Effects of in-yolk-sac administration of carvacrol on cholesterol resorption from yolk residuals and physiological adaptive indicators in broiler chicks exposed to neonatal fasting
- 1Department of Animal Science, Faculty of Agriculture, Lorestan University, Khorramabad, Lorestan, Iran
- 2Department of Pathology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Abstract. Three hundred and twenty Ross 308 male broiler chicks were used to examine effects of in-yolk-sac (IYS) administration of carvacrol on cholesterol resorption from yolk and physiological adaptive responses of broiler chicks subjected to post-hatch fasting periods of up to 72 h. Effects of the four experimental treatments, namely non-handled control (NHCON), sham injection control (SICON), polysorbate-80 injection (POLS), and carvacrol injection (CARV), were examined in 5 replicates of 10 birds each. Liver proportional weight was greater in carvacrol-injected chicks compared with other birds 24 h post-hatch (P < 0.05). The mean blood glucose concentration was 199.0 mg dL−1 when chicks were removed from the hatcher baskets, and decreased in all birds after being subjected to a 72 h post-hatch fasting. However, the slope of decrease in serum concentration of glucose was slower in carvacrol-injected birds than in the other birds, and they had a greater plasma glucose level compared with NHCON and SICON birds after 72 h post-hatch fasting. Lower plasma cholesterol levels were observed in carvacrol-treated chicks compared with those subjected to the other treatments at 72 h post-hatch (P < 0.05). Blood concentration of calcium (Ca) was greater in carvacrol-injected birds at 24 h post-hatch than in NHCON and SICON birds (P < 0.05), but at 72 h it significantly increased in all birds, with the exception of carvacrol-treated chicks, which had significantly lower blood Ca concentration (11.17 mg dL−1) compared with other birds (P < 0.05). Blood potassium concentration increased in polysorbate-80 and carvacrol-injected chicks 24 h post-hatch compared with the NHCON and SICON birds (P < 0.05). In conclusion, the results of the current study revealed that there was no direct interaction between cholesterol and carvacrol leading to reduced cholesterol absorption from yolk sac in newly hatched broiler chicks.