AAB

Archives Animal Breeding

AAB

Arch. Anim. Breed.

2363-9822

FBN Dummerstorf

Göttingen, Germany

10.5194/aab-48-32-2005

Effect of quality of sperm chromatin structure on in -vitro production of cattle embryos

Kątska-Książkiewicz

¹ Bochenek

¹ Ryńska

Department of Animal Reproduction Biotechnology, National Research Institute of Animal Production, Balice/Kraków, Poland

10 10 2005

48 1 32 39

2005

This work is licensed under the Creative Commons Attribution 3.0 Unported License. To view a copy of this licence, visit https://creativecommons.org/licenses/by/3.0/

This article is available from https://aab.copernicus.org/articles/48/32/2005/aab-48-32-2005.html

The full text article is available as a PDF file from https://aab.copernicus.org/articles/48/32/2005/aab-48-32-2005.pdf

Bull effect on results of in vitro embryo production has been well documented. The aim of the present study was to find the relationship between quality of bull sperm chromatin and its effect on in vitro embryo production. <br><br> Bovine in vitro matured oocytes were fertilized in vitro using capacitated spermatozoa (freshly ejaculated or frozen-thawed) of 12 bulls. Semen was simultaneously processed according to the sperm chromatin structure assay (SCSA) method and was analysed by flow cytometry. At least 3 replications of IVP with the same semen sample were done. The percentage of spermatozoa with abnormal chromatin ranged from 0.4% to 23.8%. All bulls used for the experiment were divided into three groups showing minimal (0.82% ± 6.82%), low (1.70% ± 15.82%) and high (18.16% ± 53.59%) percentages of spermatozoa with abnormal chromatin structure. Both cleavage rates and embryo development to the blastocyst stage were correlated significantly with sperm chromatin abnormalities and resulted in 23.1, 17.7 and 12.2% of blastocysts respectively for sperm with minimal, low and high percentages of chromatin abnormalities. The SCSA method may be used as a practical indicator of suitability of bull ejaculate for IVP purposes.