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Volume 53, issue 3
Arch. Anim. Breed., 53, 277–282, 2010
https://doi.org/10.5194/aab-53-277-2010
© Author(s) 2010. This work is distributed under
the Creative Commons Attribution 3.0 License.
Arch. Anim. Breed., 53, 277–282, 2010
https://doi.org/10.5194/aab-53-277-2010
© Author(s) 2010. This work is distributed under
the Creative Commons Attribution 3.0 License.

  10 Oct 2010

10 Oct 2010

Polymorphism of the kappa-casein gene in two Bosnian autochthonous cattle breeds

M. Brka1, A. Hodžić3, N. Reinsch2, E. P. Zečević1, A. Dokso1, R. Djedović3, D. Rukavina3, L. Kapur4, M. Vegara5, M. Šabanović6, and I. Ravić6 M. Brka et al.
  • 1Institute of Animal Breeding, Faculty of Agriculture and Food Sciences, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
  • 2Leibniz Institute for Farm Animal Biology, Dummerstorf, Germany
  • 3Department of Morphology, Veterinary Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
  • 4Institute for Genetic Engineering and Biotechnology, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
  • 5Department of International Environment and Development Studies, Norwegian University of Life Science, Aas, Norway
  • 6Veterinary service, Široki Brijeg, Bosnia and Herzegovina

Abstract. Buša is an old endangered autochthonous breed of the western Balkan, especially Bosnia-Herzegovina, Kosovo and Albania. A related breed is Gatačko, derived from Buša × Tirolean Grey crossbreds. Fifteen purebred Buša cattle and thirteen Gatačko animals were genotyped for polymorphisms at the kappa-casein gene by a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) essay. The alleles A, B and C were found and the allelic frequencies were 0.46 (A), 0.46 (B) and 0.08 (C) in Buša cattle and 0.58 (A) and 0.42 (B) in Gatačko. Only AA, AB, BB and BC genotypes occurred. Further alleles were not detected and are therefore either absent in both populations or rare. The allele »B« found in this small population will be useful for a sire selection program in the future.

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