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Volume 57, issue 1
Arch. Anim. Breed., 57, 13, 2014
https://doi.org/10.7482/0003-9438-57-013
© Author(s) 2014. This work is distributed under
the Creative Commons Attribution 3.0 License.
Arch. Anim. Breed., 57, 13, 2014
https://doi.org/10.7482/0003-9438-57-013
© Author(s) 2014. This work is distributed under
the Creative Commons Attribution 3.0 License.

  23 May 2014

23 May 2014

Comparison of different chromatin staining techniques for bull sperm

Katarzyna Andraszek1, Dorota Banaszewska2, Magdalena Czubaszek1, Ewa Wójcik1, and Małgorzata Szostek1 Katarzyna Andraszek et al.
  • 1Department of Animal Genetics and Horse Breeding
  • 2Department of Breeding Methods and Poultry and Small Ruminant Breeding, Institute of Bioengineering and Animal Breeding, University of Natural Sciences and Humanities, Siedlce, Poland

Abstract. Morphological analysis of semen is a very important step in fertility assessment, but many semen defects are not detectable at the morphological level. These include pathological changes in sperm chromatin structure. During mammalian spermiogenesis, histone proteins associated with DNA structure are replaced by specific protamines, with which chromatin does not form nucleosomal complexes. In the fully developed, mature sperm, the histones are replaced with protamines. Disruptions of nucleoprotein structure can be treated as possible indicators of the biological value of spermatozoa. The experimental material consisted of sperm from one-and-a-half-year-old bulls, isolated post mortem from the tail of the epididymis. The smears were stained with silver nitrate (AgNO3), acridine orange (AO), aniline blue (AB) and chromomycin A3 (CMA3). Sperm dimensions largely depend on individual variability among the bulls. In most cases, differences in sperm dimensions were identified between individuals, which was confirmed in the statistics. Sperm with elevated, abnormal histone levels were proportionally quite scarce (1.4 %). Studies of nuclear proteins in the context of infertility demonstrate the important influence of normal chromatin structure on sperm functions.

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