Archives Animal Breeding
Archives Animal Breeding
AAB
Articles | Volume 53, issue 5
Articles | Volume 53, issue 5
https://doi.org/10.5194/aab-53-618-2010
© Author(s) 2010. This work is distributed under
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/aab-53-618-2010
© Author(s) 2010. This work is distributed under
the Creative Commons Attribution 3.0 License.
Articles | Volume 53, issue 5
10 Oct 2010
 | 10 Oct 2010

Isolation of 11 new polymorphic microsatellites from CA enriched turkey genomic libraries (Short communication)

E. Korom, K. Bakos, G. Veress, O. Pinke, K. M. Reed, L. Varga, and B. Kovács

Abstract. Microsatellite loci from the ancient Hungarian variety of the Broad Breasted Bronze Turkey (Meleagris gallopavo) were isolated. CA-repeat enriched libraries were constructed from DNA of randomly collected samples. Libraries were screened for repeat-containing clones by PIMA (PCR Isolation of Microsatellite Arrays) and the DNA-sequence of 167 positive clones was determined. A total of 136 microsatellite repeat-containing sequences were found, 59 sequences were unique. Comparing these with the genomic databases, we found 7 previously annotated microsatellite sequences. The newly isolated 52 microsatellites were tested on the mapping population of the University of Minnesota, and the map position of 11 microsatellites was determined.

How to cite. Korom, E., Bakos, K., Veress, G., Pinke, O., Reed, K. M., Varga, L., and Kovács, B.: Isolation of 11 new polymorphic microsatellites from CA enriched turkey genomic libraries (Short communication), Arch. Anim. Breed., 53, 618–622, https://doi.org/10.5194/aab-53-618-2010, 2010.