Individual and combined effects of CAPN1, CAST, LEP and GHR gene polymorphisms on carcass characteristics and meat quality in Holstein bulls

Abstract. The objective of this study was to determine the association of single nucleotide polymorphisms (SNPs) with carcass characteristics and meat quality traits in selected candidate genes in Holstein bulls. Five SNPs in four genes, i.e. calpain 1 (CAPN1), calpastatin (CAST), leptin (LEP) and growth hormone receptor (GHR), were genotyped in 400 purebred bulls using PCR-RFLP. Statistically significant associations were as follows: CAPN1 G316A with live weight, carcass weight, back fat thickness, m. longissimus thoracis et lumborum area and carcass measurements; CAPN1 V530I with pH and L^∗; CAST S20T with live weight, inner chest depth and b^∗ value; and GHR with ph, a^∗ and h^∗. In addition, significant genotypic interactions were observed for dressing percentage (LEP A80V  ×  CAST S20T), pH (CAPN1 V530I  ×  GHR S555G and LEP A80V  ×  GHR S555G) and rump width (CAPN1 V530I  ×  CAST S20T). There was no association between the LEP A80V marker and any of the traits evaluated, nor was there any association of the tested SNPs with chest width, C^∗ and marbling score. The present results could therefore be indicative for future studies on meat yield and quality.