Sera samples from 89 dairy cows with repeat breeder and 94 healthy pregnant dairy cows (controls) from Elazig province of eastern Turkey were tested for presence of <i>Neospora caninum</i> antibodies by use of a competitive enzyme linked immunosorbent assay (cELISA). Fifteen out of 183 cows tested (8.19%) were found to be seropositive to <i>N. caninum</i> antibody. Overall, seroprevalence of <i>N. caninum</i> was significantly higher (P<0.05) in dairy cows with repeat breeder (13.48%, 12/89) than in dairy cows with healthy pregnant (3.19%, 3/94). There was no statistically significant relationship between seroprevalence and breed and age groups in cows with repeat breeder (P>0.05). In conclusion, the current study highlights the importance of <i>N. caninum</i> in cows with repeat breeder.