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Volume 57, issue 1
Arch. Anim. Breed., 57, 25, 2014
© Author(s) 2014. This work is distributed under
the Creative Commons Attribution 3.0 License.
Arch. Anim. Breed., 57, 25, 2014
© Author(s) 2014. This work is distributed under
the Creative Commons Attribution 3.0 License.

  29 Oct 2014

29 Oct 2014

In-vitro antibacterial sensitivity of Usnea barbata lichen extracted with methanol and ethyl-acetate against selected Staphylococcus species from milk of cows with mastitis

Emrobowansan Monday Idamokoro1, Patrick Julius Masika2, Voster Muchenje1, Daniel Falta3, and Ezekiel Green4 Emrobowansan Monday Idamokoro et al.
  • 1Department of Livestock and Pasture
  • 2Agricultural and Rural Development Research Institute
  • 3Department of Animal Breeding, Faculty of Agronomy, Mendel University in Brno, Czech Republic
  • 4Microbial Pathogenicity and Molecular Epidemiology Research Group, Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, South Africa

Abstract. This study aimed at evaluating the antimicrobial potential of Usnea barbata lichen as a medicinal plant against selected Staphylococcus species isolated from raw milk of cows. In-vitro screening of methanol and ethyl-acetate extracts from Usnea barbata lichen were evaluated to determine their antimicrobial activity against thirteen different Staphylococcus species. The selected organisms were isolated from raw bovine milk and identified using several biochemical tests and confirmed with API staph kit. The antimicrobial activity of the extracts were evaluated using both the agar well diffusion method (at 5 mg/ml, 10 mg/ml and 20 mg/ml) and the broth micro-dilution technique to determine the mean zone of inhibition and the minimum inhibitory concentration (MIC), respectively. Both the methanol and ethyl-acetate extracts showed variable antimicrobial activity against the Staphylococcus species with mean zones of inhibition ranging from 0-34 mm in diameter at 5 mg/ml, 10 mg/ml and 20 mg/ml, respectively. Susceptibility by the Staphylococcus species tested in the methanol and the ethyl-acetate extract was 92.31 % and 53.85 %, respectively. The MIC result for the methanol extract ranged from 0.04 to 10 mg/ml, while that of the ethyl-acetate extract ranged from 0.16 to 5 mg/ml. Results from this study revealed the in vitro microbial activity of Usnea barbata extracts which indicate its potential as a medicinal plant.