Genetic admixture analysis in Prestice Black-Pied pigs
Abstract. The Prestice Black-Pied pig (PC) is a genetic resource of the Czech Republic. Information about boar variability has been provided in preceding papers. The objective of this work was to compare genetic variability of boars and sows. A total of 21 microsatellite markers were used to study genetic diversity and population structure of 149 PC breeding animals. In this paper, current parameters of assessments of diversity in PC are listed. All loci were polymorphic, and a total of 167 alleles were observed across the studied loci. The range of alleles was found to be 4 to 15 with a mean of 7.95. Overall allele frequency ranged from 0.07 to 0.25. The calculated observed and expected heterozygosity values were 0.70 and 0.73, respectively. The polymorphism information content (PIC) was 0.69 and the overall within-population inbreeding estimate (FIS) was 0.04. Genetic differentiation and population subdivision were tested. The level of admixture of breeds used for improvement of the PC breed was evaluated (Landrace, Large White and Pietrain). Five percent of analysed PC breeding boars had more than 20% admixture of other monitored breeds. The obtained results are important for the future sustainability of this local breed.
