Articles | Volume 54, issue 1
https://doi.org/10.5194/aab-54-18-2011
https://doi.org/10.5194/aab-54-18-2011
10 Oct 2011
 | 10 Oct 2011

Assessment of haplotype variation in bovine AMPD1 gene for association with growth and carcass traits in Qinchuan beef cattle

H. He, X. Liu, Y. Gu, and Y. Liu

Abstract. The AMPD1 gene plays an important role in the purine nucleotide cycle and energy metabolism in skeletal muscle. In the present study polymorphisms of the AMPD1 gene were detected by PCR-SSCP and DNA sequencing of 215 individuals of the Qinchuan beef cattle breed. DNA sequencing revealed two mutations by comparisons with the bovine genome sequence (acc. no.: NC_007301). Two single nucleotide polymorphisms (SNPs; g.19416T>C and g.19421A>G) were detected in intron 11 of the bovine AMPD1 gene. The sequencing of PCR products of animals providing different PCR-SSCP banding patterns showed that four kinds of haplotypes, named: A (T-A), B (T-G), C (C-A) and D (C-G); and the five diplotypes were segregating: AA (T-A/T-A), BC (T-G/C-A or C-G/T-A ), AC (T-A/C-A), CC (C-A/C-A) and CD (C-A/ C-G). A significant association of AMPD1 with carcass weight was shown. Animals with the new heterozygote diplotype BC (P<0.05, n=56) had greater carcass weight than those with the other diplotypes. The SNPs in AMPD1 may be used as a possible candidates for markerassisted selection in Qinchuan beef cattle breeding program.