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Volume 51, issue 3
Arch. Anim. Breed., 51, 298–299, 2008
https://doi.org/10.5194/aab-51-298-2008
© Author(s) 2008. This work is distributed under
the Creative Commons Attribution 3.0 License.
Arch. Anim. Breed., 51, 298–299, 2008
https://doi.org/10.5194/aab-51-298-2008
© Author(s) 2008. This work is distributed under
the Creative Commons Attribution 3.0 License.

  10 Oct 2008

10 Oct 2008

Genotyping of the polymorphism within exon 3 of prolactin gene in various dairy breeds by PCR RFLP (Brief report)

A. Ratna Kumari1, K. M. Singh2, K. J. Soni2, R. K. Patel2, J. B. Chauhan2, and Krs Sambasiva Rao1 A. Ratna Kumari et al.
  • 1Centre for Biotechnology, Acharya Nagarjuna University, Nagarjunanagar, Guntur-522 510, India
  • 2R&D-Biotechnology, National Dairy Development Board, Anand-388 001, India

Abstract. In mammals, especially dairy cattle the prolactin has important functions like the development of mammary gland affecting milk yield and composition. It has been mapped to chromosome 23 in Bovine (HALLERMAN et al., 1988). A silent A→G transition mutation at the codon for amino acid 103 in exon 3 of bovine prolactin (bPRL) gene gives rise to a polymorphic Rsa I site, has become a popular genetic marker used for genetic characterization of cattle populations by means of PCR-RFLP (MITRA et al., 1995; CHRENEK et al., 1998; DYBUS, 2005). The present study reports on the genotype frequencies observed in various Bos taurus and Bos indicus dairy cattle breeds.

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