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Articles | Volume 65, issue 1
Articles | Volume 65, issue 1
https://doi.org/10.5194/aab-65-47-2022
© Author(s) 2022. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/aab-65-47-2022
© Author(s) 2022. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 65, issue 1
Original study
 | 
07 Feb 2022
Original study |  | 07 Feb 2022

Allele-specific polymerase chain reaction for the discrimination of elite Korean cattle associated with high beef quality and quantity

Wonhee Lee, Insik Nam, Daehyun Kim, Kukdong Kim, and Yoonseok Lee

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Latest update: 03 Nov 2025
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Short summary
The AS-PCR analyzes genotypes using two pairs of primer in basic equipment: a thermal cycler and an electrophoresis system. This research team analyzed three SNPs in 10 Korean cattle using the AS-PCR method. The result can be easily confirmed visually through the appearance of different amplification sizes depending on allele. Therefore, the use of the AS-PCR method will enable researchers to carry out genotyping analysis for SNPs without the use of sophisticated instrumentation and at low cost.
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