malagó Junior, W., Camargo, C. H., Barioni Júnior, W., and Zafalon, L. F.: Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis, BMC Vet. Res., 18, 273, https://doi.org/10.1186/s12917-022-03364-8, 2022.
Fournel, S., Godbout, S., Ruel, P., Fortin, A., Duquette-Lozeau, K., Létourneau, V., Généreux, M., Lemieux, J., Potvin, D., and Côté, C.: Production of recycled manure solids for use as bedding in Canadian dairy farms: II. Composting methods, J. Dairy Res., 102, 1847–1865, https://doi.org/10.3168/jds.2018-14967, 2019.
Fredebeul-Krein, F., Schmenger, A., Wente, N., Zhang, Y., and Krömker, V.: Factors associated with the severity of clinical mastitis, Pathogens, 11, 1089, https://doi.org/10.3390/pathogens11101089, 2022.
Galano, A. and Reiter, R. J.: Melatonin and its metabolites vs oxidative stress: From individual actions to collective protection, J. Pineal Res., 65, e12514, https://doi.org/10.1111/jpi.12514, 2018.
Gantner, V., Jožef, I., Samardžija, M., Steiner, Z., Gantner, R., Solić, D., and Potočnik, K.: Varijabilnost u prevalenciji subkliničkog i kliničkog mastitisa i njegov utjecaj na mliječnost krava holštajnske i simentalske pasmine kao rezultat pariteta, Vet. Arhiv, 94, 269–284, https://doi.org/10.24099/vet.arhiv.2518, 2024.
Haley, D., de Passille, A. M., and Rushen, J.: Assessing cow comfort: Effects of two floor types and two tie stall designs on the behaviour of lactating dairy cows, Appl. Anim. Behav. Sci., 71, 105–117, https://doi.org/10.1016/S0168-1591(00)00175-1, 2001.
Hameed, K., Sender, G., and Korwin-Kossakowska, A.: Public health hazard due to mastitis in dairy cows, Anim. Sci. P., 25, 73–85, https://www.researchgate.net/publication/233388187_Public_health_hazard_due_to_mastitis_in_dairy_cows (last access: 12 December 2025), 2017.
Han, Z., Fan, Y., Yang, Z., Loor, J. J., and Yang, Y.: Mammary transcriptome profile during peak and late lactation reveals differentially expression genes related to inflammation and immunity in Chinese Holstein, Animals, 10, 510, https://doi.org/10.3390/ani10030510, 2020
Haq, I. U., Kamal, M., Swelum, A. A., Khan, S., Ríos-Escalante, P. R. D. l., and Usman, T.: Alarming multidrug resistance in Staphylococcus aureus isolated from raw milk of cows with subclinical mastitis: Antibiotic resistance patterns and occurrence of selected resistance genes, PloS One, 19, e0301200, https://doi.org/10.1371/journal.pone.0301200, 2024.
Hardeland, R. and Pandi-Perumal, S.: Melatonin, a potent agent in antioxidative defense: actions as a natural food constituent, gastrointestinal factor, drug and prodrug, Nutr. Metab., 2, 1–15, https://doi.org/10.1186/1743-7075-2-22, 2005.
Harjanti, D. W. and Sambodho, P.: Effects of mastitis on milk production and composition in dairy cows, IOP C. Ser. Earth Env., 518, 012032, https://doi.org/10.1088/1755-1315/518/1/012032, 2020.
Hassan, A., Salem, A., Elghandour, M., Hafsa, S. A., Reddy, P., Atia, S., and Vidu, L.: Humic substances isolated from clay soil may improve the ruminal fermentation, milk yield, and fatty acid profile: A novel approach in dairy cows, Anim. Feed Sci. Tech., 268, 114601, https://doi.org/10.1016/j.anifeedsci.2020.114601, 2020.
Haveri, M., Roslöf, A., Rantala, L., and Pyörälä, S.: Virulence genes of bovine Staphylococcus aureus from persistent and nonpersistent intramammary infections with different clinical characteristics, J. Appl. Microbiol., 103, 993–1000, https://doi.org/10.1111/j.1365-2672.2007.03356.x, 2007.
Hillerton, J. and Berry, E.: Treating mastitis in the cow – a tradition or an archaism, J. Appl. Microbiol., 98, 1250–1255, https://doi.org/10.1111/j.1365-2672.2005.02649.x, 2005.
Hisira, V., Zigo, F., Kadaši, M., Klein, R., Farkašová, Z., Vargová, M., and Mudroň, P.: Comparative analysis of methods for somatic cell counting in cow's milk and relationship between somatic cell count and occurrence of intramammary bacteria, Veterinary Sciences, 10, 468, https://doi.org/10.3390/vetsci10070468, 2023.
Hodges, R., Jones, Y., and Holland, J.: Characterisation of Staphylococci associated with clinical and subclinical bovine mastitis, New Zeal. Vet. J., 32, 141–145, https://doi.org/10.1080/00480169.1984.35099, 1984.
Hogeveen, H., Steeneveld, W., and Wolf, C. A.: Production diseases reduce the efficiency of dairy production: A review of the results, methods, and approaches regarding the economics of mastitis, Annu. Rev. Resour. Econ., 11, 289–312, https://doi.org/10.1146/annurev-resource-100518-093954, 2019.
