Journal cover Journal topic
Archives Animal Breeding Archiv Tierzucht
Journal topic

Journal metrics

IF value: 0.991
IF0.991
IF 5-year value: 1.217
IF 5-year
1.217
CiteScore value: 2.0
CiteScore
2.0
SNIP value: 1.055
SNIP1.055
IPP value: 1.27
IPP1.27
SJR value: 0.425
SJR0.425
Scimago H <br class='widget-line-break'>index value: 28
Scimago H
index
28
h5-index value: 13
h5-index13
Supported by
Logo Leibniz Institute for Farm Animal Biology Logo Leibniz Association
Volume 46, issue 2
Arch. Anim. Breed., 46, 199–211, 2003
https://doi.org/10.5194/aab-46-199-2003
© Author(s) 2003. This work is distributed under
the Creative Commons Attribution 3.0 License.
Arch. Anim. Breed., 46, 199–211, 2003
https://doi.org/10.5194/aab-46-199-2003
© Author(s) 2003. This work is distributed under
the Creative Commons Attribution 3.0 License.

  10 Oct 2003

10 Oct 2003

13C nuclear magnetic resonance spectroscopy – a non-invasive in vivo method to measure muscle glycogen metabolism in pigs of different genotypes

A. M. Scholz1, A. D. Mitchell2, H. Song3, and P. C. Wang3 A. M. Scholz et al.
  • 1Lehr- und Versuchsgut der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München, Hubertusstr. 12, 85764 Oberschleißheim, Germany
  • 2United States Department of Agriculture, Growth Biology Laboratory Beltsville, MD 20705, USA
  • 3Howard University, Department of Radiology Washington, DC 20007, USA

Abstract. The three ryanodine receptor 1 gene variants (NN: homozygous normal, Nn: heterozygous, nn: homozygous defective) and the degree of Hampshire origin (0 %, 25 %, 50 %) serve as model for the investigation of the in vivo glycogen muscle metabolism in 27 pigs. The pigs originate from 4 different cross-breeding lines with an age varying between 41 and 58 days and a body weight between 7.3 and 19 kg. 13C nuclear magnetic resonance spectroscopy was applied non-invasively in vivo and in a few pigs also post mortem to study the metabolic processes in the biceps femoris muscle after halothane exposure. In contrast to no visible effects of the halothane challenge test, the heterozygous defective allele carriers showed a drastic reduction in the level of glycogen (57 %) coupled with an increase in body temperature (1.36 °C). Overall, these changes were intermediate compared to the dramatic response in the homozygous nn genotype and to the very slow processes in NN, considering that the drastic glycogen depletion in the heterozygous genotype occurred after a rather long time of halothane exposure ( >20 min). In addition, pigs with the highest degree of Hampshire origin (50% → RN allele frequency: ~31.5 %) showed the slowest glycogen depletion compared to pigs with a lower degree of Hampshire origin (0 or 25 %).

Download
Citation