The influence of vaccum pressure on quality and number of recovered oocytes aspirated from ovarian follicles of swine and cows
Abstract. The aim of the present study was stating the influence of the value of vaccum pressure on the percentage of oocytes aspirated from follicles, as well as their quality in heifers, cows, HF, sows and gilts crossbred Landrace. Follicles of >2 mm diameter were aspirated. The follicle liquid was obtained with application of the pressure equal to respectively –150, –100, –70, –50, –30 mm Hg respectively. Mean number of recovered oocytes in heifers and cows was respectively 13.3 and 8.4, while sows and gilts 27.5 and 16.3. An average number of the obtained oocytes in heifers and cows reached 61.42% and 48.9% and was not dependent on the size of the flow. The vaccum pressure value did significantly influence the number of aspirated oocytes of very good, sufficient and insufficient quality in heifers, as well as oocytes of sufficient and insufficient quality in cows. Alongside with an increase of vaccum pressure above 50 and 70 mm Hg in correspondingly heifers and cows, the percentage of insufficient quality oocytes increased while the percentage of very good quality oocytes decreased. An average percentage of recovered oocytes of gilts and sows equalled to 79.1 and 85.2% and did not depend on the size of the flow. In both gilts and sows the value of the vaccum pressure significantly influenced the number of the recovered oocytes of all classes. Alongside with the growth of the pressure above 50mm Hg the percentage of insufficient quality oocytes increased, while the percentage of very good and good quality oocytes decreased.