Holko, I., Tančin, V., Vršková, M., and Tvarožková, K.: Prevalence and antimicrobial susceptibility of udder pathogens isolated from dairy cows in Slovakia, J. Dairy Res., 86, 436–439, https://doi.org/10.1017/S0022029919000694, 2019.
Hoque, M. N., Istiaq, A., Rahman, M. S., Islam, M. R., Anwar, A., Siddiki, A. Z., Sultana, M., Crandall, K. A., and Hossain, M. A.: Microbiome dynamics and genomic determinants of bovine mastitis, Genomics, 112, 5188–5203, https://doi.org/10.1016/j.ygeno.2020.09.039, 2020.
Hovinen, M. and Pyörälä, S.: Invited review: Udder health of dairy cows in automatic milking, J. Dairy Res., 94, 547–562, https://doi.org/10.3168/jds.2010-3556, 2011.
Hu, X., Li, S., Mu, R., Guo, J., Zhao, C., Cao, Y., Zhang, N., and Fu, Y.: The rumen microbiota contributes to the development of mastitis in dairy cows, Microbiology Spectrum, 10, 02512–02521, https://doi.org/10.1128/spectrum.02512-21, 2022.
Huma, Z. I., Sharma, N., Kour, S., Tandon, S., Guttula, P. K., Kour, S., Singh, A. K., Singh, R., and Gupta, M. K.: Putative biomarkers for early detection of mastitis in cattle, Anim. Prod. Sci., 60, 1721–1736, https://doi.org/10.1071/AN19539, 2020.
Islam, M. M., Hossain, M. I., Islam, M. S., Azam, M. G., and Sultana, S.: Prevalence, Antibiotic Resistance Patterns, and Virulence Factors of Staphylococcus aureus Isolates Associated with Bovine Mastitis in Northern Bangladesh, Heliyon, 11, e42107, https://doi.org/10.1016/j.heliyon.2025.e42107, 2025.
Jackson, P. G., Cockcroft, P. D., and Elmhurst, S.: Clinical examination of farm animals, Wiley Online Library, https://doi.org/10.1002/9780470752425, 2002.
Jadhav, P., Tarate, S., Bhuvana, M., Das, D., and Shome, B.: Somatic cell count as a monitoring system for hygienic milk production in India: A review, Asian Journal of Dairy & Food Research, 35, https://doi.org/10.18805/ajdfr.v35i4.6624, 2016.
Jeong, S. A., Song, J., Ham, J., An, G., Song, G., and Lim, W.: Tetraconazole interrupts mitochondrial function and intracellular calcium levels leading to apoptosis of bovine mammary epithelial cells, Pestic. Biochem. Phys., 191, 105366, https://doi.org/10.1016/j.pestbp.2023.105366, 2023.
Jones, G. and Bailey, T.: Understanding the basics of mastitis, Virginia Cooperative Extension, Publication No. 404–233, Virginia State University, https://vtechworks.lib.vt.edu/server/api/core/bitstreams/21447d06-36f4-448f-90df-18aebf51ea2a/content (last access: 12 December 2025), 2006.
Joy, F., Bharadwaj, B., and Mishra, J.: Assessing Milk Production and Quality during Mastitis Caused by a Variety of Pathogens in Dairy Cows, Revista Electronica de Veterinaria, 24, 96–105, https://doi/full/10.5555/20230460846, 2023.
Kaczorek-Łukowska, E., Małaczewska, J., Wójcik, R., Duk, K., Blank, A., and Siwicki, A.: Streptococci as the new dominant aetiological factors of mastitis in dairy cows in north-eastern Poland: analysis of the results obtained in 2013–2019, Irish Vet. J., 74, 2, https://doi.org/10.1186/s13620-020-00181-z, 2021.
Kakati, S. P., Saikia, G. K., Sharma, R. K., Bora, D. P., Borah, P., Gogoi, S. M., Deka, P., Konch, P., Konwar, N., and Arif, S. A.: Subclinical mastitis-its prevalence, risk analysis, and association with methicillin-resistant Staphylococcus aureus (mrsa) in certain districts of assam, india, Exploratory Animal & Medical Research, 14, 51–62, http//doi/10.52635/eamr/14(S2)51-62, 2024.
Karunathilaka, R., Arachchige, N., Gunawardana, G., and Deepal Chandana, G.: Inhibitory effect of neem (Azadirachta indica) plant extract on bovine and poultry pathogens from Sri Lanka, 2577, http://repository.ou.ac.lk/handle/94ousl/2577 (last access: 12 December 2025), 2024.
Kawecka-Grochocka, E., Zalewska, M., Kapusta, A., Ząbek, T., Rzewuska, M., Petrykowski, S., and Bagnicka, E.: Transcripts and protein levels of CSN1S1 and CSN3 genes in dairy cattle mammary gland secretory tissue during chronic staphylococcal infection, J. Dairy Res., 88, 73–77, https://doi.org/10.1017/S0022029921000145, 2021.
Keegan, A. D., Leonard, W. J., and Zhu, J.: Recent advances in understanding the role of IL-4 signaling, Faculty Reviews, 10, 71, https://doi.org/10.12703/r/10-71, 2021.
