A novel EcoRII PCR-RFLP detecting genetic variation of goat NF1-C2 gene and its association with milk yield (Brief Report)

Abstract. The transcription factor nuclear factor1-C2 (NF1-C2) mediates the action of prolactin in the mammary gland. Research on the molecular genetic mechanism of model system have indicated that the NF1-C2 gene plays an important role for the activation of several mammary gland specific genes (Nilsson et al. 2006). Moreover, the effects of prolactin on milk production traits have been reported in ruminant (Seriwatanachai et al. 2008). NF1-C2 may have a similar function in goat. To our knowledge polymorphisms of NF1-C2 gene have not been described in animals. Here a SNP of the caprine NF1-C2 was detected and a EcoRII PCR-RFLP was derived, that allowed to clearly detect NF1-C2 genotypes. Further investigation was conduct to evaluate the association between polymorphisms and milk yield at different lactation stages.