Articles | Volume 68, issue 3
https://doi.org/10.5194/aab-68-555-2025
https://doi.org/10.5194/aab-68-555-2025
Original study
 | 
11 Sep 2025
Original study |  | 11 Sep 2025

Non-steroidal anti-inflammatory drugs improve bovine blastocyst formation and quality in in vitro culture

Kübra Karakaş Alkan, Fatma Satılmış, Yunus Emre Deniz, Mustafa Bodu, Muhammed Furkan Çiftçi, Ömer Faruk Yeşilkaya, and Hasan Alkan

Related authors

Effect on OPU/IVEP success of different applications for synchronizing follicular waves prior to superstimulation in holstein heifers
Muhammed Furkan Çiftçi, Ömer Faruk Yeşilkaya, Sakine Ülküm Çizmeci, Maide Gölbaşı, Ayşe Sarı, and Dursun Ali Dinç
Arch. Anim. Breed., 68, 101–107, https://doi.org/10.5194/aab-68-101-2025,https://doi.org/10.5194/aab-68-101-2025, 2025
Short summary

Cited articles

Aguila, L., Osycka-Salut, C., Treulen, F., and Felmer, R.: Pluripotent Core in Bovine Embryos: A Review, Animals, 12, 1010, https://doi.org/10.3390/ani12081010, 2022. 
Alkan, H., Satilmis, F., Demirel, M. A., Bodu, M., Yesilkaya, O. F., Ciftci, M. F., Erdem, H., Tekindal, M. A., and Alkan, K. K.: Does Using Microfluidic Sperm Sorting Chips in Bovine IVEP Affect Blastocyst Development?, Reprod. Domest. Anim., 58, 1012–1020, https://doi.org/10.1111/rda.14398, 2023. 
Antunes, G., Chaveiro, A., Santos, P., Marques, A., Jin, H. S., and da Silva, F. M.: Influence of Apoptosis in Bovine Embryo's Development, Reprod. Domest. Anim., 45, 26–32, https://doi.org/10.1111/j.1439-0531.2008.01131.x, 2010. 
Bicici, E., Satilmis, F., Bodu, M., Demirel, M. A., Karakas Alkan, K., and Alkan, H.: Effect of putrescine supplementation to in vitro maturation medium on embryo development and quality in cattle, Anim. Biotechnol., 34, 3887–3896, https://doi.org/10.1080/10495398.2023.2236660, 2023. 
Bó, G. A. and Mapletoft, R. J.: Evaluation and Classification of Bovine Embryos, Anim. Reprod., 10, 344–348, 2013. 
Download
Short summary
Prostaglandins have a detrimental effect on embryo development during the early phase of in vitro culture. In this study, non-steroidal anti-inflammatory drugs (NSAIDs) were supplemented in the in vitro culture medium to eliminate the negative effect of prostaglandins during in vitro embryo culture, and the effect of these NSAIDs on embryo development and quality was evaluated. Embryos were assessed on day 7 based on developmental stage, quality, apoptotic index, and developmental competence.
Share