Kelly, A. L., Leitner, G., and Merin, U.: Milk quality and udder health: Test methods and standards, in: Encyclopedia of Dairy Sciences: Second Edition, Elsevier Inc., 894–901, https://doi.org/10.1016/B978-0-12-374407-4.00353-8, 2011.
Khan, A., Mushtaq, M. H., Din Ahmad, M. U., Chaudhry, M., and Khan, A. W.: Prevalence of clinical mastitis in bovines in different climatic conditions in kpk,(Pakistan), Science International, 27, 2289–2293, https://www.researchgate.net/publication/312121990 (last access: 5 March 2026), 2015.
Khan, M. Z., Khan, A., Xiao, J., Ma, Y., Ma, J., Gao, J., and Cao, Z.: Role of the JAK-STAT pathway in bovine mastitis and milk production, Animals, 10, 2107, https://doi.org/10.3390/ani10112107, 2020.
Kibebew, K.: Bovine mastitis: A review of causes and epidemiological point of view, Journal of Biology, Agriculture and Healthcare, 7, 1–14, https://www.iiste.org/Journals/index.php/JBAH/article/view/34975 (last access: 5 March 2026), 2017.
Kirkan, Ş., Göksoy, E. Ö., and Kaya, O.: Identification and antimicrobial susceptibility of Staphylococcus aureus and coagulase negative Staphylococci from bovine mastitis in the Aydın region of Turkey, Turk. J. Vet. Anim. Sci., 29, 791–796, 2005.
Klaas, I. and Zadoks, R.: An update on environmental mastitis: Challenging perceptions, Transb. Emerg. Dis., 65, 166–185, https://doi.org/10.1111/tbed.12704, 2018.
Kovačević, Z., Mihajlović, J., Mugoša, S., Horvat, O., Tomanić, D., Kladar, N., and Samardžija, M.: Pharmacoeconomic analysis of the different therapeutic approaches in control of bovine mastitis: Phytotherapy and antimicrobial treatment, Antibiotics, 12, 11, https://doi.org/10.3390/antibiotics12010011, 2022a.
Kovačević, Z., Samardžija, M., Horvat, O., Tomanić, D., Radinović, M., Bijelić, K., Vukomanović, A. G., and Kladar, N.: Is there a relationship between antimicrobial use and antibiotic resistance of the most common mastitis pathogens in dairy cows?, Antibiotics, 12, 3, https://doi.org/10.3390/antibiotics12010003, 2022b.
Kovacevic, Z., Samardzija, M., and Tomanic, D.: A review of recent developments in essential oil-based alternatives in mastitis treatment in dairy cows, Annals of Animal Science, https://reference-global.com/article/10.2478/aoas-2025-0086 (last access: 15 February 2026), 2025.
Krebs, I., Zhang, Y., Wente, N., Leimbach, S., and Krömker, V.: Severity of clinical mastitis and bacterial shedding, Pathogens, 12, 1098, https://doi.org/10.3390/pathogens12091098, 2023.
Kumari, K., Gupta, S. R., Singh, A., and Kachhawa, J.: Prevalence of subclinical mastitis in cattle in Bikaner, Prevalence, 13, 71–74, https://d1wqtxts1xzle7.cloudfront.net/121263586/2024_13_1_11._Kiran_Kumari_71_74_-libre.pdf (last access: 12 December 2025), 2024.
Leach, K. A., Archer, S. C., Breen, J. E., Green, M. J., Ohnstad, I. C., Tuer, S., and Bradley, A. J.: Recycling manure as cow bedding: Potential benefits and risks for UK dairy farms, Vet. J., 206, 123–130, https://doi.org/10.1016/j.tvjl.2015.08.013, 2015.
Li, H. and Sun, P.: Insight of melatonin: the potential of melatonin to treat bacteria-induced mastitis, Antioxidants, 11, 1107, https://doi.org/10.3390/antiox11061107, 2022.
Li, R., Zhang, C.-L., Liao, X.-X., Chen, D., Wang, W.-Q., Zhu, Y.-H., Geng, X.-H., Ji, D.-J., Mao, Y.-J., and Gong, Y.-C.: Transcriptome microRNA profiling of bovine mammary glands infected with Staphylococcus aureus, International journal of molecular sciences, 16, 4997–5013, https://doi.org/10.3390/ijms16034997, 2015.
Li, X., Xu, C., Liang, B., Kastelic, J. P., Han, B., Tong, X., and Gao, J.: Alternatives to antibiotics for treatment of mastitis in dairy cows, Frontiers in Veterinary Science, 10, 1160350, https://doi.org/10.3389/fvets.2023.1160350, 2023.
Ma, Y., Khan, M. Z., Xiao, J., Alugongo, G. M., Chen, X., Chen, T., Liu, S., He, Z., Wang, J., and Shah, M. K.: Genetic markers associated with milk production traits in dairy cattle, Agriculture, 11, 1018, https://doi.org/10.3390/agriculture11101018, 2021.
Maestroni, G. J.: Role of Melatonin in Viral, Bacterial and Parasitic Infections, Biomolecules, 14, 356, https://doi.org/10.3390/biom14030356, 2024.
