DNA-Fingerprintuntersuchungen bei 4 Ziegenrassen zur Auswertung populationsgenetischer Parameter – einschließlich bei der gefährdeten Thüringer Wald Ziege

Abstract. Title ofthe paper: Investigations of population genetic parameters using multilocus DNA fingerprinting in 4 goat breeds in Germany
Conventional fmgerprint banding pattems were produced by Southern blotting and digoxigenm labeled oligonucleotid probes (GTG)5 and (GT)_g. Digitised images of the blots were analysed with the RFLPscan Computer program. These probes produced more bands (20–39 distinct informative bands within each group) in these goat breeds than in sheep breeds. Typically, in individual goats, an average of 8–11 bands were detected in the range of 4.3–23 kb. Analysis ofthe Polymorphie banding pattems showed that 4.5 to 8.3 genetic loci were represented by these probes. The analysis of pooled DNA samples (10–28 animals per lane) overestimated similarity values (S) between different breeds. Average similarity values within groups (based on RFLPs of individuals) ränge from 0.42–0.61, while similarity values between different groups ränge from 0.36–0.47. The results indicated that native Thüringer Wald Ziege are different from the Toggenburger goats.The Heterozygosity index (H) for each breed calculated from (GTG)5 fingerprints (H = 0.42–0.62) were in good agreement (negative correlated) with band sharing valve. These H values are lower than those obtained in a similar study of 19 sheep breeds in which the ränge was (0.68–0.82). The goat breed with the highest H value was the Bunte Deutsche Edelziege (H = 0,62). Ranking the breeds by heterozygosity index and by inversely band sharing index gives: BDE > German TOGO > WDE, TWZ > Swiss TOGO. The results are discussed with respect to breeding programs and the influence of small population size.