Articles | Volume 58, issue 2
Arch. Anim. Breed., 58, 445–449, 2015
https://doi.org/10.5194/aab-58-445-2015
Arch. Anim. Breed., 58, 445–449, 2015
https://doi.org/10.5194/aab-58-445-2015

  04 Dec 2015

04 Dec 2015

Molecular cloning, sequence identification, polymorphism and association of the porcine SPATS2L gene

D. Yang1,2 and Y. Liu2 D. Yang and Y. Liu
  • 1Yunnan Key Laboratory of Fertility Regulation and Minority Eugenics, Kunming, China
  • 2Key Laboratory of Animal Nutrition and Feed of Yunnan Province, Yunnan Agricultural University, Kunming, China

Abstract. Spermatogenesis-associated, serine-rich 2-like (SPATS2L) is an important reproduction-related gene. In this study, the full-length cDNA sequence of the porcine SPATS2L gene was cloned through the RACE (rapid amplification of cDNA ends) method. The porcine SPATS2L gene encodes a protein of 559 amino acids which shares high homology with the SPATS2L proteins of seven species: dog (94 %), white-tufted-ear marmoset (91 %), human (92 %), rhesus monkey (92 %), horse (92 %), rat (88 %) and mouse (88 %). This novel porcine gene was assigned to GeneID: 100415809. The phylogenetic analysis revealed that the porcine SPATS2L gene has a close genetic relationship with the canine SPATS2L gene. PCR-Pst I-RFLP was established to detect GU474997:c.1687 C > T substitution of porcine SPATS2L gene mRNA, and eight pig breeds displayed obvious genotype and allele frequency differences at this mutation locus. Association of this single-nucleotide polymorphism (SNP) with litter size traits was assessed in Large White (n = 100) and Landrace (n = 100) pig populations, and results demonstrated that this polymorphic locus was significantly associated with the litter size of all parities in Large White sows and Landrace sows (P < 0.01). Therefore, SPATS2L gene could be an useful candidate gene in selection for increasing litter size in pigs.

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