Marichal, P., Gorrens, J., and Coene, M.: Biochemical basis for the activity and selectivity of oral antifungal drugs, Brit. J. Clin. Pract., 71, 41–46, https://europepmc.org/article/med/2091733 (last access: 12 December 2025), 1990.
Mbindyo, C. M., Gitao, G. C., and Mulei, C. M.: Prevalence, etiology, and risk factors of mastitis in dairy cattle in Embu and Kajiado Counties, Kenya, Veterinary Medicine International, 2020, 8831172, https://doi.org/10.1155/2020/8831172, 2020.
Melchior, M., Fink-Gremmels, J., and Gaastra, W.: Comparative assessment of the antimicrobial susceptibility of Staphylococcus aureus isolates from bovine mastitis in biofilm versus planktonic culture, J. Vet. Med. B, 53, 326–332, https://doi.org/10.1111/j.1439-0450.2006.00962.x, 2006.
Molineri, A. I., Camussone, C., Zbrun, M. V., Archilla, G. S., Cristiani, M., Neder, V., Calvinho, L., and Signorini, M.: Antimicrobial resistance of Staphylococcus aureus isolated from bovine mastitis: Systematic review and meta-analysis, Prev. Vet. Med., 188, 105261, https://doi.org/10.1016/j.prevetmed.2021.105261, 2021.
Moniri, R., Dastehgoli, K., and Akramian, A.: Increasing resistant coagulase negative Staphylococci in bovine clinical mastitis, Pakistan Journal of Biological Sciences, 10, 2465–2469, https://doi.org/10.3923/pjbs.2007.2465.2469, 2007.
More, S. J.: Global trends in milk quality: implications for the Irish dairy industry, Irish Vet. J., 62, S5, https://doi.org/10.1186/2046-0481-62-S4-S5, 2009.
Mudroňová, D., Karaffová, V., Pešulová, T., Koščová, J., Maruščáková, I. C., Bartkovský, M., Marcinčáková, D., Ševčíková, Z., and Marcinčák, S.: The effect of humic substances on gut microbiota and immune response of broilers, Food Agr. Immunol., 31, 137–149, https://doi.org/10.1080/09540105.2019.1707780, 2020.
Na, M.-J., Lee, W.-Y., and Park, H.-J.: Difenoconazole Induced Damage of Bovine Mammary Epithelial Cells via ER Stress and Inflammatory Response, Cells, 13, 1715, https://doi.org/10.3390/cells13201715, 2024.
Nale, J. Y. and McEwan, N. R.: Bacteriophage therapy to control bovine mastitis: A review, Antibiotics, 12, 1307, https://doi.org/10.3390/antibiotics12081307, 2023.
Nedić, S., Vakanjac, S., Samardžija, M., and Borozan, S.: Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus, Res. Vet. Sci., 125, 323–332, https://doi.org/10.1016/j.rvsc.2019.07.016, 2019.
Nemeth, J., Muckle, C., and Gyles, C.: In vitro comparison of bovine mastitis and fecal Escherichia coli isolates, Vet. Microbiol., 40, 231–238, https://doi.org/10.1016/0378-1135(94)90112-0, 1994.
Nickerson, S. and Ryman, V.: Role of Antibiotic Therapy in Mastitis Control for Lactating and Dry Cows, UGA Cooperative Extension Bulletin, 1516, 23–58, 2019.
Nickerson, S. C., Kautz, F. M., and Nace, E. L.: Managing mastitis in dairy heifers to improve overall herd health, https://openscholar.uga.edu/record/25160/files/NickersonHeiferMastitisBulletin.pdf (Last access: 5 March 2026), 2013.
Oltramari, C. E., da G. Pinheiro, M., de Miranda, M. S., Arcaro, J. R., Castelani, L., Toledo, L. M., Ambrósio, L. A., Leme, P. R., Manella, M. Q., and Júnior, I. A.: Selenium sources in the diet of dairy cows and their effects on milk production and quality, on udder health and on physiological indicators of heat stress, Ital. J. Anim. Sci., 13, 2921, https://doi.org/10.4081/ijas.2014.2921, 2014.
Otto, M.: Virulence factors of the coagulase-negative Staphylococci, Front. Biosci., 9, 841–863, https://doi.org/10.2741/1295, 2004.
Ozbey, G., Tanriverdi, E. S., Acik, M. N., Kalin, R., Otlu, B., and Zigo, F.: Carriage of antimicrobial resistance genes in Escherichia coli of bovine origin, Pol. J. Vet. Sci., 27, 537–546, https://doi.org/10.24425/pjvs.2024.151749, 2024.
Ozbey, G., Tanriverdi, E., and Zigo, F.: Prevalence and antimicrobial resistance profiles of bacterial pathogens associated with subclinical mastitis and dairy farm environments, Pol. J. Vet. Sci., 639–650, https://doi.org/10.24425/pjvs.2025.157278, 2025.
Pillai, V. P. S. and Reji, S. A.: The role of veterinary research in human health promotion in South Asia, Letters in Animal Biology, 3, 16–22, https://doi.org/10.62310/liab.v3i2.117, 2023.
Pitkälä, A., Haveri, M., Pyörälä, S., Myllys, V., and Honkanen-Buzalski, T.: Bovine mastitis in Finland 2001 – prevalence, distribution of bacteria, and antimicrobial resistance, J. Dairy Res., 87, 2433–2441, 2004.
