Articles | Volume 48, issue 5
Arch. Anim. Breed., 48, 505–517, 2005
https://doi.org/10.5194/aab-48-505-2005
Arch. Anim. Breed., 48, 505–517, 2005
https://doi.org/10.5194/aab-48-505-2005

  10 Oct 2005

10 Oct 2005

Skeletal muscle and liver protein degradation in mice divergently selected for low and high body weight over 108 generations

S. J. Rosochacki1,2, E. Wirth-Dzięciołowska3, M. Zimowska4, T. Sakowski1, J. Połoszynowicz1, E. Juszczuk-Kubiak1, and M. Gajewska5 S. J. Rosochacki et al.
  • 1Polish Academy of Sciences, Institute of Genetics and Animal Breeding, Jastrzębiec, 05-552 Wólka Kosowska, Poland
  • 2Białystok Technical University, Chair of Sanitary Biology and Biotechnology, 15-351 Białystok, Poland
  • 3Warsaw Agricultural University, Department of Genetics and Animal Breeding, 02-786 Warszawa, Poland
  • 4Warsaw University, Faculty of Biology, Institute of Zoology, Department of Cytology, 02-096 Warszawa, Poland
  • 5Museum and Institute of Zoology, 00-679 Warszawa, Poland

Abstract. Experiment was carried out on 111 and 13-14 weeks old mice divergently selected for low (L) and high (C) body weight over 108 generations. In leg muscle and liver cathepsin D (CatD) was determined as pepstatin sensitive activity (PSCatD), and as pepstatin-insensitive (PIA) and leupeptin-insensitive (LIA) acid autolytic activities (AAA) were measured. In leg muscle and liver all measured activities were higher in L as compared to C and control (K) mice. The percent of inhibition of LIA was lower in L then C mice in both examined tissues. In the leg muscles, in L mice protein catabolism was mostly influenced by CatD, but in the liver CatD was mostly active in K mice. CatD, PSCatD, AAA and LIA in leg muscle of L mice as compare to K were higher in female (F) by 68 %, 68.5 %, 91.3 % and 94.5 %, respectively, and in male (M) mice by 43.4 %, 54 %, 47.1 % and 64 %, respectively. The percent of inhibition by leupeptin in AAA was higher in C mice by 61.9 % in F and by 40 % in M mice. In the liver, PIA was higher by about 45 % in L and by 28.5 % in C mice as compare to K ones. There were about 36% of 30.00-39.99 μm muscle fibrils in M mice and in males of L group 40.00-49.99 μm fibrils appeared at 25% (absent in 3 weeks mice). In female, all measured fibril diameters were in higher percent in L than in C group. RNA variables were higher by about 25% (in average) in C as compare to K and L groups. Functional cell size (FCS) was lowered in L and C group as compared to K by about 10 % (in average). These results indicate on faster proteins turnover in L than in C group of mice.