The study was to clarify the roles of ACSL5 in regulating synthesis of triglycerides and lipid droplet formation in bovine preadipocytes through vectors constructing and electransfection. Research results confirmed that the ACSL5 gene could promote the synthesis of triglycerides in bovine adipocytes by participating in the fatty acid metabolism pathway. The interaction of ACSL5 with related fatty acid synthase in the fatty acid biosynthesis pathway needs further analysis.

ASIP can regulate the obesity of mice but studies of ASIP effects on bovine fat deposition and meat quality are rare. The L1-BT element inserted upstream of bovine ASIP can cause ASIP mRNA overexpression. In this study, L1-BT element indel, two single nucleide polymorphisms (SNPs) in ASIP, and its haplotypes showed significant correlations with fat deposition traits and fatty acid composition in Chinese Simmental steers, suggesting that ASIP may be a biomarker for beef cattle breeding selection.