Pontes, G. N., Cardoso, E. C., Carneiro-Sampaio, M. M., and Markus, R. P.: Injury switches melatonin production source from endocrine (pineal) to paracrine (phagocytes)–melatonin in human colostrum and colostrum phagocytes, J. Pineal Res., 41, 136–141, https://doi.org/10.1111/j.1600-079X.2006.00345.x, 2006.
Popescu, S., Borda, C., Diugan, E. A., Niculae, M., Stefan, R., and Sandru, C. D.: The effect of the housing system on the welfare quality of dairy cows, Ital. J. Anim. Sci., 13, 2940, https://doi.org/10.4081/ijas.2014.2940, 2014.
Preethirani, P., Isloor, S., Sundareshan, S., Nuthanalakshmi, V., Deepthikiran, K., Sinha, A. Y., Rathnamma, D., Nithin Prabhu, K., Sharada, R., and Mukkur, T. K.: Isolation, biochemical and molecular identification, and in-vitro antimicrobial resistance patterns of bacteria isolated from bubaline subclinical mastitis in South India, PLoS One, 10, e0142717, https://doi.org/10.1371/journal.pone.0142717, 2015.
Pyörälä, S. and Taponen, S.: Coagulase-negative Staphylococci – Emerging mastitis pathogens, Vet. Microbiol., 134, 3–8, https://doi.org/10.1016/j.vetmic.2008.09.015, 2009.
Rainard, P.: Mammary microbiota of dairy ruminants: fact or fiction?, Vet. Res., 48, 25, https://doi.org/10.1186/s13567-017-0429-2, 2017.
Rajkumar, S., Nayakvadi, S., Narnaware, S. D., Rajkumar, R. S., and Vithalrao, U. S. K.: Prevalence of Subclinical Mastitis, Associated Risk Factors and Pathogens in Dairy Cattle of West Coastal India, Indian Journal of Veterinary Sciences & Biotechnology, 20, 18, https://doi.org/10.48165/ijvsbt.20.5.18, 2024.
Ranjani, S., Priya, P. S., Veerasami, M., and Hemalatha, S.: Novel polyherbal nanocolloids to control bovine mastitis, Appl. Biochem. Biotech. 194, 246–265, https://doi.org/10.1007/s12010-021-03748-w, 2022.
Rather, I. A., Sabir, J. S., Asseri, A. H., Wani, M. Y., and Ahmad, A.: Triazole derivatives target 14α–demethylase (LDM) enzyme in Candida albicans causing ergosterol biosynthesis inhibition, Journal of Fungi, 8, 688, https://doi.org/10.3390/jof8070688, 2022.
Reiter, R. J., Mayo, J. C., Tan, D. X., Sainz, R. M., Alatorre-Jimenez, M., and Qin, L.: Melatonin as an antioxidant: under promises but over delivers, J. Pineal Res., 61, 253–278, https://doi.org/10.1111/jpi.12360, 2016.
Reitz, G. K. S., Pelegrini, M. M. B., Molinari, P. V., Londero, U. S., de Oliveira Feijó, J., Corrêa, M. N., Alvarado-Rincón, J. A., Gueretz, J. S., Peripolli, V., and Schwegler, E.: Subclinical mastitis in Jersey dairy cows and its effects on productivity and inflammatory markers, Semina: Ciências Agrárias, 45, 819–834, https://doi.org/10.5433/1679-0359.2024v45n3p819, 2024.
Reksen, O., Sølverød, L., Branscum, A., and Østerås, O.: Relationships between milk culture results and treatment for clinical mastitis or culling in Norwegian dairy cattle, J. Dairy Res., 89, 2928–2937, https://doi.org/10.3168/jds.S0022-0302(06)72565-6, 2006.
Ruegg, P. L.: A 100-Year Review: Mastitis detection, management, and prevention, J. Dairy Res., 100, 10381–10397, https://doi.org/10.3168/jds.2017-13023, 2017.
Ryman, V. E., Packiriswamy, N., and Sordillo, L. M.: Role of endothelial cells in bovine mammary gland health and disease, Anim. Health Res. Rev., 16, 135–149, https://doi.org/10.1017/S1466252315000158, 2015.
Sahoo, S., Behera, M. R., Mishra, B., Sahoo, P., and Kar, S.: Antibiotic-resistant bacteria in bovine milk in India, Journal of Advanced Veterinary and Animal Research, 10, 21, https://doi.org/10.5455/javar.2023.j648, 2023.
Sajib, M. M. R., Atiquzzaman, A., Devnath, B., Rahima, F. F., and Jalil, M. A.: Prevalence and associated risk factors of sub-clinical mastitis in lactating cows in selected area of Bangladesh, Research in Agriculture Livestock and Fisheries, 11, 271–281, https://doi.org/10.3329/ralf.v11i3.77753, 2024.
Samanta, S.: Physiological and pharmacological perspectives of melatonin, Arch. Physiol. Biochem., 128, 1346–1367, https://doi.org/10.1080/13813455.2020.1770799, 2022.
Sangiorgio, G., Calvo, M., Migliorisi, G., Campanile, F., and Stefani, S.: The impact of Enterococcus spp. in the immunocompromised host: a comprehensive review, Pathogens, 13, 409, https://doi.org/10.3390/pathogens13050409, 2024.
Schrick, F., Hockett, M., Saxton, A., Lewis, M., Dowlen, H., and Oliver, S.: Influence of subclinical mastitis 504 during early lactation on reproductive parameters, J. Dairy Sci., 84, 1407–1412, https://doi.org/10.3168/jds.S0022-0302(01)70172-5, 2001.
Seegers, H., Fourichon, C., and Beaudeau, F.: Production effects related to mastitis and mastitis economics in dairy cattle herds, Vet. Res., 34, 475–491, https://doi.org/10.1051/vetres:2003027, 2003.
Semjon, B., Marcinčáková, D., Koréneková, B., Bartkovský, M., Nagy, J., Turek, P., and Marcinčák, S.: Multiple factorial analysis of physicochemical and organoleptic properties of breast and thigh meat of broilers fed a diet supplemented with humic substances, Poultry Sci., 99, 1750–1760, https://doi.org/10.1016/j.psj.2019.11.012, 2020.
Shaheen, T., Ahmad, S. B., Rehman, M. U., Muzamil, S., Bhat, R. R., Hussain, I., Bashir, N., Mir, M. U. R., Paray, B. A., and Dawood, M. A.: Investigations on cytokines and proteins in lactating cows with and without naturally occurring mastitis, Journal of King Saud University - Science, 32, 2863–2867, https://doi.org/10.1016/j.jksus.2020.07.009, 2020.
Shahzad, M. A., Yousaf, A., Ahsan, A., Irshad, H., Riaz, A., Khan, A., Ullah, I., Sattar, S., Bostan, N., and Javed, S.: Virulence and resistance profiling of Staphylococcus aureus isolated from subclinical bovine mastitis in the Pakistani Pothohar region, Scientific Reports, 14, 14569, https://doi.org/10.1038/s41598-024-65448-9, 2024.
Sharma, N. and Jeong, D. K.: Stem cell research: a novel boulevard towards improved bovine mastitis management, Int. J. Biol. Sci., 9, 818, https://doi.org/10.7150/ijbs.6901, 2013.
Sharma, N., Singh, N., and Bhadwal, M.: Relationship of somatic cell count and mastitis: An overview, Asian Austral. J. Anim., 24, 429–438, https://doi.org/10.5713/ajas.2011.10233, 2011.
Smith, K. L., Todhunter, D., and Schoenberger, P.: Environmental mastitis: cause, prevalence, prevention, J. Dairy Res., 68, 1531–1553, https://doi.org/10.3168/jds.S0022-0302(85)80993-0, 1985.
Sol, J., Sampimon, O., Snoep, J., and Schukken, Y.: Factors associated with bacteriological cure after dry cow treatment of subclinical staphylococcal mastitis with antibiotics, J. Dairy Res., 77, 75–79, 1994.
Strydom, T., Lavan, R. P., Torres, S., and Pinilla, J. C.: The economic impact of endo-and ectoparasites in dairy cattle, Parasite. Vector., 18, 495, https://doi.org/10.1186/s13071-025-07064-8, 2025.
Sung, E., Park, J., Lee, H., Song, G., and Lim, W.: Bifenthrin induces cell death in bovine mammary epithelial cells via ROS generation, calcium ion homeostasis disruption, and MAPK signaling cascade alteration, Pestic. Biochem. Phys., 196, 105637, https://doi.org/10.1016/j.pestbp.2023.105637, 2023.
Tančin, V., Uhrincat, M., Macuhova, L., and Bruckmaier, R.: Effect of pre-stimulation on milk flow pattern and distribution of milk constituents at a quarter level, Czech J. Anim. Sci., 52, 117, https://doi.org/10.17221/2234-CJAS, 2007.
Tančin, V., Apolen, D., Botto, L., Brestenský, V., Brouček, J., and Dano, J.: Livestock farming in marginal areas, LuŽianky, Slovakia: CVŽV Nitra, 70, 2013.
Tiwari, J. G., Babra, C., Tiwari, H. K., Williams, V., De Wet, S., Gibson, J. S., Paxman, A., Morgan, E., Constantino, P., and Sunagar, R.: Trends intherapeutic and prevention strategies for management of bovine mastitis: An overview, Journal of Vaccines & Vaccination, 4, https://doi.org/10.4172/2157-7560.1000176, 2013.
Tomanić, D., Samardžija, M., and Kovačević, Z.: Alternatives to antimicrobial treatment in bovine mastitis therapy: a review, Antibiotics, 12, 683, https://doi.org/10.3390/antibiotics12040683, 2023a.
Tomanić, D., Samardžija, M., Kladar, N., Pećin, M., Ružić, Z., and Kovačević, Z.: Assessment of antibiotic use patterns in bovine mastitis treatment in the dairy sector in Serbia, Reprod. Domest. Anim., 58, 1756–1765, https://doi.org/10.1111/rda.14494, 2023b.
Tomanić, D., Samardžija, M., Stančić, I., Kladar, N., Maćešić, N., and Kovačević, Z.: Mastitis challenges in Serbian dairy farming: a study on somatic cell counts and pathogen distribution, Mljekarstvo: Dairy Experts Journal, 74, 239–248, https://doi.org/10.15567/mljekarstvo.2024.0307, 2024a.
Tomanić, D., Božić, D. D., Kladar, N., Samardžija, M., Apić, J., Baljak, J., and Kovačević, Z.: Clinical Evidence on Expansion of Essential Oil-Based Formulation's Pharmacological Activity in Bovine Mastitis Treatment: Antifungal Potential as Added Value, Antibiotics, 13, 575, https://doi.org/10.3390/antibiotics13070575, 2024b.
Toušová, R., Stádník, L., Ducháček, J., and Baráková, M.: Vliv vakcinace prvotelek proti mastitidě na její výskyt, počet somatických buněk a obsah pevných sloŽek v mléce/Influence of vaccination against mastitis on its incidence, somatic cells amount and content of solid components in milk of primiparous dairy cows, Výzkum v chovu skotu-Cattle Res., 53, 84–90, 2011.
Touza-Otero, L., Landin, M., and Diaz-Rodriguez, P.: Fighting antibiotic resistance in the local management of bovine mastitis, Biomed. Pharmacother., 170, 115967, https://doi.org/10.1016/j.biopha.2023.115967, 2024.
Trinidad, P., Nickerson, S., and Adkinson, R.: Histopathology of staphylococcal mastitis in unbred dairy heifers, J. Dairy Res., 73, 639–647, 1990.
Turk, R., Koledić, M., Maćešić, N., Benić, M., Dobranić, V., Đuričić, D., Cvetnić, L., and Samardžija, M.: The role of oxidative stress and inflammatory response in the pathogenesis of mastitis in dairy cows, Mljekarstvo: Dairy Experts Journal, 67, 91–101, https://doi.org/10.15567/mljekarstvo.2017.0201, 2017.
Turkyilmaz, S., Yildiz, O., Oryasin, E., Kaynarca, S., and Bozdogan, B.: Molecular identification of bacteria isolated from dairy herds with mastitis, Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16, https://doi.org/10.9775/kvfd.2010.2300, 2010.
Ulfman, L. H., Leusen, J. H., Savelkoul, H. F., Warner, J. O., and Van Neerven, R. J.: Effects of bovine immunoglobulins on immune function, allergy, and infection, Frontiers in Nutrition, 5, 52, https://doi.org/10.3389/fnut.2018.00052, 2018.
Vasiľ, M., Elečko, J., Zigo, F., and Farkašová, Z.: Occurrence of some pathogenity factors in coagulase negative Staphylococci isolated from mastitis milk in dairy cows, Slovak Journal of Food Sciences, https://doi.org/10.5219/186, 2012.
Vitenberga-Verza, Z., Pilmane, M., Šerstòova, K., Melderis, I., Gontar, Ł., Kochański, M., Drutowska, A., Maróti, G., and Prieto-Simón, B.: Identification of inflammatory and regulatory cytokines IL-1α-, IL-4-, IL-6-, IL-12-, IL-13-, IL-17A-, TNF-α-, and IFN-γ-producing cells in the milk of dairy cows with subclinical and clinical mastitis, Pathogens, 11, 372, https://doi.org/10.3390/pathogens11030372, 2022.
Webster, J.: Understanding the dairy cow, John Wiley & Sons, ISBN: 9781119550242, 2020.
White, R., Abdulla, Z., Toni, F., Lemarchand, F., Miciletta, M., De Prado, A., Crawshaw, M., and Clemence, R.: Effect of introducing OrbeSeal into a dry cow management programme and the impact on farm economics, in: Udder health and communication, Wageningen Academic, 382–382, https://doi.org/10.3920/9789086867424_074, 2011.
Williamson, J., Woolford, M., and Day, A.: The prophylactic effect of a dry-cow antibiotic against Streptococcus uberis, New Zeal. Vet. J., 43, 228–234, https://doi.org/10.1080/00480169.1995.35898, 1995.
Xu, L., Zhang, W., Kwak, M., Zhang, L., Lee, P. C., and Jin, J.-O.: Protective effect of melatonin against polymicrobial sepsis is mediated by the anti-bacterial effect of neutrophils, Front. Immunol., 10, 1371, https://doi.org/10.3389/fimmu.2019.01371, 2019.
Yalcin, S., Ozgen, A., and Simsir, M.: Molecular characteristics and antimicrobial susceptibility profiles of bovine mastitis agents in western Türkiye, J. Vet. Sci., 25, e72, https://doi.org/10.4142/jvs.24032, 2024.
You, H., An, G., Lee, H., Lim, W., and Song, G.: Bifenox induces programmed cell death in bovine mammary epithelial cells by impairing calcium homeostasis, triggering ER stress, and altering the signaling cascades of PI3K/AKT and MAPK, Pestic. Biochem. Phys., 196, 105626, https://doi.org/10.1016/j.pestbp.2023.105626, 2023.
Youssif, N., Hafiz, N., Halawa, M., Aziz, H., and Saad, M.: Impact of subclinical mastitis on milk quality in different seasons, 15, 9785, Acta Veterinaria Brasilica, https://doi.org/10.21708/avb.2021.15.2.9785, 2020.
Zadoks, R. and Fitzpatrick, J.: Changing trends in mastitis, Irish Vet. J., 62, S59, https://doi.org/10.1186/2046-0481-62-S4-S59, 2009.
Zahoor, A., Yang, Y., Yang, C., Khan, S. B., Reix, C., Anwar, F., Guo, M.-y., and Deng, G.: MerTK negatively regulates Staphylococcus aureus induced inflammatory response via Toll-like receptor signaling in the mammary gland, Mol. Immunol., 122, 1–12, https://doi.org/10.1016/j.molimm.2020.03.007, 2020.
Zajác, P., Tomáška, M., Murárová, A., Čapla, J., and Čurlej, J.: Quality and safety of raw cow's milk in Slovakia in 2011, Slovak Journal of Food Sciences, https://doi.org/10.5219/189, 2012.
Zaman Faruk, M. A., Manu, M. M. R., Afroz, F., Ali, M. W., Haque, M. A., Danishuddin, and Haque, M. A.: Prevalence, Risk Factors, and Antibiogram Analysis of Bovine Mastitis in Northern Bangladesh, Veterinary Sciences, 12, 1201, https://doi.org/10.3390/vetsci12121201, 2025.
Zefferino, R., Di Gioia, S., and Conese, M.: Molecular links between endocrine, nervous and immune system during chronic stress, Brain Behav., 11, e01960, https://doi.org/10.1002/brb3.1960, 2021.
Zhang, H., Jiang, H., Fan, Y., Chen, Z., Li, M., Mao, Y., Karrow, N. A., Loor, J. J., Moore, S., and Yang, Z.: Transcriptomics and iTRAQ-proteomics analyses of bovine mammary tissue with Streptococcus agalactiae-induced mastitis, J. Agr. Food Chem., 66, 11188–11196, https://doi.org/10.1021/acs.jafc.8b02386, 2018.
Zhang, T., Qin, J., Zhu, B., Wang, Y., Liu, Q., Fang, J., Zhang, S., Deng, S., Chen, J., and Zhang, Y.: Topical VEGF-A mRNA therapy promotes angiogenesis and accelerates wound healing, Cell Investigation, 1, 100043, https://doi.org/10.1016/j.clnves.2025.100043, 2025.
Zhang, Z., Chen, Y., Li, X., Wang, X., and Li, H.: Detection of antibiotic resistance, virulence gene, and drug resistance gene of Staphylococcus aureus isolates from bovine mastitis, Microbiology Spectrum, 10, e00471-00422, https://doi.org/10.1128/spectrum.00471-22, 2022.
Zhylkaidar, A., Oryntaev, K., Altenov, A., Kylpybai, E., and Chayxmet, E.: Prevention of bovine mastitis through vaccination, Archives of Razi Institute, 76, 1381, https://doi.org/10.22092/ari.2021.356008.1764, 2021.
Zi, C., Zeng, D., Ling, N., Dai, J., Xue, F., Jiang, Y., and Li, B.: An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR, BMC Microbiology, 18, 1–8, https://doi.org/10.1186/s12866-018-1273-x, 2018.
Zigo, F., Farkasova, Z., Lapin, M., Chripková, M., and Czerski, A.: Effect of parenteral administration of Selenium and vitamin E on health status of mammary gland and on selected antioxidant indexes in blood of dairy cows, Pol. J. Vet. Sci., https://doi.org/10.2478/pjvs-2014-0031, 2014.
Zigo, F., Elečko, J., Farkašová, Z., Zigová, M., Vasiľ, M., Ondrašovičová, S., and Lenka, K.: Preventive methods in reduction of mastitis pathogens in dairy cows, Journal of Microbiology, Biotechnology and Food Sciences, 9, 121–126, https://doi.org/10.15414/jmbfs.2019.9.1.121-126, 2019.
Zigo, F., Sasáková, N. a., Gregová, G., Výrostková, J., and Ondrašovičová, S.: Effects of using an alternative bedding composition on the levels of indicator microorganisms and mammary health in dairy farm conditions, Agriculture, 10, 245, https://doi.org/10.3390/agriculture10060245, 2020.
Zigo, F., Vasil', M., Ondrašovičová, S., Výrostková, J., Bujok, J., and Pecka-Kielb, E.: Maintaining optimal mammary gland health and prevention of mastitis, Frontiers in Veterinary Science, 8, 607311, https://doi.org/10.3389/fvets.2021.607311, 2021.
Zigo, F., Farkašová, Z., Výrostková, J., Regecová, I., Ondrašovičová, S., Vargová, M., Sasáková, N., Pecka-Kielb, E., Bursová, Š., and Kiss, D. S.: Dairy cows' udder pathogens and occurrence of virulence factors in Staphylococci, Animals, 12, 470, https://doi.org/10.3390/ani12040470, 2022.
Short summary
Bovine mastitis, a costly inflammation caused mainly by pathogenic bacteria, harms milk yield and the economy worldwide. This review covers its clinical forms, diagnostics, the immune role of melatonin, and alternatives to antibiotics (such as herd management and bacteriophages). It highlights research gaps in genetics and microbiomes, urging integrated, sustainable control strategies.
Bovine mastitis, a costly inflammation caused mainly by pathogenic bacteria, harms milk yield...